The cytotoxicity of three cycloartane triterpenoids, 9, 19-cycloart-7β, 24R, 25- triol-1-en-3-one (1), 9, 19-cycloart-7β, 24R, 25-triol-1-en-3-one 25-O-β-D-glucopynanoside (2), and 25-O-β-D-arabinopyranosyl-(1→4)- β-D-glucopyranosyl-9, 19-cycloart-7β, 24R, 25-triol-1-en-3-one (3), isolated from Sphaerophysa salsula was investigated on SF188, U87wt, MCF-7, and H460 cancer cell lines. Compound 1 showed the strongest activity. Cell cycle analysis was employed to elucidate the cytotoxicity on the tested U87wt cells, which led to G2/M arrest. In addition, from the Western blotting experiments, the expression of P21 is increased.
MaZJ, LiX, LuY, WangC. (2003) A new cycloartane from Sphaerophysa salsula. Chinese Chemical Letters, 14, 594–596.
4.
MaZJ, FujiiI, EbizukaY, LiX. (2003) A new lignan from the seeds ofSphaerophysa salsula. Natural Products Research, 17, 423–425.
5.
MaZJ, MengDL, WangYX, LiX. (2003) Chemical constituents from Sphaerophysa salsula. Journal of Shenyang Pharmaceutical University, 20, 104–106.
6.
MaZJ, FujiiI, EbizukaY, LiX. (2004) Flavonoids from the seeds ofSphaerophysa salsula. Journal of Asian Natural Products Research, 6, 69–73.
7.
MaZJ, FujiiI, EbizukaY, LiX. (2004) Two new 9, 10-seco-cycloartanes from the seeds of Sphaerophysa salsula.Chinese Chemical Letters, 15, 58–60.
8.
MaZJ, FujiiI, EbizukaY, LiX. (2004) A new lignan from the seeds ofSphaerophysa salsula. Journal of Asian Natural Products Research, 6, 265–269.
9.
MaZJ, LiX, WangJH. (2004) Cycloartane-type triterpenoids from Sphaerophysa salsula. Journal of Asian Natural Products Research, 6, 259–264.
10.
MaZJ, FujiiI, EbizukaY, LiX. (2006) Two new 9, 10-seco-cycloartanes from the seeds ofSphaerophysa salsula., Journal of Asian Natural Products Research, 8, 657–661.
11.
TianZ, YangMS, HuangF, LiKG, SiJY, ShiL, ChenSB, XiaoPG. (2005) Cytotoxicity of three cycloartane triterpenoids from Cimicifuga dahurica, Cancer Letters, 226, 65–75.
12.
LaemmliUK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4, Nature, 227, 680–685.