This work deals with ultrasonically assisted extraction (UAE) of biologically active compounds from rhizomes of Rhodiola rosea, a popular medicinal plant. The influence of temperature, type of solvent and solid/solvent ratio on the yield of total extracts, total phenols and flavonoids was established. The best extraction of total phenols and flavonoids was achieved by using 50% aqueous EtOH and MeOH, respectively. Five times increase of solid/solvent ratio (from 1:20 to 1:100 (w/v)) leads to slow increase of the yield of total phenols and flavonoids. The extraction effectiveness of conventional maceration with 50% EtOH and UAE performed for 1 h at 25°C using the same solvent with respect of total phenols was comparable.
PetkovVD, YonkovD, MosharoffA, KambourovaT, AlovaL, PetkovVV, TodorovI. (1986) Effects of alcohol aqueous extract from Rhodiola rosea L. roots on learning and memory. Acta physiologica et Pharmacologica Bulgarica, 12, 3–16.
2.
KhanumF, BawaAS, SinghB. (2005) Rhodiola rosea: A versatile adaptogen. Comprehensive Reviews in Food Science and Food Safety, 4, 55–62.
3.
VinatoruM, TomaM, RaduO, FilipPI, LazurcaD, MasonTJ. (1997) The use of ultrasound for the extraction of bioactive principles from plant materials. Ultrasonics Sonochemistry, 4, 135–139.
4.
VinatoruM, TomaM, MasonTJ. (1999) Ultrasonically assisted extraction of bioactive principles from plants and their constituents. Advances in Sonochemistry, 5, 209–247.
5.
ProestosC, KomaitisM. (2006) Ultrasonically assisted extraction of phenolic compounds from aromatic plants: comparison with conventional extraction techniques. Journal of Food Quality, 29, 567–582.
6.
AsamiDK, HongY-J, BarrettDM, MitchellAE. (2003) Comparison of the total phenolic and ascorbic acid content of freeze-dried and air-dried marionberry, strawberry, and corn grown using conventional, organic and sustainable agricultural practices. Journal of Agricultural and Food Chemistry, 51, 1237–1241.
7.
PriorRL, WUX, SchaichK. (2005) Standardized methods for the determination of antioxidant capacity and phenolics in foods and dietary supplements. Journal of Agricultural and Food Chemistry, 53, 4290–4302.
8.
MiliauskasG, VenkutonisPR, van BeekTA. (2004) Screening of radical scavening activity of some medicinal and aromatic plant extracts. Food Chemistry, 85, 231–237.
