Apart from its regulatory function in lipid and glucose metabolism, peroxisome proliferator-activated receptor (PPAR)γ has impact on the regulation of inflammation and bone metabolism. The aim of the study was to investigate the association of five polymorphisms (rs10865710, rs2067819, rs3892175, rs1801282, rs3856806) within the PPARG gene with chronic periodontitis. The study population comprised 402 periodontitis patients and 793 healthy individuals. Genotyping of the PPARG gene polymorphisms was performed by PCR and melting curve analysis. Comparison of frequency distribution of genotypes between individuals with periodontal disease and healthy controls for the polymorphism rs3856806 showed a P-value of 0.04 but failed to reach significance after correction for multiple testing (P < 0.01). Two single nucleotide polymorphisms (SNPs) (rs10865710 and rs3892175) were found to be in strong linkage disequilibrium to rs2067819 (D’ > 0.90). A 3-site analysis (rs2067819-rs1801282-rs3856860) revealed five haplotypes with a frequency of ≥1% among cases and controls. Following adjustment for age, gender and smoking, none of the haplotypes was significantly different between periodontitis and healthy controls after Bonferroni correction. This study could not show a significant association between PPARG gene variants and chronic periodontitis.
PageRC. Milestones in periodontal research and the remaining critical issues. J Periodont Res1999; 34: 331–339.
2.
GravesDJiangYGencoC. Periodontal disease: bacterial virulence factors, host response and impact on systemic health. Curr Opin Infect Dis2000; 13: 227–232.
3.
MichalowiczBSDiehlSRGunsolleyJCSparksBSBrooksCHKoertgeTE. Evidence of a substantial genetic basis for risk of adult periodontitis. J Periodontol2000; 71: 1699–1707.
4.
KinaneDFDemuthDRGorrSUHajishengallisGNMartinMH. Human variability in innate immunity. Periodontol 20002007; 45: 14–34.
5.
ShapiraLWilenskyAKinaneDF. Effect of genetic variability on the inflammatory response to periodontal infection. J Clin Periodontol2005; 32(Suppl): 72–86.
6.
YoshieHKobayashiTTaiHGaliciaJC. The role of genetic polymorphisms in periodontitis. Periodontol 20002007; 43: 102–132.
7.
RizzoGFiorucciS. PPARs and other nuclear receptors in inflammation. Curr Opin Pharmacol2006; 6: 421–427.
8.
LehmannJMMooreLBSmith-OliverTAWilkisonWOWillsonTMKliewerSA. An antidiabetic thiazolidinedione is a high affinity ligand for peroxisome proliferator-activated receptor gamma (PPAR gamma). J Biol Chem1995; 270: 12953–12956.
9.
FormanBMChenJEvansRM. Hypolipidemic drugs, polyunsaturated fatty acids, and eicosanoids are ligands for peroxisome proliferator-activated receptors alpha and delta. Proc Natl Acad Sci USA1997; 94: 4312–4317.
10.
DesvergneBWahliW. Peroxisome proliferator-activated receptors: nuclear control of metabolism. Endocr Rev1999; 20: 649–688.
11.
CuzzocreaSPisanoBDugoLIanaroAMaffiaPPatelNS. Rosiglitanzone, a ligand of the peroxisome proliferator-activated receptor-gamma, reduces acute inflammation. Eur J Pharmacol2004; 483: 79–93.
12.
JiangCTingATSeedB. PPAR-gamma agonists inhibit production of monocyte inflammatory cytokines. Nature1998; 391: 82–86.
13.
TontonozPNagyLAlvarezJGThomazyVAEvansRM. PPARgamma promotes monocyte/macrophage differentiation and uptake of oxidized LDL. Cell1998; 93: 241–252.
14.
RicoteMLiACWillsonTMKellyCJGlassCK. The peroxisome proliferator-activated receptor-gamma is a negative regulator of macrophage activation. Nature1998; 391: 79–82.
15.
MeirhaegheAFajasLGouilleuxFCottelDHelbecqueNAuwerxJ. A functional polymorphism in a STAT5B site of the human PPARg3 gene promoter affects height and lipid metabolism in a French population. Arterioscler Thromb Vasc Biol2003; 23: 289–294.
16.
MeirhaegheAAmouyelP. Impact of genetic variation of PPARgamma in humans. Mol Genet Metab2004; 83: 93–102.
17.
SugawaraKOlsonTSMoskalukCAStevensBKHoangSKozaiwaK. Linkage to peroxisome proliferator-activated receptor-gamma in SAMP1/YitFc mice and in human Crohn’s disease. Gastroenterology2005; 128: 351–360.
18.
RidkerPMCookNRChengSErlichHALindpaintnerKPlutzkyJ. Alanine for proline substitution in the peroxisome proliferator-activated receptor gamma-2 (PPARG2) gene and the risk of incident myocardial infarction. Arterioscler Thromb Vasc Biol2003; 23: 859–863.
IzumiKTobitaTFeinbergSE. Isolation of human oral keratinocyte progenitor/stem cells. J Dent Res2007; 86: 341–346.
30.
Di PaolaRMazzonEMaiereDZitoDBrittiDDe MajoM. Rosiglitanzone reduces the evolution of experimental periodontitis in the rat. J Dent Res2006; 85: 156–161.
31.
HassumiMYSilva-FilhoVJCampos-JúniorJCVieiraSMCunhaFQAlvesPM. PPAR-gamma agonist rosiglitazone prevents inflammatory periodontal bone loss by inhibiting osteoclastogenesis. Int Immunopharmacol2009; 9: 1150–1158.
32.
HiranoESugitaNKikuchiAShimadaYSasaharaJIwanagaR. Peroxisome proliferator-activated receptor gamma polymorphism and periodontitis in pregnant Japanese women. J Periodontol2010; 81: 897–906.
33.
NibaliLDonosNHendersonB. Periodontal infectogenomics. J Med Microbiol2009; 58: 1269–12674.
