Free accessRapid communicationFirst published online 2002-5
In Situ Detection of Cellular and Abnormal Isoforms of Prion Protein in Brains of Cattle with Bovine Spongiform Encephalopathy and Sheep with Scrapie by Use of a Histoblot Technique
To detect prion protein, brains from 5 cattle naturally affected with bovine spongiform encephalopathy (BSE) and 3 sheep naturally affected with scrapie were examined and compared with brains of normal cattle and sheep using a histoblot technique. The technique enabled the in situ distinctive detection of the cellular (PrPC) and abnormal (PrPSc) isoforms of the prion protein. In BSE- or scrapie-affected brains, the PrPC signal decreased, especially in those areas where the PrPSc signal was detected.
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