Kidney tissues from 20 cattle infected with Leptospira interrogans serovars hardjo, pomona, or grippotyphosa were cultured on the day of slaughter and 3, 6, and 8 days later to examine the effect of storage time on the recovery of leptospires by conventional culture methods. Leptospires were isolated from 85% of infected bovine kidney tissues cultured on day 1, and from 95%, 90%, and 90% of kidney tissues stored in transport medium at 4 C for 3, 6, and 8 days, respectively, prior to inoculation of culture media.
References
1.
AmatredjoACampbellRSFTruemanKF: 1976, A study of nephritis in beef cattle in North Queensland. Aust Vet J52: 398–402.
2.
ColeJRSulzerCRPursellAR: 1973, Improved microtechnique for the leptospiral microscopic agglutination test. Appl Microbiol25:976–980.
3.
EllinghausenHC: 1973, Growth temperatures, virulence, survival, and nutrition of leptospires. J Med Microbiol6:487–497.
4.
EllinghausenHCMcCulloughWG: 1965, Nutrition of Leptospira pomona and growth of 13 other serotypes: Fractionation of oleic albumin complex and a medium of bovine albumin and polysorbate 80. Am J Vet Res26:45–51.
5.
EllisWAMontgomeryJCassellsJA: 1985, Dihydrostreptomycin treatment of bovine carriers of Leptospira interrogans serovar hardjo. Res Vet Sci39:292–295.
6.
EllisWAO'BrienJJCassellsJ: 1981, Role of cattle in the maintenance of Leptospira interrogans serotype hardjo infection in Northern Ireland. Vet Rec108:555–557.
7.
EllisWAO'BrienJJNeillSD: 1982, Bovine leptospirosis: Microbiological and serological findings in aborted fetuses. Vet Rec110:147–150.
8.
FairbrotherJM: 1985, Effects of products of autolysis of tissue and urine on the viability, morphology, and antigenicity of Leptospira interrogans serovar pomona. J Clin Microbiol21:189–194.
9.
HoHFBlackmoreDK: 1979, Effect of chilling and freezing on survival of Leptospira interrogans serovar pomona in naturally infected pig kidneys. NZ Vet J27:121–123.
10.
JohnsonRCHarrisVG: 1967, Differentiation of pathogenic and saprophytic leptospires. I. Growth at low temperatures. J Bacteriol94:27–31.
11.
KirkbrideCA: 1984, Leptospiral abortion. In: Laboratory diagnosis of abortion in food animals, ed. KirkbrideCA, pp. 54–76. American Association of Veterinary Laboratory Diagnosticians, Madison, WI.
12.
KmetyEGaltonMMSulzerCR: 1970, Further standardization of the agglutinin-absorption test in the serology of leptospires. Bull WHO42:733–738.
13.
MichnaSW: 1959, The survival of Leptospira canicola in the renal tissue of a pig. Vet Rec71:549–552.
14.
MichnaSWEllisWDikkenH: 1974, The isolation of Leptospira hardjo from an aborting cow. Res Vet Sci17:133–135.
15.
StoennerHG: 1975, Summary report on the 1975 survey for leptospirosis in the United States. Proc US Anim Health Assoc79:145–149.
16.
StringfellowDABrownRRHansonLE: 1983, Can antibody responses in cattle vaccinated with a multivalent bacterin interfere with serologic diagnosis of disease?J Am Vet Med Assoc182:165–167.
17.
ThiermannAB: 1984, Leptospirosis: Current developments and trends. J Am Vet Med Assoc184:722–725.
18.
ThiermannABHandsakerAL: 1985, Experimental infection of calves with Leptospira interrogans serovar hardjo: Conjunctival versus intravenous route of exposure. Am J Vet Res46: 329–331.
19.
ThiermannABMcClellanRDHillHT: 1984, Improved techniques for the isolation of leptospires from swine abortion cases. Proc Annu Meet Am Assoc Vet Lab Diagn27:233–244.
