Free accessResearch articleFirst published online 2007-8
In Vitro Plant Growth Promoting Activity of Phyllocladane Diterpenoids Isolated from Callicarpa macrophylla Vahl. in Shoot Cultures of Rauwolfia serpentina
Phyllocladane diterpenoids, particularly calliterpenone (1) and calliterpenone monoacetate (2), isolated from leaves of Callicarpa macrophylla, produced significantly higher growth and multiplication of in vitro shoot cultures of Rauwolfia serpentina at 0.25 and 0.5 mg/L concentrations, respectively, compared to certain other plant growth regulators (0.1-5.0 mg/L) tested under in vitro conditions. This is the first report of the plant growth promoting activities of 1 and 2 in plant tissue cultures.
GasperT, KeversC, PenelC, GreppinH, ReidDM, ThorpeTA. (1996) Plant hormones and plant growth regulators in plant tissue culture. In Vitro Cellular and Developmental Biology-Plant, 33, 272–289.
2.
SenguptaP, SenM, PalBC, PakrashiSC, AliE. (1976) Chemical constituents of the leaves of Callicarpa macrophylla Vahl. Journal of Indian Chemical Society, LIII, 218–219.
3.
OrtegaRC, CorderoGA, AnayaAL. (2002) Allelochemical stress produced by aqueous lechates of Callicarpa accuminata: effects on roots of bean, maize and tomato. Physiologia Plantarum, 116, 20–27.
4.
AnayaAL, MataR, SimsJJ, ColomaAG, OrtegoRC, GuadanoA, BautistaBEF, MidlandSL, RiosG, PompaAG. (2003) Allelochemical potential of Callicarpa accuminata.Journal of Chemical Ecology, 29, 2761–2776.
5.
ChatterjeeA, DeshmukhSK, ChandrasekharanS. (1972) Diterpenoid constituents of Callicarpa macrophylla Vahl. The structure and stereochemistry of calliterpenone and calliterpenone monoacetate. Tetrahedron, 28, 4319–4323.
6.
FujitaE, OchiaiM, IchidaI, ChatterjeeA, DesmukhSK. (1975) Confirmation of the structure of calliterpenone, a diterpenone from Callicarpa macrophylla.Phytochemistry, 14, 2249–2251.
7.
SinghAK, AgrawalPK. (1994) 3β, 16α, 17-Trihydroxyphyllcladane, a diterpenoid obtained after microbial transformation of the aqueous extract of Callicarpa macrophylla. Indian Journal of Chemistry, 33B, 1205–1206.
8.
SinghAK, AgrawalPK. (1994) 16α,17-Isopropylideno-3-oxophyllocladane, a diterpenoid from Callicarpa macrophylla. Phytochemistry, 37B, 587–588.
9.
AgrawalPK, BishnoiV, SinghAK. (1995) NMR chemical shift correlations in 16, 17-dihydroxy kaurenoids implication for stereochemical assignments. Phytochemistry, 39, 929–930.
10.
AgrawalPK, SinghAK, BhakuniRS. (1996) Complete spectral assignment of calliterpenone by two-dimensional NMR technique and carbon-13 NMR assignment for related phyllocladane diterpenoid. Indian Journal of Chemistry, 35B, 803–805.
11.
HansonJR, WhiteAF. (1969) Terpenoid biosynthesis IV. Biosynthesis of the kaurenolids and gibberellic acid. Journal of Chemical Society C, 981–985.
12.
LiuG, MullerR, RuediP. (2003) Chemical transformation of phyllocladane (=13β-kaurane) diterpenoids. Helvetica Chimica Acta, 86, 420–438.
13.
BottiniR, CassanF, PiccoliP. (2004) Gibberellin production by bacteria and its involvement in plant growth promotion and yield increase. Applied Microbiology and Botechnology, 65, 497–503.
14.
SinghAK, BagchiGD, SinghS, DwivediPD, GuptaAK, KhanujaSPS. (2004) Use of phyllocladane diterpenoids for plant growth promotion and alleviation of growth retardant alllochemicals and method there of. US Patent-6,673,749, 2004; GB 2411651, 2005.
15.
SinghAK, KhanujaSPS, TendonS, KalraA, SahooD, KaholAP, GuptaMM, VermaRK, KukrejaAK, AlamM, BagchiGD, BansalRP, DarokarMP, GuptaAK. (2005) Improved process for isolation of calliterpenone, a natural plant growth promoting phyllocladane diterpenoid from genus Callicarpa. Filed in US PCT, India 0114 NF/2005. PCT/1B 2006/002302t.
16.
GrayWM. (2004) Hormonal regulation of plant growth and development. PLoS Biology, 2, 1270–1273.
17.
GomiK, MatsuokaM. (2003) Gibberellin signaling pathway. Current Opinion in Plant Biology, 6, 489–493.
18.
RojaPC, SipahimaliniAT, HebleMR, ChadhaMS. (1987) Multiple shoot cultures of Rauwolfia serpentina: Growth and alkaloid productionJournal of Natural Products, 50, 872–875.
19.
SarkarKP, IslamA, IslamR, HoqueA, JorarderOI. (1996) In vitro propagation of Rauwolfia serpentina through tissue culture. Planta Medica, 62, 358–359.
20.
GoelMK, KukrejaAK, KhanujaSPS. (2007) Cost effective approaches for in vitro mass propagation of Rauwolfia serpentina Benth. ex. Kurz., Asian Journal of Plant Sciences, in press.
21.
MurashigeT, SkoogF. (1962) A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiologia Plantarum, 15, 473–497.
