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Abstract

Ascorbic acid (AA) and dehydroascorbic acid (DHAA) were determined by high performance liquid chromatography (HPLC) in fresh green olives as well as a diversity of commercial presentations of table olives (based on both Spanish-style green olives and directly brined olives). Fresh green olives (Manzanilla cv.) immediately after harvest contained about 9mg total ascorbic acid/100g f.w., with DHAA representing more than 90% of this amount. During the post-harvest period (till 2 weeks) the total vitamin C remained stable when olives were stored at 6°C, but significant degradation occurred at ambient temperature (~35% loss after 7 days). In commercial presentations of table olives, in general, the main contribution to the total vitamin C level appeared to come from AA added as an antioxidant, the maximum level being found in Manzanilla olives stuffed with anchovy streams (36.1mg/100g f.w.). However, in some samples (e.g. plain olives) that did declare any added AA, very low levels (0.1-0.6mg total AA/100g f.w.) were found. Our hypothesis is that, in those samples, AA would be degraded by lactic acid bacteria and/or yeast from olive fermentation, whereas pasteurisation in other presentations (e.g. stuffed olives) would stabilise added AA.

Keywords

ascorbic acid dehydroascorbic acid table olives vitamin C

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References

Casado F.J. , Sánchez A.H. , López A. , de Castro A. , Rejano L. and Montaño A. (2003). Determinación rápida de los ácidos ascórbico y dehidroascórbico en vegetales aderezados mediante HPLC . In: Proceedings of the 2nd National Congress about Food Science and Technology. Alicante, Spain: Limencop S.L. pp. 147-150 .

Favell D.J. (1998). A comparison of the vitamin C content of fresh and frozen vegetables . Food Chemistry! 62: 59-64 .

Fernández-Díez M.J., Castro R., Garrido A., Cancho F.G., Pellissó F.G., Vega M.N., Moreno A.H., Mosquera I.M., Rejano L., Quintana M.C.D., Roldán F.S., García P. and Castro A. (1985). Biotecnología de la aceituna de m esa. Madrid, Spain: CSIC.

Gökmen V. Kahranan N. , Demir N. and Acar J. (2000). Enzymatically validated liquid chromatographic method for the determination of ascorbic and dehydroascorbic acids in fruit and vegetables . Journal of Chromatography 881: 309-316 .

Howard L.A. , Wong A.D. , Perry A.K. and Klein B.P. (1999). -carotene and ascorbic acid retention in fresh and processed vegetables . Journal of Food Science 64: 929-936 .

IOOC (International Olive Oil Council) (1980). Unified Qualitative Standard Applying to Table Olives in International TradeMadrid, Spain: IOOC.

Ladrón R.V. , Castro R. and Vega M.N. (1979). Composición y valor nutritivo de algunas variedades españolas de aceitunas de mesa. III. Aceitunas verdes aderezadas envasadas . Grasas y Aceites 30: 221-226 .

Lee S.K. and Kader A.A. (2000). Pre-harvest and post-harvest factors influencing vitamin C content of horticultural crops . Postharvest Biology and Technology 20: 207-220 .

Russell L.F. (2000). Quantitative determination of water-soluble vitamins. In: Nollet, L.M.L. (ed), Food Analysis by HPLC. New York: Marcel Dekker Inc. pp. 403-476.

10.

Sánchez-Mata M.C. , Cámara-Hurtado M. , Díez-Márquez C. and Torija-Isasa M.E. (2000). Comparison of high-performance liquid chromatography and spectrofluorimetry for vitamin C analysis of green beans (Phaseolus vulgaris L.) . European Food Research and Technology 210: 220-225 .

11.

Smoot J.M. and Nagy S. (1980). Effects of storage temperature and duration on total vitamin C content of canned single-strength grapefruit juice . Journal of Agricultural and Food Chemistry 28: 417-421 .

12.

StatSoft (2000). Statistica for Windows (Computer Program Manual). Tulsa, OK, USA .

13.

USDA (Agricultural Research Service) (2003). USDA Nutrient database for standard reference, release 16. Nutrient data laboratory home page. http://www.nal.usda.gov/fnic/foodcomp.

14.

Verma K.K. , Jain A. , Sahasrabuddhey B. , Gupta K. and Mishra S. (1996). Solid-phase extraction cleanup for determining ascorbic acid and dehydroascorbic acid by titration with 2,6-dichlorophenolindophenol . Journal of AOAC International 79: 1236-1243 .

Note: Quantification of Ascorbic Acid and Dehydroascorbic Acid in Fresh Olives and in Commercial Presentations of Table Olives

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