The chemical composition of the leaf oil of Manekia naranjoana (C. DC.) Callejas (Piperaceae) from Costa Rica was analyzed by capillary GC/FID and GC/MS. Fifty-five compounds were identified. Major compounds from the leaf oil were β-pinene (30.6%), α-pinene (18.8%), limonene (13.7%), and β-caryophyllene (6.1%). The oil presented very low toxicity to tumor and non-tumor cell lines, even though it contains components, such as α- and β-pinene, limonene and others, which have been shown to be cytotoxic. This is the first report of the chemical composition of the essential oil obtained from this species.
BurgerW. (1971) Family #41, Piperaceae. In Flora Costaricensis. Burger, W. (Ed.) Fieldiana, Botany, 35, 5-227.
2.
AriasT, Callejas-PosadaR, BornsteinA. (2006) New Combinations in Manekia, an Earlier Name for Sarcorhachis (Piperaceae). Novon, 16, 205-208.
3.
SenguptaS, RayAB. (1987)The chemistry of Piper species: a review. Fitoterapia, 58, 147-166.
4.
TripathiAK, JainDC, KumarS. (1996)Secondary metabolites and their biological and medicinal activities of Piper species plants. Journal of Medicinal and Aromatic Plant Sciences, 18, 302-321.
5.
ParmarVS, JainSC, BishtKS, JainR, TanejaP, JhaA, TyagiOD, PrasadAK, WengelJ, OlsenCE, BollPM. (1997) Phytochemistry of the genus Piper. Phytochemistry, 46, 597-673.
6.
MabberleyD. J.The Plant-Book, a Portable Dictionary of the Vascular Plants, 2nd ed. Cambridge Univ. Press, Cambridge. 1997.
7.
JaramilloMA, ManosPS. (2001)Phylogeny and patterns of floral diversity in the genus Piper (Piperaceae). American Journal of Botany, 88, 706-716.
8.
JaramilloMA, ManosPS, ZimmerEA. (2004) Phylogenetic relationships of the perianthless Piperales: reconstructing the evolution of floral development. International Journal of Plant Sciences, 165, 403-416.
9.
TreleaseW. (1927) The Piperaceae of Panama. Contributions from the U.S. National Herbarium, 26, 15-50.
10.
TreleaseW. (1929) The Piperaceae of Costa Rica. Contributions from the U.S. National Herbarium, 26, 115-226.
11.
WankeS, JaramilloMA, BorschT, SamainMS, QuandtD, NeinhuisC. (2007) Evolution of Piperales -matK gene and trnK intron sequence data reveal lineage specific resolution contrast. Molecular Phylogenetics and Evolution, 42, 477-497.
12.
WilliamsC, EspinosaOA, MontenegroH, CubillaL, CapsonTL, Ortega-BarríaE, RomeroLI. (2003) Hydrosoluble Formazan XTT: its application to natural products drug discovery for Leishmania. Journal of Microbiological Methods, 55, 813-816.
13.
FernandesG. G.Avaliação fitoquímica de Sarcorhachis obtusa (Piperaceae). MSc. Thesis. Departamento de Química, Universidade Federal do Paraná, 2006. http://hdl.handle.net/1884/7260
14.
EsiyokD, ÖtlesS, AkcicekE. (2004) Herbs as a food source in Turkey. Asian Pacific Journal of Cancer Prevention, 5, 77-82.
15.
EdrisAE. (2007) Pharmaceutical and therapeutic potentials of essential oils and their individual volatile constituents: a review. Phytotherapy Research, 21, 308-323.
16.
KorochAR, JulianiHR, ZygadloJA. (2007) Bioactivity of essential oils and their components. In Flavours and Fragrances. Chemistry, bioprocessing and sustainability; BergerRG. (Ed.), Springer: Berlin, p. 87-115.
17.
PoloMP, de BravoMG. (2006) Effect of geraniol on fatty-acid and mevalonate metabolism in the human hepatoma cell line Hep G2. Biochemistry and Cell Biology, 84, 102-111.
18.
ZainalAriffin SH, Wan OmarWhh, ZainalAriffin Z, SafianMF, SenafiS, Megat AbdulWahab R. (2009) Intrinsic anticarcinogenic effects of Piper sarmentosum ethanolic extract on a human hepatoma cell line. Cancer Cell International, 9, no pp. given. http://www.cancerci.com/content/9/1/6.
19.
da SilvaMota J, LeiteAC, BatistaJunior JM, NoelíLópez S, LuzAmbrósio D, DuóPasserini G, KatoMJ, da SilvaBolzani V, BarrettoCicarelli RM, FurlanM. (2009) In vitro trypanocidal activity of phenolic derivatives from Peperomia obtusifolia. Planta Medica, 75, 620-623.
20.
WerkaJS, BoehmeAK, SetzerWN. (2007) Biological activities of essential oils from Monteverde, Costa Rica. Natural Product Communications, 2, 1215-1219.
21.
SetzerWN, ParkG, AgiusBR, StokesSL, WalkerTM, HaberWA. (2008)Chemical compositions and biological activities of leaf Essentials oils of twelve species of Piper from Monteverde, Costa Rica. Natural Product Communications, 3, 1367-1374.
van den DoolH, KratzPD. (1963) A generalization of the retention index system including linear temperature programmed gas-liquid partition chromatography. Journal of Chromatography A, 11, 463-471.
24.
McLaffertyFW. (1993) Registry of Mass Spectral Data, John Wiley & Sons: New York.
25.
AdamsRP. (2007) Identification of Essential Oil Components by Gas Chromatography/ Quadrupole Mass Spectroscopy, 4th ed., Allured: Carol Stream IL.
26.
DenizotF, LangR. (1986) Rapid colorimetric assay for cell growth and survival, modifications to the tetrazolium dye procedure giving improved sensitivity and reliability. Journal of Inmunological Methods, 89, 271-277.
