The influence of inoculum composition on selected bioactive and nutritional parameters of grass pea tempeh obtained by mixed-culture fermentation with Rhizopus oligosporus and Aspergillus oryzae strains
Restricted accessResearch articleFirst published online April, 2012
The influence of inoculum composition on selected bioactive and nutritional parameters of grass pea tempeh obtained by mixed-culture fermentation with Rhizopus oligosporus and Aspergillus oryzae strains
Tempeh is a popular Indonesian product obtained from legume seeds by solid-state fermentation with Rhizopus sp. The aim of this research was to study the effect of simultaneous mixed-culture fermentation of grass pea seeds on selected parameters of products as compared to traditional tempeh. The inoculum contained different ratios of Rhizopus oligosporus and Aspergillus oryzae spores. The simultaneous fermentation of grass pea seeds with inoculum consisting of 1.2 × 106R. oligosporus and 0.6 × 106A. oryzae spores (per 60 g of seeds) resulted in a product of improved quality, as compared with traditionally made tempeh (obtained after inoculation with 1.2 × 106R. oligosporus spores), at the same fermentation time. This product had radical scavenging ability 70% higher than the one obtained with pure R. oligosporus culture and contained 2.23 g/kg dm of soluble phenols. The thiamin and riboflavin levels were above three (340 µg/g dm) and two (50.50 µg/g dm) folds higher than in traditionally made tempeh, respectively. The product had 65% in vitro bioavailability of proteins and 33% in vitro bioavailability of sugars. It also contained 40% less 3-N-oxalyl-L-2, 3-diaminopropionic acid (0.074 g/kg dm), as compared to traditional tempeh.
Angulo-BejaranoPIVerdugo-MontoyaNMCuevas-RodríguezEOMilán-CarrilloJMora-EscobedoRLopez-ValenzuelaJA (2008). Tempeh flour from chickpea (Cicer arietinum L.) nutritional and physicochemical properties. Food Chemistry106(1): 106–112.
2.
AOAC 920.39 (1995). Official Methods of Analysis, 16th edn. Arlington, VA: AOAC International.
3.
ArellaFLahelySBourguingonJBHasselmanC (1996). Liquid chromatographic determination of vitamins B1 and B2 in foods. A collaborative study. Food Chemistry56(1): 81–86.
4.
AstutiMMelialaADalaisFSWahlqvistML (2000). Tempe, a nutritious and healthy food from Indonesia. Asia Pacific Journal of Clinical Nutrition9(4): 322–325.
5.
BriggsCJParrenoNCampbellCG (1983). Phytochemical assessment of Lathyrus species for the neurotoxic agent, β-N-oxalyl-L-α, β-diaminopropionic acid. Planta Medica47(3): 188–190.
6.
ChavanUDMc KenzieDBShahidiF (2001). Functional properties of protein isolates from beach pea (Lathyrus maritimus L.). Food Chemistry74(2): 177–187.
7.
ChouCCRwanJH (1995). Mycelial propagation and enzyme production in koji prepared with Aspergillus oryzae on various rice extrudates and steamed rice. Journal of Bioscience and Bioengineering79(5): 509–512.
8.
Cuevas-RodríguezEOVerdugo-MontoyaNMAngulo-BejaranoPIMilán-CarrilloJMora-EscobedoRBello-PérezLA (2006). Nutritional properties of tempeh flour from quality protein maize (Zea mays L.). LWT-Food Science and Technology39(10): 1072–1079.
9.
de ReuJCten WoldeRMde GrootJNoutMJRRomboutsFMGruppenH (1995). Protein hydrolysis during soybean tempe fermentation with Rhizopus oligosporus. Journal of Agricultural and Food Chemistry43(8): 2235–2239.
10.
Franczyk M (2002). Wpływ fermentacji nasion fasoli przy użyciu Rhizopus microsporus var. oligosporus (spT3) na skład chemiczny, zawartość substancji nieodżywczych i aktywność antyoksydacyjną podłoża. Thesis, Akademia Rolnicza w Krakowie.
11.
HongKJLeeCHKimSW (2004). Aspergillus oryzae GB-107 fermentation improves nutritional quality of food soybeans and feed soybean meals. Journal of Medicinal Food7(4): 430–435.
12.
IbrahimSSHabibaRAShattaAAEmbabyHE (2002). Effect of soaking, germination, cooking and fermentation on antinutritional factors in cowpeas. Nahrung/Food46(2): 92–95.
13.
ICC-STANDARD (1990). Determination of ash in cereals and cereal products. 104/1. Vienna, Austria: International Association for Cereal Science and Technology.
14.
IshidaHHataYIchikawaEKawatoASuginamiKImayasuS (1998). Regulation of the glucoamylase-encoding gene (glaB), expressed in solid-state culture (koji) of Aspergillus oryzae. Journal of Fermentation and Bioengineering86(3): 301–307.
15.
JinBvan LeeuwenHJPatelBDoelleHWYuQ (1999). Production of fungal protein and glucoamylase by Rhizopus oligosporus from starch processing wastewater. Process Biochemistry34(1): 59–65.
16.
KakadeFLRackisJJMc GheeJEPuskiG (1974). Determination of trypsin inhibitor activity of soy products: a collaborative analysis of an improved procedure. Cereal Chemistry51(3): 376–382.
17.
KuoYHBauHMQuemenerBKhanJKLambeinF (1995). Solid state fermentation of Lathyrus sativus seeds using Aspergillus oryzae and Rhizopus oligosporus sp. T-3 to eliminate the neurotoxin β-ODAP without loss of nutritional value. Journal of the Science of Food and Agriculture69(1): 81–89.
18.
KuoYHBauHMRozanPChowdhuryBLambeinF (2000). Reduction efficiency of the neurotoxin β-ODAP in low-toxin varieties of Lathyrus sativus seeds by solid state fermentation with Aspergillus oryzae and Rhizopus microsporus var chinensis. Journal of the Science of Food and Agriculture80(15): 2209–2215.
19.
LiemITHSteinkrausKHCronkTC (1977). Production of vitamin B-12 in tempeh, a fermented soybean food. Applied and Environmental Microbiology34(6): 773–776.
20.
LinCHWeiYTYuRCChouCC (2006). Cultivation temperature and length affect the antioxidant activity and total phenolic content of soybean koji prepared with Aspergillus awamori. Journal of Food and Drug Analysis14(1): 75–79.
21.
McCuePShettyK (2003). Role of carbohydrate-cleaving enzymes in phenolic antioxidant mobilization from whole soybean fermented with Rhizopus oligosporus. Food Biotechnology17: 27–37.
22.
MarczenkoZBalcerzakM (1998). Spektrofotometryczne Metody w Analizie Nieorganicznej, 1st edn. Warszawa: Wydawnictwo Naukowe PWN. 104–105.
23.
MatsuoM (1997). In vivo antioxidant activity of okara koji, a fermented okara, by Aspergillus oryzae. Bioscience, Biotechnology and Biochemistry61(12): 1968–1972.
24.
MillerGL (1959). Use of dinitrosalicylic acid reagent for determination of reducing sugar. Analytical Chemistry31(3): 426–428.
25.
MiszkiewiczHRozwandowiczABieleckiS (2004). Physiological and enzymatic activities of Rhizopus oligosporus in swing solid-state reactor. Chemical Papers58(6): 424–428.
26.
PekkarinenSSStockmannHSchwartzKHeinonenMHopiaAI (1999). Antioxidant activity and partitioning of phenolic acids in bulk and emulsified methyl linoleate. Journal of Agricultural and Food Chemistry47(8): 3036–3043.
27.
RandhirRWattemDShettyK (2004). Solid-state bioconversion of fava bean by Rhizopus oligosporus for enrichment of phenolic antioxidants and L-DOPA. Innovative Food Science and Emerging Technologies5(2): 235–244.
28.
RehmsHBarzW (1995). Degradation of stachyose, raffinose, melibiose and sucrose by different tempe-producing Rhizopus fungi. Applied Microbiology and Biotechnology44(1–2): 47–52.
29.
SheihICWuHYLaiYJLinCF (2000). Preparation of high free radical scavenging tempeh by newly isolated Rhizopus sp. R-69 from Indonesia. Food Science and Agricultural Chemistry2(1): 35–40.
30.
Starzyńska-JaniszewskaAStodolakBJamrózJ (2008). Antioxidant properties of extracts from fermented and cooked seeds of polish cultivars of Lathyrus sativus. Food Chemistry109(2): 285–292.
31.
StodolakBStarzyńska-JaniszewskaA (2008). The influence of tempeh fermentation and conventional cooking on anti-nutrient level and protein bioavailability (in vitro test) of grass pea seeds. Journal of the Science of Food and Agriculture88(13): 2265–2270.
32.
SwainTHillisWE (1959). Phenolic constituents of Prunus domestica L. Quantitative analysis of phenolic constituents. Journal of the Science of Food and Agriculture10: 63–68.
33.
TyczkowskaK (1977). Metoda oznaczania kwasu taninowego w ziarnach sorga. Biuletyn Informacyjny Przemysłu Paszowego4: 40–45.
34.
WieselIRehmHJBispingB (1997). Improvement of tempe fermentations by application of mixed cultures consisting of Rhizopus sp. and bacterial strains. Applied Microbiology and Biotechnology47(3): 218–225.
35.
YamaneYIFujitaJShimizuRIHiyoshiAFukudaHKizakiY (2002). Production of cellulose- and xylan-degrading enzymes by a koji mold, Aspergillus oryzae, and their contribution to the maceration of rice endosperm cell wall. Journal of Bioscience and Bioengineering93(1): 9–14.
36.
YanZYSpencerPSLiZXLiangYMWangYFWangCY (2006). Lathyrus sativus (grass pea) and its neurotoxin ODAP. Phytochemistry67(2): 107–121.
37.
YenGCChangYCSuSW (2003). Antioxidant activity and active compounds of rice koji fermented with Aspergillus candidus. Food Chemistry83(1): 49–54.
38.
YigzawYGortonLSolomonTAkaluG (2004). Fermentation of seeds of teff (Eragrostis teff), grass-pea (Lathyrus sativus) and their mixtures: aspects of nutrition and food safety. Journal of Agricultural and Food Chemistry52(5): 1163–1169.
39.
ŻyłaKWikieraAKorelewskiJŚwiątkiewiczSPironenDRLedouxDR (2000). Comparison of the efficiencies of a novel Aspergillus niger mycelium with separate and combined effectiveness of phytase, acid phosphatase and pectinase in dephosphorylation of wheat-based feeds fed to growing broilers. Poultry Science79(10): 1434–1443.
