Development of Binding Assays for the SH2 Domain of Grb7 and Grb2 Using Fluorescence Polarization

Abstract

Adaptor proteins Grb7 and Grb2 have been implicated as being 2 potential therapeutic targets in several human cancers, especially those that overexpress ErbB2. These 2 proteins contain both a SH2 domain (Src homology 2) that binds to phosphorylated tyrosine residues contained within ErbB2 and other specific protein targets. Two assays based on enzyme-linked immunosorbent assay and fluorescence polarization methods have been developed and validated to find and rank inhibitors for both proteins binding to the pY¹¹³⁹. Fluorescence polarization assays allowed the authors to determine quickly and reproducibly affinities of peptides from low nanomolar to high micromolar range and to compare them directly for Grb7 and Grb2. As a result, the assays have identified a known peptidomimetic Grb2 SH2 inhibitor (mAZ-pTyr-(αMe)pTyr-Asn-NH₂) that exhibits the most potent affinity for the Grb7 SH2 domain described to date. (Journal of Biomolecular Screening 2008:112-119)

Keywords

fluorescence polarization assay development Grb2 Grb7 SH2 domain inhibitors

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