Morinda citrifolia, commonly known as noni, is a traditional natural medicine in French Polynesia and Hawaii. Functional foods derived from M. citrifolia fruit have been marketed to help prevent diseases and promote good health. The objective of this study was to assess the effects of M. citrifolia fruit on cell-mediated immunity. In the picryl chloride-induced contact dermatitis test, M. citrifolia fruit extract (Noni-ext) inhibited the suppression of cell-mediated immunity by immunosuppressive substances isolated from freeze-dried ascites of Ehrlich carcinoma-bearing mice (EC-sup). In addition, Noni-ext inhibited reduction of IL-2 production in EC-sup-treated mice and activated natural killer cells in normal mice. These results suggest that Noni-ext has multiple effects on the recovery of cell-mediated immunity. Furthermore, we investigated the active principles of Noni-ext and identified an iridoid glycoside, deacetylasperulosidic acid. Oral administration of deacetylasperulosidic acid inhibited the reduction of ear swelling, and also cancelled the suppression of IL-2 production along with the activation of natural killer cells in the same manner as that of Noni-ext.
RossIA. (2001) Medicinal plants of the world. Vol. 2. Humana Press, Totowa, 309–317.
2.
PandeM, NaikerM, MillsG, SinghN, VoroT. (2005) The Kura files: qualitative social survey. Pacific Health Dialog, 12, 85–93.
3.
NayakBS, IsitorGN, MaxwellA, BhogadiV, RamdathDD. (2007) Wound-healing activity of Morinda citrifolia fruit juice on diabetes-induced rats. Journal of Wound Care, 16, 83–86.
4.
MandukhailSU, AzizN, GilaniAH. (2010) Studies on antidyslipidemic effects of Morinda citrifolia (Noni) fruit, leaves and root extracts. Lipids in Health and Disease, 9, 88–93.
5.
MuralidharanP, KumarVR, BalamuruganG. (2010) Protective effect of Morinda citrifolia fruits on β-amyloid (25-35) induced cognitive dysfunction in mice: an experimental and biochemical study. Phytotherapy Research, 24, 252–258.
6.
OrsattiCL, MissimaF, PagliaroneAC, SforcinJM. (2010) Thl/Th2 cytokines’ expression and production by propolis-treated mice. Journal of Ethnopharmacology, 129, 314–318.
7.
CarrascoFR, SchmidtG, RomeroAL, SartorettoJL, Caparroz-AssefSM, Bersani-AmadoCA, CumanRK. (2009) Immunomodulatory activity of Zingiber officinale Roscoe, Salvia officinalis L. and Syzygium aromaticum L. essential oils: evidence for humor- and cell-mediated responses. Journal of Pharmacy and Pharmacology. 61, 961–967.
8.
NayakS, MengiS. (2010) Immunostimulant activity of noni (Morinda citrifolia) on T and B lymphocytes. Pharmaceutical Biology, 48, 724–731.
9.
KamoI, FriedmanH. (1978). Differential immunosuppressive effects of ascites fluid from mastocytoma-bearing mice on primary vs secondary immunocyte responses. The Journal of Immunology, 120, 558–562.
10.
MatsudaH, IkedaY, KuboM. (1992) Pharmacological study on Naja naja kaouthia Lesson (II). Effect of 50% ethanolic extract from dried body on phagocytic activity of mouse reticuloendothelial system. Shouyakugaku Zasshi, 46, 136–142.
11.
MajdalawiehAF, HmaidanR, CarrRI. (2010) Nigella sativa modulates splenocyte proliferation, Thl/Th2 cytokine profile, macrophage function and NK anti-tumor activity. Journal of Ethnopharmacology, 131, 268–275.
12.
XuM, ZhaoM, YangR, ZhangZ, LiY, WangJ. (2013) Effect of dietary nucleotides on immune function in Balb/C mice. International Immunopharmacology, 17, 50–56.
13.
NudoLP, CatapES. (2011) Immunostimulatory effects of Uncaria perrottetii (A. Rich.) Merr. (Rubiaceae) vinebark aqueous extract in Balb/C mice. Journal of Ethnopharmacology, 133, 613–620.
14.
IshidaN. (1985) Isolation of factors responsible for the immunosuppression found in tumor-bearing animals. Yakugaku Zasshi, 105, 91–108.
15.
MetzCH, SchroderAK, OverbeckS, KahmannL, PlümäkersB, RinkL. (2007) T-helper type 1 cytokine release is enhanced by in vitro zinc supplementation due to increased natural killer cells. Nutrition, 23, 157–163.
AkihisaT, SeinoK, KanekoE, WatanabeK, TochizawaS, FukatsuM, BannoN, MetoriK, KimuraY. (2010) Melanogenesis inhibitory activities of iridoid-, hemiterpene-, and fatty acid-glycosides from the fruits of Morinda citrifolia (Noni). Journal of Oleo Science, 59, 49–57
18.
WuMJ, ChengTL, ChengSY, LianTW, WangL, ChiouSY. (2006) Immunomodulatory properties of Grifola frondosa in submerged culture. Journal of Agricultural and Food Chemistry, 54, 2906–2914.
19.
ChenW, ZhangW, ShenW, WangK. (2010) Effects of the acid polysaccharide fraction isolated from a cultivated Cordyceps sinensis on macrophages in vitro. Cellular Immunology, 262, 69–74.
20.
WangPY, ZhuXL, LinZB. (2012) Antitumor and immunomodulatory effects of polysaccharides from broken-spore of Ganoderma lucidum. Frontiers in Pharmacology, 3, Article No. 135.
21.
HirazumiA, FurusawaE. (1999) An immunomodulatory polysaccharide-rich substance from the fruit juice of Morinda citrifolia (noni) with antitumor activity. Phytotherapy Research, 13, 380–387.
22.
MasudaM, MurataK, FukuhamaA, NarutoS, FujitaT, UwayaA, IsamiF, MatsudaH. (2009) Inhibitory effect of constituents of Morinda citrifolia seed on elastase and tyrosinase. Journal of Natural Medicines, 63, 267–273.
23.
YamazakiH, NittaK, UmezawaH. (1973) Immunosuppression induced with cell-free fluid of Ehrlich carcinoma ascites and its fractions. Gann, 64, 83–92.
24.
AshersonGL, PtakW. (1968) Contact and delayed hypersensitivity in the mouse. Immunology, 15, 405–416.
25.
AbidS, KhajuriaA, ParvaizQ, SidiqT, BhatiaA, SinghS, AhmadS, RandhawaMK, SattiNK, DuttP. (2012) Immunomodulatory studies of a bioactive fraction from the fruit of Prunus cerasus in BALB/c mice. International Immunopharmacology, 12, 626–634.
26.
ZhengL, DongGH, JinYH, HeQC. (2009) Immunotoxic changes associated with a 7-day oral exposure to perfluorooctanesulfonate (PFOS) in adult male C57BL/6 mice. Archives of Toxicology, 83, 679–689.
27.
DengS, WestBJ, PaluK, JensenCJ. (2011) Determination and comparative analysis of major iridoids in different parts and cultivation sources of Morinda citrifolia. Phytochemical Analysis, 22, 26–30.
