One new flavonoid glycoside, 3-O-kaempferol 4-O-(galloyl)-β-D-glucoside, one new bergenin derivative, 11-0-caffeoylbergenin, along with other known flavonoids and phenolic derivatives, were isolated from the leaves of Securinega virosa. Their structures were established on the basis of detailed spectral analysis. In vitro biological analysis of the isolated compounds showed that they were able to quench DPPH radicals and had a direct scavenging activity on superoxide anion. Kaempferol 3-O-(4-galloyl)-β-D-glucopyranoside (1), 11-0-caffeoylbergenin (2), and glucogallin (6) exhibited the highest antioxidant capacity, being also able to modulate hydroxyl radical formation more efficiently than the other compounds, acting as direct hydroxyl radical scavengers and chelating iron.
AdjanohounEJ, AkéAssi L, FloretJJ, GuindoS, KoumaréM, AhyiAMR, RaynalJ. (1985) Contribution aux études ethnobotaniques et floristiques au Mali. Médecine traditionnelle et pharmacopée. ACCT Ed, Paris, 250 p.
2.
KerharoJ, AdamJC. (1974) Pharmacopée sénégalaise traditionnelle: Plantes médicinales et toxicologiques.Paris Vigot et Frères Ed, 1011 p.
3.
BhakuniDS, DharML, DharMM, DhawanBN, MehrotraBN. (1969) Screening of Indian plants for biological activity. Part II. Indian Journal of Experimental Biology, 7, 250–262.
4.
TatematsuH, MoriM, YangTH, ChangJJ, LeeTTY, LeeKH. (1991) Cytotoxic principles of Securinega virosa: virosecurinine and viroallosecurinine and related derivatives. Journal of Pharmaceutical Science, 80, 325–327.
5.
ParisRR, MoyseH, Le MenJ. (1955) Alkaloids from new Euphorbiaceae: Flueggea virosa. Annales Pharmaceutiques Francaises13, 245–250.
6.
FicoG, BracaA, De TommasiN, TomèF, MorelliI. (2001) Flavonoids from Aconituym napellus subsp. neomontanum. Phytochemistry, 57, 543–546.
7.
NawwarMAM, HusseinSAM, MerfortI. (1994) NMR spectral analysis of polyphenolics from Punica granatum. Phytochemistry36, 793–798.
8.
LiuY, CuiC, ZhaoM, WangJ, LuoW, YangB, JiangY. (2008) Identification of phenolics in the fruit of emblica (Phyllanthus emblica L.) and their antioxidant activities. Food Chemistry, 109, 909–915.
9.
KashiwadaY, NonakaG, NishiokaI. (1984) Isolation and characterization of new dimeric and trimeric procyanidins. Chemical & Pharmaceutical Bulletin, 32, 3461–3470.
10.
AdesinaSK, IdowuO, OgundainiAO, OladimejiH, OlugbadeTA, OnawunmiGO, PiasM. (2000) Antimicrobial constituents of the leavs of Acalypha wilkesiana and Acalypha hispida. Phytotherapy Research, 14, 371–374.
11.
LeeYY, JangDS, JinJL, Yun-ChoiHS. (2005) Anti-platelet aggregating and antioxidative activities of 11-O-(4′-O-methylgalloyl)-bergenin a new compound isolated from Crassula cv. ‘Himaturi’. Planta Medica, 71, 776–777.
12.
BorsW, MichelC. (1999) Antioxidant capacity of flavanols and gallate esters: pulse radiolysis studies. Free Radical Biology & Medicine, 27, 1413–1426.
13.
KinoshitaS, InoueY, NakamaS, IchibaT, AniyaY. (2007) Antioxidant and hepatoprotective actions of medicinal herb, Terminalia catappa L. from Okinawa Island and its tannin corilagin. Phytomedicine, 14, 755–762.
14.
MaldonadoPD, Chánez-CárdenasME, Pedraza-ChaverríJ. (2005) Aged garlic extract, garlic powder extract, S-allylcysteine, diallyl sulfide and diallyl disulfide do not interfere with the antibiotic activity of gentamicin. Phytotherapy Research, 19, 252–254.
15.
HalliwellB, GutteridgeJM. (1995) The definition and measurement of antioxidants in biological systems. Free Radical Biology & Medicine, 18, 125–126.
16.
LattéKP, KolodziejH. (2004) Antioxidant properties of phenolic compounds from Pelargonium reniforme. Journal of Agricultural and Food Chemistry52, 4899–4902.
17.
PaolettiF, AldinucciD, MocalliA, CaparriniA. (1986) A sensitive spectrophotometric method for the determination of superoxide dismutase activity in tissue extracts. Analytical Biochemistry, 154, 536–541.
18.
RussoA, AcquavivaR, CampisiA, SorrentiV, Di GiacomoC, VirgataG. (2000) Bioflavonoids as antiradicals, antioxidants and DNA cleavage protectors. Cell Biology and Toxicology, 16, 91–98.
19.
McGownEL, RusnakMG, LewisCM, TillotsonJA. (1982) Tissue ascorbic acid analysis using ferrozine compared with the dinitrophenylhydrazine method. Analytical Biochemistry, 119, 55–61.
