An HPLC/DAD/ESI/MS method was established for the qualitative analysis of the major constituents in Shi-Quan-Da-Bu decoction, a traditional Chinese medicinal formula. Based on the chromatographic separation of most of the constituents of Shi-Quan-Da-Bu decoction on a Zorbax SB-C18 column with water-acetonitrile as the mobile phase, more than 150 components were detected by LC-MS. Among them, 36 major components, including flavonoids, triterpenoid saponins, iridoid glycosides, phathalides and phenylpropionic acids were either identified or tentatively characterized. Twenty one compounds were identified by ESI-MS and comparison of their retention times with those of reference standards. The other 15 compounds were deduced according to their MS and UV spectra and retention behaviors by comparing the results with those reported in the literature. Most of these compounds were derived mainly from Paeonia lactiflora, Glycyrrhiza uralensis, Panax ginseng, Ligusticum chuanxiong, Angelica sinensis and Astragalus membranaceus.
DrasarP, MoravcovaJ. (2004) Recent advances in analysis of Chinese medical plants and traditional medicines. Journal of Chromatography B, 812, 3–21.
2.
ZhangJL, CuiM, HeY, YuHL, GuoDA. (2005) Chemical fingerprint and metabolic fingerprint analysis of Danshen injection by HPLC-UV and HPLC-MS methods. Journal of Pharmaceutical and Biomedical Analysis, 36, 1029–1035.
3.
YeM, GuoDA, YeG, HuangCG. (2005) Analysis of homoisoflavonoids in Ophiopogon japonicus by HPLC-DAD-ESI-MSn. Journal of the American Society for Mass Spectrometry, 16, 234–243.
4.
YeM, GuoDA. (2005) Investigations of taxoids by electrospray and atmospheric pressure chemical ionization with tandem mass spectrometry. Rapid Communications in Mass Spectrometry, 19, 818–824.
5.
YeM, GuoDA. (2005) Analysis of bufadienolides in the Chinese drug ChanSu by high-performance liquid chromatography with atmospheric pressure chemical ionization tandem mass spectrometry. Rapid Communications in Mass Spectrometry, 19, 1881–1892.
6.
YeM, YanYN, GuoDA. (2005) Characterization of phenolic compounds in the Chinese herbal drug Tu-Si-Zi by liquid chromatography coupled to electrospray ionization mass spectrometry. Rapid Communications in Mass Spectrometry, 19, 1469–1484.
7.
LiuRX, YeM, GuoHZ, BiKS, GuoDA. (2005) Liquid chromatography/electrospray ionization mass spectrometry for the characterization of twenty-three flavonoids in the extract of Dalbergia odorifera. Rapid Communications in Mass Spectrometry, 19, 1557–1565.
8.
HanJ, YeM, ChenHB, DaiSJ, GuoDA. (2005) Determination of diterpenoids and flavonoids in Isodon rubescens by LC-ESI-MS-MS. Chromatographia, 62, 203–207.
9.
YangM, LiuAH, GuanSH, SunJH, XuM, GuoDA. (2006) Characterization of tanshinones in the roots of Salvia miltiorrhiza (Dan-shen) by high-performance liquid chromatography with electrospray ionization tandem mass spectrometry. Rapid Communications in Mass Spectrometry, 20, 1266–1280.
10.
YeM, HanJ, ChenHB, ZhengJH, GuoDA. (2007) Analysis of phenolic compounds in rhubarbs using liquid chromatography coupled with electrospray ionization mass spectrometry. Journal of the American Society for Mass Spectrometry, 18, 82–91.
11.
LiuAH, LinYH, YangM, GuoH, GuanSH, SunJH, GuoDA. (2007) Development of the fingerprints for the quality of the roots of Salvia miltiorrhiza and its related preparations by HPLC-DAD and LC-MSn. Journal of Chromatography B, 846, 32–41.
12.
HanJ, YeM, XuM, SunJH, WangBR, GuoDA. (2007) Characterization of flavonoids in the traditional Chinese herbal medicine-Huangqin by liquid chromatography coupled with electrospray ionization mass spectrometry. Journal of Chromatography B, 848, 355–362.
13.
GuoH, LiuAH, YeM, YangM, GuoDA. (2007) Characterization of phenolic compounds in the fruits of Forsythia suspensa by high-peformance liquid chromatography coupled with electrospray ionization tandem mass spectrometry. Rapid Communications in Mass Spectrometry, 21, 715–729.
14.
YangM, WangXM, GuanSH, XiaJM, SunJH, GuoH, GuoDA. (2007) Analysis of triterpenoids in Ganoderma lucidum using liquid chromatography coupled with electrospray ionization mass spectrometry. Journal of the American Society for Mass Spectrometry, 18, 927–939.
15.
HanJ, YeM, GuoH, YangM, WangBR, GuoDA. (2007) Analysis of multiple constituents in a Chinese herbal preparation Shuang-Huang-Lian oral liquid by HPLC-DAD-ESI-MSn. Journal of Pharmaceutical and Biomedical Analysis, 44, 430–438.
16.
LiuAH, GuoH, YeM, LinYH, SunJH, XuM, GuoDA. (2007) Detection, characterization and identification of phenolic acids in Danshen using high-performance liquid chromatography with diode array detection and electrospray ionization mass spectrometry. Journal of Chromatography A, 1161, 170–182.
17.
Zee-ChengRK. (1992) Shi-quan-da-bu-tang (ten significant tonic decoction), SQT. A potent Chinese biological response modifier in cancer immunotherapy, potentiation and detoxification of anticancer drugs. Methods and Findings in Experimental and Clinical Pharmacology, 14, 725–736.
18.
NiwaK, HashimotoM, MorishitaS, LianZL, TagamiK, MoriH, TamayaT. (2001) Preventive effects of Juzen-taiho-to on N-methyl-N-nitrosourea and estradiol-17β-induced endometrial carcinogenesis in mice. Carcinogenesis, 22, 587–591.
19.
DaiY, KatoM, TakedaK, KawamotoY, AkhandAA, HossainK, SuzukiH, NakashimaI. (2001) T-cell-immunity-based inhibitory effects of orally administered herbal medicine Juzen-taiho-to on the growth of primarily developed melanocytic tumors in RET-transgenic mice. Journal of Investigative Dermatology, 117, 694–701.
20.
TagamiK, NiwaK, LianZL, GaoJC, MoriH, TamayaT. (2004) Preventive effect of Juzen-taiho-to on endometrial carcinogenesis in mice is based on Shimotsu-to constituent. Biological and Pharmaceutical Bulletin, 27, 156–161.
21.
KamiyamaH, TakanoS, IshikawaE, TsuboiK, MatsumuraA. (2005) Anti-angiogenic and immunomodulatory effect of the herbal medicine “Juzen-taiho-to” on malignant glioma. Biological and Pharmaceutical Bulletin, 28, 2111–2116.
22.
GaoXR, TianGY. (2006) Active principles of Paeonia lactiflora Pall.. Chinese Journal of New Drugs, 15, 416–418.
23.
LiSL, LinG, ChungHS, TamYK. (2004) Study on fingerprint of rhizome Chuanxiong by HPLC-DAD-MS. Acta Pharmacologica Sinica, 39, 621–626.
24.
LaoSC, LiSP, KanKKW, LiP, WanJB, WangYT, DongTTX, TsimKWK. (2004) Identification and quantification of 13 components in Angelica sinensis (Danggui) by gas chromatography-mass spectrometry coupled with pressurized liquid extraction. Analytica Chimica Acta, 526, 131–137.
25.
LiJ, ZhangLP, ZhangZL. (2005) Recent development of Radix rehmanniae. Journal of He Nan University of Chinese Medicine, 20, 79–83.
26.
FuzzatiN, GabettaB, JayakarK, PaceR, PeterlongoF. (1999) Liquid chromatography-electrospray mass spectrometric identification of ginsenosides in Panax ginseng roots. Journal of Chromatography A, 854, 69–79.
27.
ZhouY, WangMK, LiaoX, ZhuXM, PengSL, DingLS. (2004) Rapid identification of compounds in Glycyrrhiza Uralensis by liquid chromatography/tandem mass spectrometry. Chinese Journal of Analytical Chemistry, 32, 174–178.
28.
GuoFQ, HuangLF, ZhouSY, ZhangTM, LiangYZ. (2006) Comparison of the volatile compounds of Atractylodes medicinal plants by headspace solid-phase microextraction-gas chromatography-mass spectrometry. Analytical Chimica Acta, 570, 73–78.
29.
ZhangSF, LiuJH, JiangJQ, ZhouYM. (2005) A review of chemical constituents, medicinal function, development and utilization of Poria cocos. Chinese Journal of the Practical Chinese with Modern Medicine, 18, 227–230.
30.
HuangJW. (2005) Study on chemical components of essential oils in Cinnamomum cassia Presl from different growth years by GC-MS. Chinese Journal of Pharmaceutical Analysis, 25, 288–291.
31.
LiR, FuTJ, JiYQ, DingLS, PengSL. (2005) A study of roots of Astragalus mongholicus and Astragalus membranaceus by high performance liquid chromatography-mass spectrometry. Chinese Journal of Analytical Chemistry, 33, 1676–1680.
32.
The State Commission of Chinese Pharmacopoeia. (2005) Pharmacopoeia of People's Republic of China, Chemical Industry Press, Beijing, 1, 298–299.
33.
GaoJL, LeungKSY, WangYT, LaiCM, LiSP, HuLF, LuGH, JiangZH, YuZL. (2007) Qualitative and quantitative analyses of nucleosides and nucleobases in Ganoderma spp. by HPLC-DAD-MS. Journal of Pharmaceutical and Biomedical Analysis, 44, 807–811.
34.
ZhangHJ, ChengYY. (2006) An HPLC/MS method for identifying major constituents in the hypocholesterolemic extracts of Chinese medicine formula ‘Xue-Fu-Zhu-Yu decoction’. Biomedical Chromatography, 20, 821–826.
35.
KanedaM, litakaY, ShibataS. (1972) The absolute structures of paeoniflorin, albiflorin, oxypaeoniflorin and benzoyl-paeoniflofin isolated from Chinese paeony root” should be replaced by “Chemical studies on the oriental plant drugs—XXXIII The absolute structures of paeoniflorin, albiflorin, oxypaeoniflorin and benzoylpaeoniflorin isolated from Chinese paeony root. Tetrahedron, 28, 4309–4317.
36.
ZhangHJ, ShenP, ChengYY (2004) Identification and determination of the major constituents in traditional Chinese medicine Si-Wu-Tang by HPLC coupled with DAD and ESI-MS. Journal of Pharmaceutical and Biomedical Analysis, 34, 705–713.
37.
XiaoHB, KruckerM, AlbertK, LiangXM. (2004) Determination and identification of isoflavonoids in Radix astragali by matrix solid-phase dispersion extraction and high-performance liquid chromatography with photodiode array and mass spectrometric detection. Journal of Chromatography A, 1032, 117–124.
38.
LinLZ, HeXG, LindenmaierM, NolanG, YangJ, ClearyM, QiuSX, CordellGA. (2000) Liquid chromatography-electrospray ionization mass spectrometry study of the flavonoids of the roots of Astragalus mongholicus and A. membranaceus. Journal of Chromatography A, 876, 87–95.
39.
LiuQ, LiuYL. (1989) Studies on chemical constituents of Glycyrrhiza eurycarpa P. C. Li. Acta Pharmacologica Sinica, 24, 525–531.
40.
LiuQ, LiuYL. (1991) Studies on chemical constituents of Glycyrrhiza eurycarpa P. C. Li (II). Acta Botanica Sinica, 33, 314–322.
41.
YiT, LeungKSY, LuGH, ChanK, ZhangH. (2006) Simultaneous qualitative and quantitative analyses of the major constituents in the rhizome of Ligusticum chuanxiong using HPLC-DAD-MS. Chemical and Pharmaceutical Bulletin, 54, 255–259.
42.
ReedGA, DimmelDR, MalcolmEW. (1993) Influence of nucleophiles on the high temperature aqueous isomerization of cis- to trans-cinnamic acid. Journal of Organic Chemistry, 58, 6364–6371.
43.
LiuY, YangJS, CaiZW. (2006) Chemical investigation on Sijunzi decoction and its two major herbs Panax ginseng and Glycyrrhiza uralensis by LC/MS/MS. Journal of Pharmaceutical and Biomedical Analysis, 41, 1642–1647.
44.
ZschockeS, KlaiberI, BauerR, VoglerB. (2005) HPLC-coupled spectroscopic techniques (UV, MS, NMR) for the structure elucidation of phthalides in Ligusticum chuanxiong. Molecular Diversity, 9, 33–39.
45.
YangL, HeKJ, YangY, LuanHW (2001) A preparation method of ginsenoside Rg3 with acid hydrolysis. China Patent, 01133409.6.
46.
YangL, HeKJ, YangY. (2003) A preparation method of less polar individual ginsenosides and saponins by base hydrolysis. China Patent CN03134090.3.
47.
KimWY, KimJM, HanSB, LeeSK, KimND, ParkMK, KimCK, ParkJH. (2000) Streaming of ginseng at high temperature enhances biological activity. Journal of Natural Products, 63, 1702–1704.
48.
HeLX, HuangZJ, TanN, LinYC. (2006) Changes of chemistry constituents of Fumai decoction made by decoction together and single on HPLC. Acta Scientiarum Naturalium Universitatis Sunyatseni, 45, 120–122.
49.
XiaY, LiZM, ZhuDN, YanYQ. (1998) Relationship between components changes and efficacy of Shengmaisan. Chemical researches on Shengmaisan I. China Journal of Chinese Material Medica, 23, 230–231.
50.
ZhuDN, LiZM, YanYQ, ZhuJG. (1998) Relationship between components changes and efficacy of Shengmaisan. Chemical researches on Shengmaisan II. China Journal of Chinese Material Medica, 23, 291–293.
51.
ZhuDN, YanYQ, LiZM. (1998) Relationship between components changes and efficacy of Shengmaisan. Chemical researches on Shengmaisan III. China Journal of Chinese Material Medica, 23, 483–485.
52.
YueL, ZhuDN, YanYQ, YuBY. (2006) Relationship between components changes and efficacy of Shengmaisan VII. Chemical dynamic change of schisandrin in Shengmaisan. China Journal of Chinese Material Medica, 31, 1010–1012.
53.
LuYR, YangZK, DongYM. (1996) Studies on changes of chemical constituents of Radix Sophorae Flavescentis in complex formulas of traditional Chinese medicine. China Journal of Chinese Material Medica, 21, 412–414.
