Three new oleanane saponins (1–3), together with four known ones (4–7), were isolated from the aerial parts of Polyscias guilfoylei. Their structures were elucidated by 1D and 2D NMR experiments, including 1D TOCSY, DQF-COSY, ROESY, HSQC, and HMBC spectroscopy, as well as ESIMS analysis. The antiproliferative activity of all compounds was evaluated using three murine and human cancer cell lines; J774.A1, HEK-293, and WEHI-164. All the compounds were inactive except for 3β-O-[β-D-glucopyranosyl-(1→2)-α-L-arabinopyranosyl]-echinocystic acid 28-[O-β-D-glucopyranosyl-(1→6)O-β-D-glucopyranosyl] ester (3), which was active against all the cell lines.
De TommasiN, PizzaC, BellinoA, VenturellaP. (1997) Triterpenoid saponins from Schefflera divaricata. Jounal of Natural Products, 60, 663–668.
2.
De TommasiN, PizzaC, BellinoA, VenturellaP. (1997) Triterpenoid saponins from Trevesia sundaica. Journal of Natural Products, 60, 1070–1074.
3.
CioffiG, BracaA, AutoreG, MorelliI, PintoA, VenturellaF, De TommasiN. (2003) Cytotoxic saponins from Schefflera fagueti. Planta Medica, 69, 750–756.
4.
PlunkettGM, SoltisDE, SoltisPS. (1996) Higher level relationships of Apiales (Apiaceae and Araliaceae) based on phylogenetic analysis of rbcL sequences. American Journal of Botany, 83, 499–515.
5.
OpipariAWJ, HuHM, YabkowitzR, DixitYM. (1992) The A20 zinc finger protein protects cells from tumor necrosis factor cytotoxicity. Journal of Biological Chemistry, 267, 12424–12427.
6.
Dal PiazF, De LeoM, BracaA, De SimoneF, MorelliI, De TommasiN. (2005) Electrospray ionization mass spectrometry for identification and structural characterization of pregnane glycosides. Rapid Communications in Mass Spectrometry, 19, 1041–1052.
7.
De TommasiN, PiacenteS, De SimoneF, PizzaC. (1993) Characterization of three new triterpenoid saponins from Ardisia japonica. Journal of Natural Products, 56, 1669–1675.
8.
YoshikawaK, SuzakiY, TanakaM, AriharaS, NigamSK. (1997) Three acylated saponins and a related compound from Pithecellobium dulce. Journal of Natural Products, 60, 1269–1274.
JiangW, LiW, HanL, LiuL, ZhangQ, ZhangS, NikaidoT, KoikeK. (2006) Biologically active triterpenoid saponins from Acanthopanax senticosus. Jounal of Natural Products, 69, 1577–1581.
12.
MiyaseT, SutohN, ZhangDM, UenoA. (1996) Araliasaponins XII–XVIII, triterpene saponins from the roots of Aralia chinensis Phytochemistry, 42, 1123–1130.
13.
GreenLM, ReadeJL, WareCF. (1984) Rapid colorimetric assay for cell viability: application to the quantitation of cytotoxic and growth inhibitory lymphokines. Journal of Immunological Methods, 70, 257–268.
