Efecto del uso combinado de la Pronasa E y de un extracto fúngico (Mucor racemosus forma sphaerosporus) en la maduración de embutidos crudos curados / Effect of the combined use of Pronase E and a fungal extract (Mucor racemosus forma sphaerosporus) on the ripening of dry fermented sausages
Restricted accessResearch articleFirst published online August, 1999
Efecto del uso combinado de la Pronasa E y de un extracto fúngico (Mucor racemosus forma sphaerosporus) en la maduración de embutidos crudos curados / Effect of the combined use of Pronase E and a fungal extract (Mucor racemosus forma sphaerosporus) on the ripening of dry fermented sausages
The effect of the addition of 600 units of Pronase E/kg of sausage mixture and/or a fungal extract ( Mucor racemosus forma sphaerosporus) at two different concentrations (1.75 and 17.5 mg protein/kg sausage mixture) on dry fermented sausages was evaluated. The only addition of protease to sau sages led to an increase in the content of free amino acids and biogenic amines. When the protease and the fungal extract were added together at high concentration (17.5 mg protein/kg sausage mix ture), a decrease in the free amino acid content and a slight increase in the ammonia content were observed, together with a clear increase in the concentration of several volatile compounds, such as 2- and 3-methylbutanal, 2-methylpropanal and 2- and 3-methyl-1-butanol. The addition of the fungal extract only at high concentration (17.5 mg protein/kg sausage mixture) or the combined protease and fungal extract at low concentration (1.75 mg protein/kg sausage mixture), produced slight physi cochemical modifications compared with the corresponding controls. All sausages with Pronase E showed a clear modification of texture, with a marked decrease of hardness, adhesiveness, springi ness, cohesiveness, gumminess, chewiness, cutting force and cutting work. Sensory analysis of the different batches of sausages showed that the combined addition of Pronase E (600 units/kg of sau sage mixture) and the Mucor racemosus forma sphaerosporus extract at high concentration (17.5 mg protein/kg of sausage mixture) improved the odor, taste, texture and, in consequence, the global acceptability in relation to the control batch.
Barbieri G., Bolzoni L., Parolari G., Virgilli R., Buttini R., Careri M. y Mangia A. (1992). Flavor compounds of dry-cured ham. Journal of Agriculture and Food Chemistry40: 2389-2394.
2.
Berdagué J.L., Denoyer C., Le Quere J.L. y Semon E. (1991). Volatile components of dry-cured ham. Journal of Agriculture and Food Chemistry39: 1257-1261.
3.
Berdagué J.L., Monteil P., Montel M.C. y Talon R. (1993). Effects of starter cultures on the formation of flavour compounds in dry sausage. Meat Science35: 275-287.
4.
Blom H., Hagen B.F., Pedersen B.O., Holck A.L., Axelsson, L. y Naes H. (1996). Accelerated production of dry fermented sausage . Meat Science43: 229-242.
5.
Croizet F., Denoyer C., Tran N. y Berdagué J.L. (1992). Les composés volatils du saucisson sec. Evolution au cours de la maturation. Viandes et Produits Carnés13: 167-170.
6.
Demeyer D.L., Hoozee, J. y Mesdom H. (1974). Specificity of lipolysis during dry fermented sausage ripening. Journal of Food Science39: 293-296.
7.
Demeyer D.I. (1982). Stoichiometry of dry sausage fermentation. Antoine van Leewenhoek48: 414-416.
8.
Diaz O., Fernández M., Garcia de Fernando G.D., Hoz L. y Ordóñez J.A. (1993). Effect of the addition of Pronase E on the proteolysis of dry fermented sausages. Meat Science34: 205-216.
9.
Díaz O., Fernández M., Garcia de Fernando G.D., Hoz L. y Ordóñez J.A. (1996). Effect of the addition of papain on the dry fermented sausage proteolysis. Journal of the Science of Food and Agriculture71: 13-21.
10.
Diaz O., Fernández M., Garcia de Fernando G.D., Hoz L. y Ordóñez J.A. (1997). Proteolysis in dry fermented sausages: the effect of selected exogenous proteases. Meat Science46: 115-128
11.
Domínguez M.C. y Zumalacárregui J.M. (1991). Lipolytic and oxidative changes in "chorizo" during ripening. Meat Science29: 99-107.
12.
Edwards R.A., Ordóñez J.A., Dainty R.H., Hierro E.M. y Hoz L. (1999). Characterization of the headspace volatile compounds of selected Spanish dry fermented sausages. Food Chemistry64: 461-465.
13.
Fernández M., Hoz L., Diaz O., Cambero I. y Ordóñez J.A. (1995a). Effect of the addition of pancreatic lipase on the ripening of dry fermented sausages. Part I. Microbial, physicochemical and lipolitic changes. Meat Science40: 159-170.
14.
Fernández M., Hoz L., Diaz O., Cambero I. y Ordóñez J.A. (1995b). Effect of the addition of pancreatic lipase on the ripening of dry fermented sausages. Part II. Free fatty acids, short chain fatty acids, carbonyls and sensorial quality. Meat Science40: 351-362.
15.
Garcia C., Berdagué J.J., Antequera T., López-Bote C., Córdoba J.J. y Ventanas J. (1991). Volatile components of dry cured Iberian ham. Food Chemistry41: 23-32.
16.
Halász A., Baráth A., Simo-Sarkadi L. y Hozapfel W. (1994). Biogenic amines and their production by microorganisms in food. Trends in Food Science and Technology5: 42-49.
17.
Hanson S.W.F. y Olley J. (1963). Application of the Bligh and Dyer method of lipid extraction to tissue homogenates. Biochemical Journal89: 351-362.
18.
Hinrichsen L.L. y Andersen H.J. (1994). Volatile compounds and chemical changes in cured pork: role of three halotolerant bacteria. Journal of Agriculture and Food Chemistry42: 1537-1542.
Kandler O. (1983). Carbohydrate metabolism in lactic acid bacteria . Antoine van Leeuwenhoek49: 209-224.
21.
Kinsella S.E. y Hwang D. (1976). Biosynthesis of flavors by Penicillium roqueforti . Biotechnology and Bioengineering18: 927-938.
22.
León Crespo F., Barranco A., Penedo J.C., Beltrán de Heredia F., Mata C., Montero E. y Martins C. (1985). Proteolisis y lipolisis en la maduración del chorizo . Alimentaria163: 51-53.
23.
Lowry O.H., Rosenbrough N.J., Farr A.L. y Randall R.J. (1951). Protein measurement with the pholin phenol reagent . Journal of Biological Chemistry193: 265-275.
Melendo J.A., Beltrán J.A., Jaime I., Sancho R. y Roncalés, P. (1996). Limited proteolysis of myofibrilar proteins by bromelain decreases toughness of coarse dry sausage. Food Chemistry57: 429-433.
26.
Naes H., Holck A.L., Axelsson L., Andersen H.J. y Blom H. (1995). Accelerated ripening of dry fermented sausage by addition of a Lactobacillus proteinase. International Journal Food Science and Technology29: 651-659.
27.
Nychas G.J.E. y Arkoudelos J.S. (1990). Staphylococci: their role in fermented sausages . Journal of Applied Bacteriology Symposium Supplement: 167S-188S.
28.
Ordóñez J.A., Asensio M.A., Garcia M.L., Selgas M.D. y Sanz B. (1989). A reasonably aseptic method for monitoring the fenomena ocurring during the ripening of fermented sausages. Fleischwirtschaft69: 1023-1025.
29.
Ordóñez J.A., de Pablo B., Pérez de Castro B., Asensio M.A. y Sanz B. (1991). Selected chemical and microbiological changes in refrigerated pork stored in carbon dioxide and oxygen enriched atmospheres . Journal of Agriculture and Food Chemistry39: 668-672.
30.
Renner E. (1987). Nutritional aspects of cheese. En: Fox PE.(ed.) Cheese: Chemistry, Physics and Microbiology I. General aspects. London: Elsevier Applied Science. pp. 345-363.
31.
Sanz B., Selgas D., Parejo I. y Ordóñez J.A. (1988). Characteristics of lactobacilli isolated from dry fermented sausages. International Journal of Food Microbiology6: 199-205.
32.
Toledo V.M., Selgas M.D., Casas M.C., Ordóñez J.A. y Garcia M.L. (1997). Effect of selected mould strains on proteolysis in dry fermented sausages. Zeitschrift für Lebensmittel Untersuchung und Forschung204: 385-390.
33.
Trigueros G., Garcia M.L., Casas C., Ordóñez J.A. y Selgas M.D. (1995). Proteolytic and lipolytic activities of mould strains isolated from Spanish dry fermented sausages. Zeitschrift für Lebensmittel Untersuchung und Forschung201: 298-302.
34.
Wellner D. y Lichtenberg L.A. (1971). Assay of amino acid oxidase. En: Tabor H. y White C. (eds.). Methods in Enzymology. Vol. XVII Parte B. London: Academic Press. pp. 593-596.
35.
Zalacain I., Zapelena M.J., Astiasarán, I. y Bello J. (1995). Dry fermented sausages elaborated with lipase from Candida cylindracea. Comparison with traditional formulations. Meat Science40: 55-61.
36.
Zalacain I., Zapelena M.J., Astiasarán I. y Bello J. (1996). Addition of lipase from Candida cylindracea to a traditional formulation of a dry fermented sausage. Meat Science42: 155-163.
37.
Zalacain I., Zapelena M.J., Paz de Peña M., Astiasarán I. y Bello J. (1997a). Use of lipase from Rhizomucor miehei in dry fermented sausages elaboration: microbial, chemical and sensory analisis. Meat Science45: 99-105.
38.
Zalacain I., Zapelena M.J., Paz de Peña M., Astiasarán I. y Bello, J. (1997b). Application of Lipozyme 10,000 L (from Rhizomucor miehei) in dry fermented sausage technology: study in a pilot plant and at the industrial level. Journal of Agricultural and Food Chemistry45: 1972-1976.
39.
Zapelena M.J., Zalacain I., Paz de Peña M., Astiasarán I. y Bello J. (1997). Addition of a neutral proteinase from Bacillus subtilis (Neutrase) together with a starter to a dry fermented sausage elaboration and its effect on the amino acid profiles and the flavor development. Journal of Agricultural and Food Chemistry45: 472-475.
