Apigeninidin-enriched Sorghum bicolor (L. Moench) extracts alleviate Aflatoxin B 1 -induced dysregulation of male rat hypothalamic-reproductive axis

Abstract

We examined the protective effect of the apigeninidin (API)-enriched fraction from Sorghum bicolor sheaths extracts (SBE-05, SBE-06, and SBE-07) against aflatoxin B₁ (AFB₁)-induced dysregulation of male rat’s reproductive system that may trigger infertility. Male rats (160 ± 12 g) were treated with AFB₁ (50 µg/kg) along with 5 or 10 mg/kg of SBE-05, SBE-06, and SBE-07 for 28 days. Subsequently, we assessed the reproductive hormone—prolactin, FSH, LH, testosterone levels, and testicular function enzymes. Moreover, we examined rats’ testes, epididymis, and hypothalamus for oxidative and inflammatory stress biomarkers, caspase-9 activity and tissues pathology. We observed that comparative to AFB₁ alone treated rats, API co-treatment significantly (p < 0.05) abated the AFB₁-mediated decrease in prolactin and antioxidant defenses and lessened lipid peroxidation (LPO) and reactive oxygen and nitrogen species levels in the examined organs—testes, epididymis, and hypothalamus. API abated AFB₁-induced hormone decreases—testosterone, FSH, and LH; and caused improvement in sperm quantity and quality. API lessened AFB₁-mediated increase in pro-inflammatory cytokine, increased interleukin-10 level, an anti-inflammatory cytokine and reduced caspase-9 activities. In addition, API reduced alterations in the examined tissue histology. Our findings suggest that S. bicolor API-enrich extracts have active antioxidative, antiapoptotic, and anti-inflammatory activities, which can protect against AFB₁-induced dysfunction of the hypothalamic–pituitary–gonadal axis.