Stability of Human Recombinant Epoetin Alfa in Commonly used Neonatal Intravenous Solutions

Abstract

OBJECTIVE:

TO measure epoetin alfa concentrations after adding it to a variety of commonly used neonatal intravenous fluids to determine the stability of epoetin alfa over time.

DESIGN:

Epoetin alfa was added to the following fluids: sterile water; NaCl 0.9%; dextrose 10% in water; dextrose 10% with albumin at concentrations of 0.01 %, 0.05%, and 0.1 %; and total parenteral nutrition solution containing either 0.5% or 2.25% amino acids. The fluid was administered through intravenous tubing, a T-connector, and catheter, and samples were collected at 0,2,4, 8, and 24 hours.

MAIN OUTCOME MEASURES:

Epoetin alfa concentrations were compared with the measured original preinfusion concentration and recorded as the percentage recovered.

RESULTS:

Concentrations declined significantly in all fluids containing less than 0.05% protein, but remained stable over 24 hours in fluids containing 0.05% or more protein.

CONCLUSIONS:

We conclude that epoetin alfa should be mixed in intravenous fluids containing at least 0.05% protein.