From the methanolic extract of Crocus sativus petals nine known flavonoids have been isolated and identified, including glycosidic derivatives of quercetin and kaempferol as major compounds (1–2), and their methoxylated and acetylated derivatives. Additionally, LC-ESI-MS qualitative and LC-ESI-MS/MS quantitative studies of the major compounds of the methanolic extract were performed. The high content of glycosylated flavonoids could give value to C. sativus petals, which are a waste product in the production of the spice saffron.
FernandezJA. (2004) Biology, biotechnology and biomedicine of saffron. Recent Research Developments in Plant Science, 2127–159
2.
RiosJL, RecioMC, GinerRM, ManetsS. (1996) An update review of saffron and its active constituents. Phytotheapy Research, 10, 189–193
3.
XiL, QianZ. (2006) Pharmacological properties of crocetin and crocin (digentiobiosyl esters of crocetin) from saffron, Natural Product Communications, 1, 65–75.
4.
LiCY, LeeEJ, WuTS. (2004) Antityrosinase principles and constituents of the petals of Crocus sativus. Journal of Natural Products, 67, 437–440
5.
Rice-EvansCA, MillerNJ, PagangaG. (1997) Antioxidant properties of phenolic compounds. Trends in Plant Science, 2, 152–159.
6.
CaoG, SoficE, PriorRL. (1997) Antioxidant and pro-oxidant behavior of flavonoids: structure-activity relationships. Free Radical Biology and Medicine, 22, 749–760
7.
Rice-EvansCA, MillerNJ, PanagaG. (1996) Structure-antioxidant activity relationships of flavonoids and phenolic acids. Free Radical Biology and Medicine, 20, 933–956
8.
LienEJ, RenS, BuiH, WangR. (1999) Quantitative structure-activity relationship analysis of phenolic antioxidants. Free Radical Biology and Medicine, 26, 285–294
9.
MontoroP, BracaA, PizzaC, De TommasiN. (2005) Structure-antioxidant activity relationships of flavonoids isolated from different plant species. Food Chemistry, 92, 349–355.
10.
ICH Q2B, International Conference on Harmonisation, London, 1995.
11.
LiX, XiongZ, YingX, CuiL, ZhuW, LiF. (2006) A rapid ultra-performance liquid chromatography-electrospray ionization tandem mass spectrometric method for the qualitative and quantitative analysis of the constituents of the flower of Trollius ledibouri Reichb. Analytica Chimica Acta, 580, 170–180
12.
BenavidesA, MontoroP, BassarelloC, PiacenteS, PizzaC. (2006) Catechin derivatives in Jatropha macrantha stems: Characterisation and LC/ESI/MS/MS quali-quantitative analysis. Journal of Pharmaceutical and Biomedical Analysis, 40, 639–647
13.
MontoroP, TuberosoCIG, PerroneA, PiacenteS, CabrasP, PizzaC. (2006) Characterisation by liquid chromatography -electrospray tandem mass spectrometry of anthocyanins in extracts of Myrtus communis L. berries used for the preparation of myrtle liqueur. Journal of Chromatography A, 1112, 232–240.
14.
MerfortI, WendischD. (1992) New flavonoid glycosides from Arnicae flos DAB 9. Planta Medica, 58, 355–357.
15.
GrouillerA, PachecoH. (1967) Flavonoid compounds. VI. Nuclear magnetic resonance spectra of some O-glucosylflavonals, their aglycons and three synthetic mono- and di- O –glucosylflavanones. Bulletin de la Societe Chimique de France, 6, 1938–1943.
16.
NawwarMAM, El-MousallamyAMD, BarakatHH. (1989) Quercetin 3-glycosides from the leaves of Solanum nigrum. Phytochemistry, 28, 1755–1757.
17.
SenatoreF, D'AgostinoM, DiniI. (2000) Flavonoid glycosides of Barbarea vulgaris L. (Brassicaceae). Journal of Agricultural and Food Chemistry, 48, 2659–2662.