We examined the expression of CD44 variant forms and their binding to hyaluronan (HA) in lung cancer cell lines. There was no relationship between the level of expression of CD44 variants and HA binding in different lung cancer cell lines. The expression of CD44v6 and CD44E in some cell lines was not always associated with HA binding. There was no relationship between the tissue pathological type and CD44 expression or HA binding. Deglycosylation by neuraminidase induced CD44-HA binding in human lung cancer cell lines. Our findings suggest that the HA binding ability of CD44, which is negatively regulated by glycosylation, might be a more important factor in tumorigenesis or metastasis than the expression of CD44 variant forms.
BartolazziAPeachRAruffoAStamenkovicI: Interaction between CD44 and hyaluronate is directly implicated in the regulation of tumor development. J Exp Med1994; 180: 53–66
2.
PickerLJDe los ToyosJTelenMJ: Monoclonal antibodies against the CD44 [In(Lu)-related p80], and Pgp-1 antigens in man recognize the Hermes class of lymphocyte homing receptors. J Immunol1989; 142: 2046–2051
3.
LesleyJHymanRKincadePW: CD44 and its interaction with the extracellular matrix. Adv Immunol1993; 54: 271–335
4.
DeGrendeleHCKosfiszerMEstessP: CD44 activation and associated primary adhesion is inducible via T cell receptor stimulation. J Immunol1997; 159: 2549–2553
5.
HathcockKSHiranoHMurakamiS: CD44 expression on activated B cells. Differential capacity for CD-44 dependent binding to hyaluronic acid. J Immunol1993; 151: 6712–6722
6.
MollJSchmidtAvan der PuttenH: Accelerated immune response in transgenic mice expressing rat CD44v4v7 on T Cells. J Immunol1996; 156: 2085–2094
7.
ScreatonGRBellMVJacksonDG: Genomic structure of DNA encoding the lymphocyte homing receptor CD44 reveals at least 12 alternatively spliced exons. Proc Natl Acad Sci USA1992; 89: 12160–12164
8.
TakeuchiKYamaguchiAUranoT: Expression of CD44 variant exons 8–10 in colorectal cancer and its relationship to metastasis. Jpn J Cancer Res1995; 86: 292–297
9.
YamaguchiASaitoMGoiT: Expression of CD44 variant exons 8–10 in gastric cancer. Jpn J Cancer Res1995; 86: 1166–1171
10.
TokueYMatsumuraYKatsumataN: CD44 variant isoform expression and breast cancer prognosis. Jpn J Cancer Res1998; 89: 283–290
11.
CannistraSADeFranzoBNiloffJ: Functional heterogeneity of CD44 molecules in ovarian cancer cell lines. Clin Cancer Res1995; 1: 333–342
12.
TakigawaNSegawaYMandaiK: Serum CD44 levels in patients with non-small cell lung cancer and their relationship with clinicopathological features. Lung Cancer1997; 18: 147–157
13.
WashimiOUedaRAriyoshiY: Expression of CD44 variant isoforms in normal and neoplastic cells of the lung. Jpn J Cancer Res1994; 85: 1112–1116
14.
WimmelASchilliMKaiserU: Preferential histiotypic expression of CD44 isoforms in human lung cancer. Lung Cancer1997; 16: 151–172
15.
BennettKLModrellBGreenfieldB: Regulation of CD44 binding to hyaluronan by glycosylation of variably spliced exons. J Cell Biol1995; 131: 1623–1633
16.
KatohSZhengZOritaniK: Glycosylation of CD44 negatively regulates its recognition of hyaluronan. J Exp Med1995; 182: 419–429
17.
SaegusaMOkayasuI: Up-regulation of CD44 variant exon expression in endometrial carcinomas: analysis of mRNA and protein isoforms, and relation to clinicopathological factors. Jpn J Cancer Res1998; 89: 291–298
18.
JunZHillPALanHY: CD44 and hyaluronan expression in the development of experimental crescentic glomerulonephritis. Clin Exp Immunol1997; 108: 69–77
19.
LiaoHXLeeDMLevesqueMC: N-terminal and central regions of the human CD44 extracellular domain participate in cell surface hyaluronan binding. J Immunol1995; 155: 3938–3945
20.
PerschlALesleyJEnglishN: Role of CD44 cytoplasmic domain in hyaluronan binding. Eur J Immunol1995; 25: 495–501
21.
PureECampRLPerittD: Defective phosphorylation and hyaluronate binding of CD44 with point mutations in the cytoplasmic domain. J Exp Med1995; 181: 55–62
22.
TakahashiKStamenkovicICutlerM: Keratan sulfate modification of CD44 modulates adhesion to hyaluronate. J Biol Chem1996; 271: 9490–9496
23.
LesleyJEnglishNPerschlA: Variant cell lines selected for alterations in the function of the hyaluronan recepter CD44 show differences in glycosylation. J Exp Med1995; 182: 431–437
24.
TimothyPSZengCNocksA: Glycosylation provides both stimulatory and inhibitory effects on cell surface and soluble CD44 binding to hyaluronan. J Cell Biol1998; 140: 431–446
25.
ZhengZCummingsRDPummillPE: Growth as a solid tumor or reduced glucose concentrations in culture reversibly induce CD44-mediated hyaluronan recognition by chinese hamster ovary cells. J Clin Invest1997; 100: 1217–1229
