Salmonella spp. are one of the most common causes of foodborne disease outbreaks worldwide. Disinfectants are widely used in the food industry to reduce pathogen contamination, but the increase in antimicrobial resistance has reinforced the global need for effective and environmentally friendly alternatives. In addition, Salmonella Heidelberg, an emergent serotype, has been described as highly persistent in facilities of poultry production chain. In this context, the present study aimed to evaluate the action of electrochemically activated water (ECAW), a biocide produced from water, salt, and electricity, against aerobic bacteria, including S. Heidelberg, experimentally inoculated in chicken breasts. Chicken breasts fragments (2 g) were inoculated by immersion in the bacterial inoculum solution and then were left in a petri dish for 10 min to allow microbial attachment. Fragments were treated by immersion in ECAW (treated group) or 0.1% sterile peptone water solution (control group) at 25 °C for 10 min. After, chicken breasts slices were transferred to sterile tubes and were incubated at 7 °C and at three contact times of 5, 30, and 60 min to simulate chiller environment. The average reduction was 1.07 log10 CFU/g after treatment, and the bacterial counts decreased significantly (p < 0.05) with increasing contact time. These results demonstrate the potential use of this technology in chicken slaughter plants.
AfariGKHungYC (2018) A meta-analysis on the effectiveness of electrolyzed water treatments in reducing foodborne pathogens on different foods. Food Control93: 150–164.
2.
Al-HolyMARascoBA (2015) The bactericidal activity of acidic electrolyzed oxidizing water against Escherichia coli O157:H7, Salmonella typhimurium, and Listeria monocytogenes on raw fish, chicken and beef surfaces. Food Control54: 317–321.
3.
AraujoPCOSariMHMJardimNS, et al. (2020) Effect of m-trifluoromethyl-diphenyl diselenide on acute and subchronic animal models of inflammatory pain: Behavioral, biochemical and molecular insights. Chemico-Biological Interactions317: 108941.
4.
BorgesKAFurianTQSouzaSN, et al. (2018) Biofilm formation capacity of Salmonella serotypes at different temperature conditions. Pesquisa Veterinária Brasileira38(1): 71–76.
5.
Centers for Disease Control and Prevention (CDC). Salmonella, 2023. Available at: https://www.cdc.gov/salmonella/ (accessed: 05 March 2023)
6.
DewiGNairDVTPeichelC, et al. (2021) Effect of lemongrass essential oil against multidrug-resistant Salmonella Heidelberg and its attachment to chicken skin and meat. Poultry Science100(7): 101116.
7.
DuanDWangHXueS, et al. (2017) Application of disinfectant sprays after chilling to reduce the initial microbial load and extend the shelf-life of chilled chicken carcasses. Food Control75: 70–77.
GieraltowskiLHigaJPeraltaV, et al. (2016) National outbreak of multidrug resistant Salmonella Heidelberg infections linked to a single poultry company. PLoS ONE11(9): 1–13.
10.
GuentzelJLLiang LamKCallanMA, et al. (2008) Reduction of bacteria on spinach, lettuce, and surfaces in food service areas using neutral electrolyzed oxidizing water. Foods (Basel, Switzerland)25(1): 36–41.
11.
Hernández-PimentelVMRegalado-GonzálezCNava-MoralesGM, et al. (2020) Effect of neutral electrolyzed water as antimicrobial intervention treatment of chicken meat and on trihalomethanes formation. Journal of Applied Poultry Research29(3): 622–635.
12.
HuangYRHungYCHsuSY, et al. (2008) Application of electrolyzed water in the food industry. Food Control19(4): 329–345.
13.
International Organization for Standardization (2007) ISO 7218 – Microbiology of food and animal feeding stuffs — General requirements and guidance for microbiological examinations (Amendment 1: Food microbiology — General requirements and recommendations), 72p.
14.
KhalidNISulaimanSAb AzizN, et al. (2018) Electrolyzed water as a green cleaner: Chemical and physical characterization at different electrolysing parameters. Food Research2(6): 512–519.
15.
LiaoLBChenWMXiaoXM (2007) The generation and inactivation mechanism of oxidation-reduction potential of electrolyzed oxidizing water. Journal of Food Engineering78(4): 1326–1332.
16.
MaillardJYPascoeM (2024) Disinfectants and antiseptics: Mechanisms of action and resistance. Nature Reviews Microbiology22: 4–17.
17.
MillesAAMisraSS (1938) The estimation of the bacterial power of the blood. The Journal of Hygiene38: 732–749.
18.
Ministério da Agricultura e Pecuária (MAPA) (1998) do Brasil. Instrução Normativa no 210 (10 November 1998) – Regulamento técnico da inspeção tecnológica e higiênico-sanitária de carne de aves.Diário Oficial da União (5 March 1999), seção 1, p. 17.
19.
Morales-ParteraÁMCardoso-TosetFJurado-MartosF, et al. (2017) Survival of selected foodborne pathogens on dry cured pork loins. International Journal of Food Microbiology258: 68–72.
20.
PilottoFRodriguesLBSantosLR, et al. (2007) Antibacterial efficacy of commercial disinfectants on dirt floor used in poultry breeder houses. Brazilian Journal of Poultry Science9: 127–131.
21.
RahmanSKhanIOhDH (2016) Electrolyzed water as a novel sanitizer in the food industry: Current trends and future perspectives. Comprehensive Reviews in Food Science and Food Safety15(3): 471–490.
22.
RahmanSMParkJSongKB, et al. (2012b) Effects of slightly acidic low concentration electrolyzed water on microbiological, physicochemical, and sensory quality of fresh chicken breast meat. Journal of Food Science77(1): M35–M41.
23.
RahmanSMEParkJHWangJ, et al. (2012a) Stability of low concentration electrolyzed water and its sanitization potential against foodborne pathogens. Journal of Food Engineering113(4): 548–553.
24.
RasschaertGPiessensVScheldemanP, et al. (2013) Efficacy of electrolyzed oxidizing water and lactic acid on the reduction of Campylobacter on naturally contaminated broiler carcasses during processing. Poultry Science92(4): 1077–1084.
25.
SouzaMNLehmannFKMDe CarliS, et al. (2019) Molecular detection of Salmonella serovars Enteritidis, Heidelberg and Typhimurium directly from pre-enriched poultry samples. British Poultry Science60(4): 388–394.
26.
TanakaNFujisawaTDaimonT, et al. (1999) The effect of electrolyzed strong acid aqueous solution on hemodialysis equipment. Artificial Organs23(12): 1055–1062.
27.
ThornRMLeeSWRobinsonGM, et al. (2012) Electrochemically activated solutions: Evidence for antimicrobial efficacy and applications in healthcare environments. European Journal of Clinical Microbiology & Infectious Diseases31(5): 641–653.
U.S. Food and Drugs Administration (FDA) (2012) Department of Health and Human Services (DHHS). Part 178 indirect food additives: Adjuvants, producers AIDS and sanitizers. Code of federal regulation. Title 21, v.3. Available at: https://www.ecfr.gov/current/title-21/chapter-I/subchapter-B/part-178 (accessed: 30 September 2020)
31.
Vizzier-ThaxtonYEwingMLBonnerCM (2010) Generation and detection of trihalomethanes in chicken tissue from chlorinated chill water. Journal of Applied Poultry Research19(2): 169–173.
32.
WangHDuanDZhongyuanW, et al. (2019) Primary concerns regarding the application of electrolyzed water in the meat industry. Food Control95: 50–56.
33.
WangHQiJDuanD, et al. (2018) Combination of a novel designed spray cabinet and electrolyzed water to reduce microorganisms on chicken carcasses. Food Control86: 200–206.
34.
WesselsKRipDGouwsP (2021) Salmonella in chicken meat: Consumption, outbreaks, characteristics, current control methods and the potential of bacteriophage use. Foods (Basel, Switzerland)10: 1742.
35.
WilsmannDECarvalhoDChitolinaGZ, et al. (2020) Electrochemically-activated water presents bactericidal effect against Salmonella Heidelberg isolated from poultry origin. Foodborne Pathogens and Disease17(3): 228–233.
36.
WilsmannDEFurianTQCarvalhoD, et al. (2023) Antibiofilm activity of electrochemically activated water (ECAW) in the control of Salmonella Heidelberg biofilms on industrial surfaces. Brazilian Journal of Microbiology54(3): 2035–2045.
37.
YanJXieJ (2021) Removal of Shewanella putrefaciens biofilm by acidic electrolyzed water on food contact surfaces. LWT151: 112044.
38.
ZengXTangWYeG, et al. (2010) Studies on disinfection mechanism of electrolyzed oxidizing water on E. coli and Staphylococcus aureus. Journal of Food Science75(5): M253–M260, doi:https://doi.org/10.1111/j.1750-3841.2010.01649.x
