This study investigated the relationship between mast cell numbers and the grade of bladder transitional cell carcinoma (TCC). Bladder TCC biopsies were obtained via transurethral resection and 45 stage T1 specimens were included in the study. Specimens were sorted into two groups, low grade (grade I) and high grade (grades II and III). Samples were stained using haematoxylin and eosin, toluidine blue and immunohistochemical staining for tryptase. Mast cells were examined by light microscopy and cell density was recorded. Mast cell density was significantly higher in high-grade TCC than low-grade TCC. There was also a significant relationship between the number of mast cells identified using toluidine blue staining or immunohistochemical staining for tryptase. Detailed studies of mast cell function will enable the development of more effective antitumour therapies via mast cell manipulation.
TheoharidesTCContiP: Mast cells: the Jekyll and Hyde of tumor growth. Trends Immunol2004; 25: 235–241.
2.
ShakedYKerbelRS: Antiangiogenic strategies on defense: on the possibility of blocking rebounds by the tumor vasculature after chemotherapy. Cancer Res2007; 67: 7055–7058.
3.
GalliSJNakaeSTsaiM: Mast cells in the development of adaptive immune responses. Nat Immunol2005; 6: 135–142.
4.
KobayashiHIshizukaTOkayamaY: Human mast cells and basophils as sources of cytokines. Clin Exp Allergy2000; 30: 1205–1212.
5.
PlautMPierceJHWatsonCJ: Mast cell lines produce lymphokines in response to cross-linkage of FcεRI or to calcium ionophores. Nature1989; 339: 64–67.
6.
HultnerLDruezCMoellerJ: Mast cell growth-enhancing activity (MEA) is structurally related and functionally identical to the novel mouse T cell growth factor P40/TCGFIII (interleukin 9). Eur J Immunol1990; 20: 1413–1416.
7.
GalinskyDSNechushtanH: Mast cells and cancer—no longer just basic science. Crit Rev Oncol Hematol. 2008; 68: 115–130.
8.
FisherERPaikSMRocketteH: Prognostic significance of eosinophils and mast cells in rectal cancer: findings from the National Surgical Adjuvant Breast and Bowel Project (protocol R-01). Hum Pathol1989; 20: 159–163.
9.
RibattiDVaccaARiaR: Neovascularisation, expression of fibroblast growth factor-2, and mast cells with tryptase activity increase simultaneously with pathological progression in human malignant melanoma. Eur J Cancer2003; 39: 666–674.
10.
ImadaAShijuboNKojimaH: Mast cells correlate with angiogenesis and poor outcome in stage I lung adenocarcinoma. Eur Respir J2000; 15: 1087–1093.
11.
RibattiDVaccaANicoB: Bone marrow angiogenesis and mast cell density increase simultaneously with progression of human multiple myeloma. Br J Cancer1999; 79: 451–455.
12.
MolinDEdstromAGlimeliusI: Mast cell infiltration correlates with poor prognosis in Hodgkin's lymphoma. Br J Haematol2002; 119: 122–124.
13.
RibattiDFinatoNCrivellatoE: Neovascularization and mast cells with tryptase activity increase simultaneously with pathologic progression in human endometrial cancer. Am J Obstet Gynecol2005; 193: 1961–1965.
14.
FisherERSassRWatkinsG: Tissue mast cells in breast cancer. Breast Cancer Res Treat1985; 5: 285–291.
15.
DabiriSHuntsmanDMakretsovN: The presence of stromal mast cells identifies a subset of invasive breast cancers with a favorable prognosis. Mod Pathol2004; 17: 690–695.
16.
WelshTJGreenRHRichardsonD: Macrophage and mast-cell invasion of tumor cell islets confers a marked survival advantage in non-small-cell lung cancer. J Clin Oncol2005; 23: 8959–8967.
17.
ChanJKMagistrisALoizziV: Mast cell density, angiogenesis, blood clotting, and prognosis in women with advanced ovarian cancer. Gynecol Oncol2005; 99: 20–25.
18.
TunaBYorukogluKUnluM: Association of mast cells with microvessel density in renal cell carcinomas. Eur Urol2006; 50: 530–534.
19.
SamoszukMCorwinMA: Acceleration of tumor growth and peritumoral blood clotting by imatinib mesylate (Gleevec™). Int J Cancer2003; 106: 647–652.
20.
SariASerelTACandirO: Mast cell variations in tumour tissue and with histopathological grading in specimens of prostatic carcinoma. BJU Int1999; 84: 851–853.
21.
GuptaRK: Mast cell variations in prostate and urinary bladder. Arch Pathol1970; 89: 302–305.
22.
SerelTASoyupekSCandirO: Association between mast cells and bladder carcinoma. Urol Int2004; 72: 299–302.
23.
WallsAFJonesDBWilliamsJH: Immunohistochemical identification of mast cells in formaldehyde-fixed tissue using monoclonal antibodies specific for tryptase. J Pathol1990; 162: 119–126.
24.
EpsteinJIAminMBReuterVR: The World Health Organization/International Society of Urological Pathology consensus classification of urothelial (transitional cell) neoplasms of the urinary bladder. Am J Surg Pathol1998; 22: 1435–1448.
