Using appropriate isolation and fractionation procedure, and UHPLC-MS/MS as powerful analytical tool, comprehensive phenolic acid profile of leaves and flowers of Hypericum perforatum L., growing wild in Bosnia and Herzegovina, has been analyzed for the first time. The phenolic acids were isolated from plant tissue, liberated from esters and glycosidic bonds, and then analyzed as free acids. Results show that free forms of caffeic and chlorogenic acid were the most abundant. Generally, the content of acids is higher in flowers than in leaves, but environmental factors do not significantly influence chemical composition of this medicinal plant.
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