Two batches of dry-cured fermented sausage were made by industrial methods at pilot scale. The difference between them was the drying temperature applied (5 or 12 °C) for a period of time extending from the moment when pH = 5 was attained until a weight loss of 20% was achieved. The other conditions were the same for the two processes. Changes in pH, total acidity, D and L-lactic acids, acetic acid, total volatile basic nitrogen (TVBN), free amino acids (FAA) and free fatty acids (FFA) were studied, and a discriminatory sensory analysis of the ready-to-eat product was carried out. Only L-lactic acid and total acidity within the acidity parameters showed substantial differences halfway through the drying process. No significant differences were found in any of the acidity variables at the end of the process. The drying temperature encouraged the production of TVBN, but no direct relationship was established between it and the quantity of FAA. The three fractions of FFA [SFA (saturated fatty acids), MUFA (monounsaturated fatty acids) and PUFA (polyunsaturated fatty acids)] increased during the curing period, always higher at 12 °C than at 5 °C throughout the process. No differences between the two processes were detected by the discriminatory sensory analysis.
AICE. (2000). Panorama del sector cárnico.http://www.aice.es (Visited February 2000).
2.
Aristoy M.C. and Toldrá F. (1991). Deproteinization techniques for HPLC amino acids analysis in fresh pork muscle and dry-cured ham. Journal of Agriculture and Food Chemistry39: 1972–1975.
3.
Berry J.F., Cevallos W.H. and Wade R.R. (1965). Lipid class and fatty acids composition of intact peripheral nerve and during wallerian degeneration. Journal of American Oil Chemical Society42: 492–495.
4.
Bidlingmeyer B.A., Cohen S.A., Tarvin T.L. and Frost B. (1987). A new, rapid, high-sensitivity analysis of amino acids in food type samples. Journal of the Association of Official Analytical Chemists70: 241–247.
5.
Blom H., Hagen B.F., Pedersen B.O., Holck A.L., Axelsson L. and Naes H. (1996). Accelerated production of dry fermented sausage. Meat Science43: 229–242.
6.
Boehringer Mannheim. (1999). Methods of Enzymatic Bioanalysis and Food Analysis. Mannheim, Germany: Boehringer Mannheim, pp. 6–9, 74–77.
7.
Centre Technique de la Salaison Charcuterie et Conserves de Viandes. (1980). Code de la Charcuterie des Conserves de Viandes. Méthodes de Contrôle. Maisons-Alfor, France: Centre Technique de la Salaison Charcuterie et Conserves de Viandes, pp. 37–39.
8.
DeMasi T., Warlaw F.B., Dick R.L. and Acton J.C. (1990). Nonprotein nitrogen (NPN) and free amino acids contents of dry fermented and nonfermented sausages. Meat Science27: 1–12.
9.
Díaz O., Fernández M., García de Fernando G.D., De la Hoz L. and Ordóñez J.A. (1993). Effect of the addition of Pronase E on the proteolysis in dry fermented sausages. Meat Science34: 205–216.
10.
Díaz O., Fernández M., García de Fernando G.D., De la Hoz L. and Ordóñez J.A. (1996). Effect of the addition of Papain on the dry fermented sausages proteolysis. Journal of the Science of Food and Agriculture71: 13–21.
11.
Domínguez M.C. and Zumalacarregui J.M. (1991). Lipolytic and oxidative changes in “chorizo” during ripeneing. Meat Science29: 99–107.
12.
Flores J. and Bermell S. (1996). Dry-cured sausages. Factors influencing souring and their consequences. Fleischwirtschaft76: 163–165
13.
Flores M., Aristoy M.C., Spanier A.M. and Toldrá F. (1997). Non-volatile components effects on quality of “Serrano” dry cured ham as related to processing time. Journal of Food Science62: 1235–1239.
14.
Flores, M., Moya V-J., Aristoy M-C. and Toldrá F. (2000). Nitrogen compounds as potential biochemical markers of pork meat quality. Food Chemistry69: 371–378.
15.
Folch J., Lees M. and Sloane-Stanley G.H.S. (1957). A simple method of isolation and purification of total lipids from animal tissues. Journal of Biological Chemistry26: 497–507.
16.
Garriga M., Compte M., Casademont G. and Moreno-Amich R. (1988) Influencia de los carbohidratos en el proceso de fermentación del salchichón. Revista de Agroquímica y Tecnología de Alimentos28: 548–557.
17.
Incze K. (1992). Raw fermented and dried meat products. Fleischwirtschaft72: 58–62.
18.
ISO. (1973). Determinación de humedad en carne y productos cárnicos. ISO 1442-1973(E). ISO: Geneve.
19.
Johansson G., Berdagué J.L., Larsson M., Tran N. and Borch E. (1994). Lipolysis, proteolysis and formation of volatile components during ripening of a fermented sausage with Pediococcus pentosaceus and Staphylococcus xylosus as starter cultures. Meat Science38: 203–218.
20.
Kempton A.G. and Bobier S.R. (1970). Bacterial growth in refrigerated vacuum-packed luncheon meats. Canadian Journal of Microbiology16: 287–289.
21.
Kenneally P.M., Leuscher R.G. and Arendt E.K. (1998). Evaluation of the lipolytic activity of starter cultures for meat fermentation purposes. Journal of Applied Microbiology84: 839–846.
22.
Klettner P.G. and Baumgartner P.A. (1980). The technology of raw dry sausage manufacture. Food Technology in Australia32: 380–384.
23.
Landvogt A. and Fischer A. (1990). Rohwurstreifung. Geielte. Steuerung der Säuerungsleistung von Starter Kulturen. Fleischwirtschaft70: 1134–1140.
24.
Lois A.L., Gutierrez L.M., Zumalacarregui J.M. and Lopez A. (1987). Changes in several constituents during ripening of “chorizo” a Spanish dry sausage. Meat Science19: 169–177.
25.
Marchesini B., Bruttin A., Romailler N., Moreton R.S., Stuchi C. and Sozzi T. (1992). Microbiological events during commercial meat fermentations. Journal of Applied Bacteriology73: 203–209.
26.
Mateo J., Domínguez M.C., Aguirrezábal M.M. and Zumalacarregui J.M. (1996). Taste compounds in chorizo and their changes during ripening. Meat Science44: 245–254.
27.
Molly K., Demeyer D., Civera T. and Verplaetse A. (1996). Lipolysis in a Belgian sausage: relative importance of endogenous and bacterial enzymes. Meat Science43: 235–244.
28.
Molly K., Demeyer D., Johansson G., Raemaekers M., Ghistelinck M. and Geenen I. (1997). Importance of meat enzymes in ripening and flavour generation in dry fermented sausages. Food Chemistry59: 539–545.
29.
Montel M.C., Talon R., Berdagué J.L. and Cantonnet M. (1993). Effects of starter cultures on the biochemical characteristics of French dry sausages. Meat Science35: 229–240.
30.
Navarro J.L., Nadal M.I., Izquierdo L. and Flores J. (1997). Lipolysis in dry cured sausages as affected by processing conditions. Meat Science45: 161–168.
31.
Navarro J.L., Nadal M.I., Nieto P. and Flores J. (1998). Effect of nitrate and nitrite curing salts on lipolysis in dry sausages produced using a rapid fermentation process. Food Research and Technology206: 217–221.
32.
Needs E.C., Ford G.D., Owen A.J. and Tuckley B. (1983). A method for the quantitative determination of individual free fatty acids in milk by ion exchange resin adsorption and gasliquid chromatography. Journal of Dairy Researcy50: 321–329.
33.
Stahnke L.H. (1994). Aroma components from dried sausages fermented with Staphylococcus xylosus. Meat Science38: 39–53.
34.
Stiebing A. and Rödel W. (1990). Influence of the pH on the drying pattern in dry sausage. Fleischwirtschaft70: 1039–1043.
35.
Verplaetse A. (1994). Influence of raw meat properties and processing technology on aroma quality of raw fermented meat products. In: Proceedings of 40th International Congress of Meat Science and Technoloogy. The Hague. pp. 45–65.
36.
Zapelena M.J., Zalacain I., De Peña M.P., Astiasarán I. and Bello J. (1997). Addition of a neutral proteinase from Bacillus subtilis (Neutrase) together with a starter to a dry fermented sausage elaboration and its effect on the amino acid profiles and the flavour development. Journal of Agriculture and Food Chemistry45: 472–475.
