Effects of various antigen-retrieval pretreatment techniques for immunohistochemical detection of Akabane virus antigen were examined with formalin-fixed, paraffin-embedded tissues taken from mice intracerebrally inoculated with the virus. The results indicated that no pretreatment is necessary for the detection of the antigen, and excessive enzymatic pretreatment of sections may lead to false-negative results.
References
1.
CharlesJA: 1994, Akabane virus. Vet Clin North Am Food Anim Pract10:525–546.
2.
HazirogluRHaritaniMNaritaM: 1987, Demonstration of Akabane virus antigen in experimentally infected mice using immunoperoxidase method. Jpn J Vet Sci49:133–135.
3.
KanaiKNunoyaTShibuyaK: 1998, Variations in effectiveness of antigen retrieval pretreatments for diagnostic immunohistochemistry. Res Vet Sci64:57–61.
4.
KonnoSMoriwakiMNakagawaM: 1982, Akabane disease in cattle: congenital abnormalities caused by viral infection: spontaneous disease. Vet Pathol19:246–266.
5.
MasonJTO'LearyTJ: 1991, Effects of formaldehyde fixation on protein secondary structure: a calorimetric and infrared spectroscopic investigation. J Histochem Cytochem39:225–229.
6.
NaritaMKawashimaK: 1993, Detection of Akabane viral antigen and immunoglobulin-containing cells in bovine fetuses by use of immunoperoxidase staining. Am J Vet Res54:420–424.
7.
ShiSRKeyMEKalraKL: 1991, Antigen retrieval in formalin-fixed, paraffin-embedded tissues: an enhancement method for immunohistochemical staining based on microwave oven heating of tissue sections. J Histochem Cytochem39:741–748.
8.
ShinRWIwakiTKitamotoTTateishiJ: 1991, Hydrated autoclave pretreatment enhances TAU immunoreactivity in formalin-fixed normal and Alzheimer's disease brain tissues. Lab Invest64: 693–702.
9.
TaylorCR: 1986, Infectious disease. In: Immunomicroscopy: a diagnostic tool for the surgical pathology, pp. 327–333. WB Saun-ders Co.Philadelphia, PA.
