The influence of the extraction method on the yield and composition of extracts of Sideritis (Pirin mountain tea) has been studied. Maceration, ultrasound-assisted (USAE) and microwave assisted extraction (MAE) were applied. Total phenolics and total flavonoids were quantified spectrophotometrically, and individual compounds were analyzed by HPLC-DAD-MSn. This preliminary study reveals that the traditional way of tea preparation from Sideritis is the most appropriate in order to extract the maximum of total flavonoids and total phenolics. In the case of methanol extraction, the optimal method is USAE
KirimerN, BaserKHC, DemirciB, DumanH. (2004) Essential oils of Sideritis species of Turkey belonging to the section Empedoclia. Chemistry of Natural Compounds, 40, 19–23.
2.
VillarA, EspluguesJ, AlcarazMJ. (1982) Acute antiinflammatory activity of Sideritis mugronensis flavonoid Archivos de Farmacologia y Toxicologia, 8, 99–106.
3.
KolevaII, LinssenJPH, van BeekTA, EvstatievaLN, KortenskaV, HandjievaN. (2003) Antioxidant activity screening of extracts from Sideritis species (Labiatae) grown in Bulgaria. Journal of the Science of Food and Agriculture, 83, 809–819.
4.
GabrieliCN, KefalasPG, KokkalouEL. (2005) Antioxidant activity of flavonoids from Sideritis raeseri. Journal of Ethnopharmacology, 96, 423–428.
5.
GuvencA, HoughtonPJ, DumanH, CoskunM, SahinP. (2005) Antioxidant activity studies on selected Sideritis species native to Turkey. Pharmaceutical Biology, 43, 173–177.
6.
OzkanG, SagdicO, OzcanM, OzcelikH, UnverA. (2005) Antioxidant and antibacterial activities of Turkish endemic Sideritis extracts. Grasas y Aceites, 56, 16–20.
7.
HuieCW. (2002) A review of modern sample-preparation techniques for the extraction and analysis of medicinal plants. Analytical and Bioanalytical Chemistry, 37, 23–30.
8.
KaufmannB, ChristenP. (2002) Recent extraction techniques for natural products: Microwave-assisted extraction and pressurized solvent extraction. Phytochemical Analysis, 13, 105–113.
9.
EvstatievaL, KolevaI. (2000) Cultivation of S. scardica Griseb. Proceedings of the First Conference on Medicinal and Aromatic plants of Southeast Europe, Arandelovac, Yugoslavia, May 29 - June 03, pp. 189–195.
