Sage Journals: Discover world-class research

Abstract

This study is aimed to investigate the effect of the simultaneous incorporation of cellulose nanocrystals (CNC) and silver nanoparticles (SN) on the mechanical, biodegradability, and water vapor permeability of polylactic acid (PLA)-based films. PLA films and their nanocomposites containing different levels of CNC (0.333, 1 and 1.667 phr) and SN (0.333 phr) were prepared by solution casting method. CNC was reacted with acetic anhydride to improve its compatibility and miscibility with PLA. Fourier transform infrared (FTIR) spectroscopy, thermogravimetric analysis (TGA), tensile test, and water vapor permeability and antibacterial tests were employed to characterize the samples. The biodegradability was assessed by measuring the weight loss upon burial in the soil. FTIR spectroscopy confirmed the modification of cellulose nanocrystals. TGA test showed that partial acetylation slightly improved the thermal stability of CNC. The presence of cellulose nanocrystals increased the tensile strength and modulus of elasticity of the nanocomposite relative to pure polylactic acid. The biodegradability and water vapor permeability of the samples decreased upon CNC incorporation. The antibacterial properties of the films showed the higher resistance of the gram-positive bacteria as their cell walls include a peptidoglycan layer.

Keywords

Bionanocomposites cellulose nanocrystals silver nanoparticles polylactic acid biodegradability

Introduction

Material science research is focused on producing efficient, renewable, and environmentally friendly materials to cope with petroleum scarcity while considering environmental concerns. Recently, the “green” bio-based nanocomposites are emerging in the market as they are biodegradable, low cost, abundant, biocompatible, nontoxic, and renewable.¹ Recently, several research groups started the preparation and characterization of various types of biodegradable polymer nanocomposites suitable for a wide range of applications. Most of the studied biodegradable polymers are starch and its derivatives, polylactic acid (PLA), poly (butylene succinate), polyhydroxybutyrate, and aliphatic polyesters such as Polycaprolactone.²

Poly(lactic acid) (PLA) is a biodegradable and compostable aliphatic polyester, produced from renewable resources such as cassava, corn, starch, and sugarcane.³ Its constituent monomers are lactic acid and 2-hydroxy propionic acid. It can be produced by ring-opening and condensation polymerization.⁴ However, low elongation at break, slow degradation rate, and poor thermal stability have restricted its applications.^5,6 To overcome these problems, methods such as mixing with other polymers, crystallization optimization, use of softeners, lubricants, and fillers have been reported. One of the approaches to improve the properties of this biopolymer involves the use of nano-scale fillers and the production of nanocomposite.^7,8,9

Nanocomposites are a new class of composite materials combining nanotechnology and composite technology. The application of nanotechnology to these polymers may open new possibilities for improving not only the properties but also the cost-price efficiency. Owing to the nano-scale size of dispersed particles, these nanocomposites can exhibit markedly improved mechanical, thermal, barrier, and physicochemical properties.^10,11,12 Nanoparticles such as nanoclay, nanocellulose, silver, titanium oxide, and carbon nanotubes can be used to reinforce the nanocomposites.

The size of silver nanoparticles ranges between 1 nm and 100 nm. Numerous shapes of nanoparticles can be constructed depending on their intended application. Commonly used silver nanoparticles are spherical, but diamond, octagonal, and thin sheets can be also applied. Their extremely large surface area permits the coordination of a vast number of ligands. Silver nanoparticles applicable to human treatments are under investigation in laboratory and animal studies to assess their potential efficacy, biosafety, and biodistribution.¹³

In general, nanocellulose can be prepared in two main forms: nanofibers (CNFs) and nanocrystals (CNCs). Their main difference lies in their extraction method and appearance. Cellulose nanocrystals (CNCs) are the crystalline regions of CNFs and are known as cellulose monocrystalline regions. Due to the high crystallinity of CNCs, their elastic modulus is equivalent to that of crystalline cellulose (up to 140 GPa) which can be attributed to their hard nature and ability to form hydrogen bonds. Depending on their source of production, the size of CNCs varies from 10 to 1000 nm in length and 4–25 nm in diameter.¹⁴

Cellulose nanocrystals can be obtained by acidic hydrolysis to remove the amorphous regions from cellulose chains. These structures have shown unique features such as availability, low cost, renewability, low density, and high surface-to-volume ratio, modulus of elasticity, tensile strength, stiffness, flexibility as well as good thermal, optical, and electrical properties. Moreover, they are considered biodegradable nanocomposites.¹⁵ The most important limitation of cellulose nanoparticles in the production of nanocomposites is their incompatibility with non-polar polymers¹⁶ which can be resolved by surface modification and to decline its polar nature.^17,18,19

Based on Reza Arjmandi et al., the biodegradability of polylactic acid composites increased by partial replacement of MMT (montmorillonite) with MCC (microcrystalline cellulose) filler as compared to neat PLA.²⁰ Juan I. Moran et al. prepared poly(lactic acid)/Cellulose-nanowhisker nanocomposites for packaging applications and showed that the mechanical properties of the nanocomposites were higher than those of the neat matrix.²¹ According to the thermogravimetric analysis (TGA) studies by Achal Bhiogade and colleagues, PLA is thermally more stable than PLA-based composites.²² C. Prapruddivongs et al. reported higher permeability and antibacterial properties in the samples containing cloisite® 30B.²³

In the present study, the compatibility of CNC with polylactic acid was improved by chemical surface modification of the nanoparticles with acetic anhydride. The water vapor permeability of CNC/nanosilver-reinforced polylactic acid nanocomposite was also investigated for the first time. Their water absorption and weight loss in deionized water, as well as the mechanical performance, were also explored. Finally, the antibacterial properties of the films were assessed against Gram-positive Staphylococcus aureus and Gram-negative Escherichia coli.

Materials and methods

Materials

Polylactic acid (2003D; Natureworks; US) was used as the polymeric base material. Cellulose nanocrystals were obtained from chemical treatment of pure cellulose fibers (2.5% dryness) as produced by Nano Novin Polymer (Iranian company) in the form of a white gel. Silver nanoparticle powder with a density of 10.5 g/cm³, purity of 99.99%, and size of 20 nm was purchased (Research and Market, US). Chloroform (Merck, Germany) was used as a solvent. Acetic acid and pyridine (Merck, Germany) were employed for the chemical modification of the CNC, Acetic anhydride was also supplied from the Sigma Aldrich Company.

Preparation of the films

Polylactic acid films and their nanocomposites were prepared by the solution casting ... method; Table 2 shows the components of samples. To prepare the pure PLA film, the polylactic acid granules were dried in an oven at 60°C for 12 h before use to remove the moisture. The dried PLA was dissolved in chloroform under magnetic stirring followed by 10 min of sonication to ensure complete and uniform dissolution. Finally, the solution was poured into a glass mold and placed at room temperature for 24 h to evaporate the solvent. To prepare PLA-based nanocomposites, specified amounts of nanoparticles were added to chloroform and stirred for 8 h. To achieve better dispersion, the dissolved mixtures were sonicated for 30 min. The PLA solution and nanoparticles were then mixed at predetermined ratios and treated with a magnetic stirrer for 2 h followed by 20 min of ultrasonication. Finally, the solutions were poured into glass molds. After the solvent evaporation at room temperature, the prepared films were separated from the mold and placed in the oven at 40°C to completely remove any residual solvent, which can act as a softener. Note that prior to the tests, the films were placed in a desiccator containing phosphorus pentoxide to remove moisture and achieve a constant weight. (Table 1)

Table 1.

Components of the samples.

Samples	PLA (100%)	sCNC (phr)	SNP (phr)
PLA	100	—	—
PLA-1	100	0.333	0.333
PLA-3	100	1	0.333
PLA-5	100	1.667	0.333

CNC: cellulose nanocrystals; PLA: polylactic acid.

Chemical modification of cellulose nanocrystalline surface

Chemical modification of nanocellulose surface can increase its compatibility. Acetylation was performed by constant mixing under a nitrogen atmosphere in a balloon equipped with a condenser. To remove water, 40 g CNC suspension with 2.5% dryness was mixed with 80 mL acetic acid before acetylation. After 10 min of gentle mixing, the nanocrystals were separated from acetic acid through centrifugation. The remaining nanocrystals were transferred to a balloon to which 30 mL acetic acid and 40 mL acetic anhydride were added in one step followed by the addition of a pyridine catalyst at 5wt% relative to nanocrystals.

Figure 1 schematically shows the reaction. The resulting suspension was reacted at 80°C for 5 h under a magnetic stirrer and nitrogen atmosphere. Then, the acrylic nanocrystals were washed in an acidic medium using a centrifuge; followed by washing with the acetone-methanol mixture (1:2) four times. The acetylated CNCs were dried in an oven at 60°C for 8 h.²⁴

Figure 1.

Chemical modification of nanocellulose with acetic anhydride.²⁵

Fourier transform infrared spectroscopy

Infrared spectroscopy was used to examine the chemical structure of cellulosic nanocrystals before and after chemical modification. The fourier transform infrared (FTIR) spectra of the films were recorded in the range of 2500-500 cm⁻¹ using an FTIR spectrometer (RXI FTIR).

Tensile properties

Tensile test was carried out under ASTM D882 standard using a stretching device model STM 250 (Santam, made in Iran). The dumbbell-shaped films (0.5 × 10 cm²) were placed between the two jaws of the device at an initial distance of 50 mm and a loading speed of 2 mm/min. Five replications were considered for each sample.

Thermal gravimetric analysis

Thermal stability and thermal degradation of the samples were evaluated by TGA TMA1000 (Sanaf, Iran) using 30 mg film samples at the temperature range of 50–500°C and heating rate of 10°C/min under nitrogen atmosphere.

Water vapor permeability

Water vapor permeability was measured according to ASTM E96-95 standard. Accordingly, special vials were employed with a diameter of 2 cm and a height of 4.5 cm. The lid of these vials had a hole with a diameter of 8 mm, in which a piece of film was placed. After placing the cut pieces of the desired films and the initial weighing, the vials were transferred to a desiccator containing water placed in an incubator at 25 ± 1°C. The weight of the vials was measured every few hours for 4 days. The amount of water vapor transferred from the films is determined by measuring the weight of the vials. The water vapor transfer rate (WVTR) can be obtained by plotting the weight gain of the vials versus time. Using equation (1), water vapor permeability (WVP) was calculated

W V P = \frac{W V T R}{P (R_{1} - R_{2})} X

(1)

In the above equation:

WVTR: water vapor transfer rate (g/s.m²)

WVP: water vapor permeability (g/Pa.s.m)

X: film thickness (m)

P: pure water vapor pressure at 25°C (Pa)

R₁: relative humidity in the desiccator (100%)

R₂: relative humidity within the vial (0%).

This test was performed in triplicates.

Destruction of films in deionized water

The degradation of films in deionized water was evaluated as described by Hossain et al.²⁶ through a weight difference method. Typically, 25 mm × 25 mm films were cut and their initial weight was recorded. The samples were then immersed in glass bottles containing 25 mL of deionized water at 25 and 40°C for 10 days. The samples were then dried in an oven at 50°C until reaching a constant weight. The weight loss (%) was calculated by equation (2).

Weight loss percentage = \frac{M_{0} - M_{d s}}{M_{0}} \times 100

(2)

In the above equation:

M₀: sample weight before immersion

M_ds: weight of the dried sample after immersion

Biodegradability

The biodegradability of the samples was assessed by measuring their weight at 50°C in the beginning and after drying in the oven. The samples were then buried in the soil at a depth of 5 cm (inside plastic boxes and in the laboratory). During the test, the soil inside the boxes was kept moist by adding distilled water. To measure the extent of degradation, the samples were removed from the soil at 10-day intervals and their surface soil was washed with distilled water followed by oven-drying for 12 h. Finally, the weight of the samples was recorded. Weight loss percentage was calculated using equation (3).

Weight Loss = \frac{W_{0} - W_{d}}{W_{0}} \times 100

(3)

In the above equation:

W₀: Initial dry weight

W_d: dry weight after burial in soil

Antibacterial properties

The antibacterial properties of the films were evaluated by the disk diffusion method according to Clinical & Laboratory Standards Institute¹ standards. For this purpose, plates containing Mueller-Hinton agar medium were prepared. Wells with a diameter of 6 mm were made on the medium. Then, 100 μL bacterial suspensions with turbidity equivalent to 0.5 McFarland were uniformly cultured on the medium. The dried extract was dissolved in dimethyl sulfoxide to a concentration of 30 mg/mL extract 10 μL/well (300 μg) was poured into the wells and the plates were incubated at 37°C for 24 h. The antimicrobial activity was determined for each microorganism by measuring the growth inhibition zone.

Results and discussion

Fourier transform infrared spectroscopy of cellulose nanocrystal

Infrared spectroscopy was used to investigate the chemical structure of cellulose nanocrystals after chemical modification. Figure 2 shows the FTIR spectrum of cellulose nanocrystals before and after chemical modification. The FTIR analysis of modified cellulose nanocrystals showed an absorption peak at 1706 cm⁻¹ due to the stretching vibration of the C=O bond. But this peak is transferred toward lower wavenumbers (1780-1750) due to the effect of the methyl group attached to alpha carbon (reaction with acetic acid). This effect reduced the dual bond property of C=O and thus declined the absorption number. The stretching vibration of the C-O bond of the acetyl group at 1050 cm⁻¹ and the stretching vibration of the methyl (C=O)-CH₃ groups confirmed the formation of acetylated groups in the modified cellulose nanocrystals.²⁷

Figure 2.

Fourier transform infrared spectroscopy of pristine and modified cellulose nanocrystals.

Thermogravimetric analysis of cellulose nanocrystals

The effect of acetylation on the thermal stability of cellulose nanocrystals was evaluated by TGA, as shown in Figure 3. During thermal decomposition, nanocellulose showed a gradual and multi-stage weight loss which can be divided into three temperature zones. The first zone started at 50°C and continued up to 150°C, which can be attributed to the evaporation of water and other solvents. The second temperature range lied at 150–350°C while the third range was at 350–500°C. In the second temperature zone, approximately 50% weight loss occurred in the CNC due to the removal of the side groups formed in the preparation of cellulose nanocrystals such as sulfate. In the third stage, the rest of the compounds were decomposed.²⁸ According to Figure 3, the onset of thermal degradation for pristine and modified cellulose nanocrystals was at 210 and 245°C, respectively, close to the report of Fortunati et al. (220 and 260°C for pristine and modified cellulose nanocrystals, respectively).²⁹ The thermal stability of CNCs depends on their degree of crystallinity, that is, it decreases with declining the degree of crystallinity. As can be seen, the partial acetylation slightly improved the thermal stability of the cellulose nanocrystals. The reason could be assigned to the effect of acetylation on the crystalline structure of nanocrystalline cellulose (removal of some amorphous areas, hence, increasing the crystallinity degree). Tingaut et al. used acetylated cellulose microfibrils to improve the properties of PLA films. Their results indicated a decrement in the thermal stability of nanocellulose with increasing acetylation, due to severe degradation of crystalline areas of cellulose.³⁰

Figure 3.

Thermal analysis of modified and pristine cellulose nanocrystals.

Mechanical properties of polylactic acid-based nanocomposites

The results of the mechanical test on polylactic acid films containing different ratios of CNC and Ag NPs are presented in Figure 4. As seen, the incorporation of CNC into the PLA matrix enhanced the tensile strength and modulus. In general, the mechanical performance of composites, especially nanocomposites, depends on several factors such as compatibility of the polymer matrix with the filler, stress transfer to the filler, volume fraction of the filler, filler aspect ratio, filler orientation, and matrix crystallization.³¹ Here, the improvement of mechanical properties of nanocomposites by adding CNC to the PLA matrix can be attributed to the high crystallinity and good mechanical properties of these nanoparticles.³² As can be seen in Figure 4, the increasing trend of strength and tensile modulus by adding 1 phr of CNC was higher than other samples. The reason could be due to the formation of a rigid three-dimensional network of CNCs within the substrate and the reduced mobility of polymer chains. At higher nanoparticle contents, this increase was not observed in properties, probably due to improper dispersion and particle aggregation.³³

Figure 4.

Mechanical properties of polylactic acid and its bionanocomposites.

Water vapor permeability of polylactic acid-based films

The use of nano-scale structures results in a more uniform distribution of materials and increases the crystallinity of the film, thus, hindering the passage of water vapor. In the presence of the filler, the permeability was affected by the zigzag path which is itself changed by factors such as the aqueous activity, the filler shape, surface ratio, distribution, amount, orientation, adhesion to the substrate, crystallization, purity, porosity, and pore size.^34,35 The results of water vapor permeability of PLA-based films are presented in Figure 5.

Figure 5.

Water vapor permeability of polylactic acid and its bionanocomposites.

As can be seen, by increasing the CNC level, water vapor permeability decreased due to the high crystallinity of cellulose nanocrystals, which acted as a nucleating agent in the polymer matrix. The presence of cellulose nanoparticles in the polymer matrix prolonged the path of water molecules. Increased crystallinity of the polymer due to the presence of cellulose nanoparticles enhanced the cohesion and density between the polymer chains and reduced the inter-chain free spaces, which is another reason for the declined permeability of nanocomposite films. It was observed that the sample containing 1.667 phr CNC did not exhibit reduced permeability compared to the sample containing 1 phr, which is probably due to clumping and improper distribution at this CNC percentage.³⁶

Degradation of polylactic acid films in deionized water

Weight loss of films at 25 and 40°C

Table 2 shows the weight loss of PLA films containing cellulose and silver nanoparticles. Comparing the weight loss of the films at 25 and 40°C, the weight loss at 40°C was more intense compared to 25°C. The reason could be due to the hydrolysis of the polymer at higher temperatures, which is consistent with the results of Hossain et al.²⁶ At high temperatures, cellulose nanocrystals diffused to the surface from the polymer matrix, moreover, the CNC-polymer interface also decomposed, further increasing the weight loss.³⁷

Table 2.

Weight loss percentage of nanocomposites at 25° and 40°C.

Samples	Weight loss percentage at 25°C	Weight loss percentage at 40°C
PLA	1.4 ± 0.02	2.11 ± 0.01
PLA-1	2.79 ± 0.05	3.39 ± 0.03
PLA-3	3.47 ± 0.05	4.27 ± 0.08
PLA-5	4.16 ± 0.07	6.25 ± 0.05

PLA: polylactic acid.

Water absorption of the films at 25 and 40°C

For high moisture-sensitive packaging materials, exposure to high-humidity environments not only alters the functional properties of the packaging material but also reduces the durability of the packaging product due to moisture absorption.

Table 3 shows the trend of water absorption variation of PLA-based films containing cellulose and silver nanoparticles. The water absorption percentage of nanocomposites at 25° and 40°C showed that the temperature elevation incremented the water absorption due to the PLA hydrolysis and the separation of CNCs from the films, breaking the CNC-polymer bond, hence the formation of microcavities.³⁸

Table 3.

Water absorption of nanocomposites at 25° and 40°C.

Samples	Water absorption (%) at 25°C	Water absorption (%) at 40°C
PLA	2.07 ± 0.01	2.51 ± 0.05
PLA-1	3.59 ± 0.03	2.48 ± 0.01
PLA-3	5.03 ± 0.025	5 ± 0.03
PLA-5	5.79 ± 0.01	9.62 ± 0.06

PLA: polylactic acid.

Biodegradability of nanocomposites

The weight loss of PLA films and composites is shown in Figure 6. As can be seen, the weight loss of the samples decreased with increasing the amount of cellulose nanocrystals. The weight loss rate of the samples over time followed a linear trend and increased by prolonging the burial time in the soil. The growth of microorganisms requires adequate physical and chemical conditions such as moisture, temperature, and oxygen. Thus, the process of composite degradation depended on providing the necessary conditions for the growth and attack of microorganisms.^39,40 The decrease in the degradability of the samples due to the presence of cellulose nanocrystals can be attributed to the strong tendency of free hydroxyl groups to form complexes with polar mineral compounds of the soil, which made them impermeable to microorganisms. The occurrence of strong reactions with minerals at the soil surface indeed limited the accessibility of functional groups and reduced the biodegradability of samples.^29,41,42

Figure 6.

Weight loss of polylactic acid film and nanocomposites after burial in soil.

Cellulose nanocrystals formed long paths in the polymer substrate, prolonging the diffusion of water and oxygen molecules, thereby delaying the growth of microorganisms. Therefore, the degradability of composites decreased by elevating the CNC content.

Antibacterial properties of nanocomposites

The antibacterial properties of composite films containing Ag NPs and CNCs are presented in Table 4 against Staphylococcus aureus and Escherichia coli. These results include the growth inhibition zone diameter for the mentioned bacteria. The difference in the antibacterial activity of Ag NPs against Staphylococcus can be assigned to the differences in its cell wall structure compared to gram-negative bacteria. The difference between these two is generally related to the structure of their cell wall and their cell lining. The cell lining of gram-positive bacteria is relatively simple and encompasses 2–3 layers of cells; whereas the gram-negative bacterial cell wall has a multilayered and highly complex structure with peptidoglycan chains lacking strong cross-linking. As a result, the cell wall of gram-positive bacteria has a higher resistance due to the presence of the peptidoglycan layer, which is consistent with the results of Li et al.⁴³

Table 4.

Inhibitory zone diameter for Staphylococcus aureus and Escherichia coli.

Bacteria	Staphylococcus aureus	Escherichia coli
Samples	Diameter (mm)	Diameter (mm)
PLA	—	—
PLA-1	1 ± 0.1	2 ± 0.2
PLA-3	1 ± 0.2	2 ± 0.25
PLA-5	1 ± 0.15	2 ± 0.2

PLA: polylactic acid.

Conclusion

Polylactic acid was used in the current research to prepare biodegradable films. To improve the functional properties of the films, CNC and Ag NPs were employed. Various tests were performed to measure the film properties. The results can be summarized as

1. Acrylication of CNC was confirmed by FTIR analysis. Dispersion and TGA results also showed that the use of acetic anhydride was effective in acetylation treatment.

2. The incorporation of cellulose and silver nanoparticles into the polylactic acid film improved its mechanical properties compared to the pure PLA film. By adding more than 1 phr nano cellulose, the mechanical properties decreased.

3. Cellulose and silver nanoparticles increased the moisture absorption inhibition of films and also reduced the water vapor permeability of films. So that the film containing 1 phr CNC and 0.333 phr Ag NPs exhibited the highest reduction compared to other films.

4. According to the biodegradability test results, the presence of nanoparticles in the PLA polymer substrate leads to the formation of complexes between hydroxyl groups and polar mineral compounds in the soil, thus reducing the biodegradability of the nanocomposites.

5. The antibacterial properties of the films showed an antibacterial resistance in the samples containing Ag NPs in comparison with the Ag-free samples which were more resistant to gram-negative bacteria than the gram-positive ones.

Footnotes

Declaration of conflicting interests

The author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.

Funding

The author(s) received no financial support for the research, authorship, and/or publication of this article.

ORCID iD

Mohsen Najafi

Note

References

Takkalkar

Ganapathi

Dekiwadia

, et al. Preparation of square-shaped starch nanocrystals/polylactic acid based bio-nanocomposites: morphological, structural, thermal and rheological properties. Waste Biomass Valor 2018; 10: 3197–3211.

Sorrentino

Gorrasi

Vittoria

. Potential perspectives of bio-nanocomposites for food packaging applications. Trends Food Sci Technol 2007; 18(2): 84–95.

Satti

Shah

Marsh

, et al. Biodegradation of poly (lactic acid) in soil microcosms at ambient temperature: evaluation of natural attenuation, bio-augmentation and bio-stimulation. J Polym Envirom 2018; 26: 3848–3857.

Raquez

Habibi

Murariu

, et al. Polylactide (PLA)-based nanocomposites. Prog Polym Sci 2013; 38(10–11): 1504–1542.

Shayan

Azizi

Ghasemi

, et al. Influence of modified starch and nanoclay particles on crystallization and thermal degradation properties of cross-linked poly(lactic acid). J Polym Res 2019; 26(238): 1–12.

Akrami

Ghasemi

Azizi

, et al. A new approach in compatibilization of the poly(lactic acid)/thermoplastic starch (PLA/TPS) blends. Carbohyd Polym 2016; 144: 254–262.

Noushirvani

Ghanbarzadeh

Entezami

. Comparison of tensile, permeability and color properties of starch-based bionanocomposites containing two types of fillers: sodium montmorilonite and cellulose nanocrystal. JIPST 2011; 24: 391–402.

Dadashi

Mousavi

Emam-Djomeh

, et al. Films based on poly (lactic acid) biopolymer: effect of clay and cellulosic nanoparticles on their physical, mechanical and structural properties. JIPST 2012; 25(2): 127–136.

Manafi

Ghasemi

Karrabi

, et al. Crystallization and morphology of nanocomposites based on poly(lactic acid)/graphene nanoplatelets: effect of nanoparticles functionalization. JIPST 2015; 27(5): 383–394.

10.

De Azeredo

. Nanocomposites for food packaging applications. Food Res Int 2009; 42(9): 1240–1253.

11.

Dallas

Sharma

Zboril

. Silver polymeric nanocomposites as advanced antimicrobial agents: classification, synthetic paths, applications, and perspectives. Adv Colloid Interfac 2011; 166(1–2): 119–135.

12.

Sorrentino

Gorrasi

Vittoria

. Potential perspectives of bio-nanocomposites for food packaging applications. Trends Food Sci Tech 2007; 18(2): 84–95.‏

13.

Graf

Vossen

Imhof

, et al. General method to coat colloidal particles with silica. Langmuir 2003; 19(17): 6693–6700.

14.

Jonoobi

Rahamin

Rafieian

. Cellulose nanocrystal properties and their applications. IJWP 2015; 6(1): 167–192.

15.

Bhat

Dasan

Khan

, et al. Application of nanocrystalline cellulose: processing and biomedical applications. Cellulose-Reinforced Nanofibre Composites 2017; (pp. 215–240). Elsevier Inc. doi: 10.1016/B978-0-08-100957-4.00009-7

16.

Cunha

Gandini

. Turning polysaccharides into hydrophobic materials: a critical review. Part 1. Cellulose. Cellulose 2010; 17(5): 875–889.

17.

Matsumura

Sugiyama

Glasser

. Cellulosic nanocomposites. I. Thermally deformable cellulose hexanoates from heterogeneous reaction. J Appl Polym Sci 2000; 78(13): 2242–2253.

18.

Gousse

Chanzy

Cerrada

, et al. Surface silylation of cellulose microfibrils: preparation and rheological properties. Polymer 2004; 45(5): 1569–1575.

19.

Ramos

Morgado

El Seoud

, et al. Acetylation of cellulose in LiCl-N, N-dimethylacetamide: first report on the correlation between the reaction efficiency and the aggregation number of dissolved cellulose. Cellulose 2011; 18(2): 385–392.

20.

Arjmandi

Hassan

Mohamad Haafiz

, et al. Biodegradability and thermal properties of hybrid montmorillonite/microcrystalline cellulose filled polylactic acid composites: effect of filler ratio. Polym Polym Compos 2016; 24(9): 741–746.

21.

Moran

Ludueña

Phuong

, et al. Processing routes for the preparation of poly(lactic acid)/cellulose- nanowhisker nanocomposites for packaging applications. Polym Polym Compos 2016; 24(5): 341–346.

22.

Bhiogade

Kannan

. Studies on thermal and degradation kinetics of cellulose micro/nanoparticle filled polylactic acid (PLA) based nanocomposites. Polym Polym Compos 2021; 29: 1–14.

23.

Prapruddivongs

Sombatsompop

Jayaraman

, et al. Effect of organoclay incorporation on mechanical, barrier and thermal properties and anti-bacterial performance of PLA and PLA composites with triclosan and wood flour. Polym Polym Compos 2014; 22(7): 643–652.

24.

Lin

Huang

Chang

, et al. Surface acetylation of cellulose nanocrystal and its reinforcing function in poly (lactic acid). Carbohyd Polym 2011; 83(4): 1834–1842.

25.

Chen

, et al. Polylactide/acetylated nanocrystalline cellulose composites preparedby a continuous route: a phase interface-property relation study. Carbohyd Polym 2016; 146: 58–66.

26.

Hossain

KMZ

Ahmed

Parsons

, et al. Physico-chemical and mechanical properties of nanocomposites prepared using cellulose nanowhiskers and poly (lactic acid). J Mater Sci 2012; 47(6): 2675–2686.

27.

Paula

ELD

Mano

Duek

EAR

, et al. Hydrolytic degradation behavior of PLLA nanocomposites reinforced with modified cellulose nanocrystals, Quim Nova 2015; 38(8): 1014–1020.

28.

Roman

Winter

. Effect of sulfate groups from sulfuric acid hydrolysis on the thermal degradation behavior of bacterial cellulose. Biomacromolecules 2004; 5(5): 1671–1677.

29.

Fortunati

Armentano

Zhou

, et al. Multifunctional bionanocomposite films of poly (lactic acid), cellulose nanocrystals and silver nanoparticles. Carbohyd Polym 2012; 87(2): 1596–1605.

30.

Tingaut

Zimmermann

Lopez-Suevos

. Synthesis and characterization of bionanocomposites with tunable properties from poly (lactic acid) and acetylated microfibrillated cellulose. Biomacromolecules 2009; 11(2): 454–464.

31.

Jonoobi

Mathew

Abdi

, et al. A comparison of modified and unmodified cellulose nanofiber reinforced polylactic acid (PLA) prepared by twin screw extrusion. J Polym Environ 2012; 20(4): 991–997.

32.

Kord

Jari

Najafi

, et al. Effect of nanoclay on the decay resistance and physicomechanical properties of natural fiber-reinforced plastic composites against white-rot fungi (Trametes versicolor). J Thermoplast Compos 2014; 27(8): 1085–1096.

33.

Roohani

Habibi

Belgacem

, et al. Cellulose whiskers reinforced polyvinyl alcohol copolymers nanocomposites. Eur Polym J 2008; 44(8): 2489–2498.

34.

Sanchez-Garcia

Gimenez

Lagaron

. Development and characterization of novel nanobiocomposites of bacterial poly(3- hydroxybutirate), layered silicates and poly(e-caprolactone). J Appl Polym Sci 2008; 108: 2787–2801.

35.

Sanchez-Garcia

Gimenez

Lagaron

, Morphology and barrier properties of solvent cast composites of thermoplastic biopolymers and purified cellulose fibers. Carbohyd Polym 2008; 71: 235–244.

36.

Paralikar

Simonsen

Lombardi

. Poly (vinyl alcohol)/cellulose nanocrystal barrier membranes. J Membr Sci 2008; 320(1–2): 248–258.

37.

Shen

Yang

Ying

, et al. Preparation and mechanical properties of carbon fiber reinforced hydroxyapatite/polylactide biocomposites. J Mater Sci Mater M 2009; 20(11): 2259–2265.

38.

Yew

Yusof

Ishak

, et al. Water absorption and enzymatic degradation of poly (lactic acid)/rice starch composites. Polym Degrad Stabil 2005; 90(3): 488–500.

39.

Ibach

Clemons

Schumann

. Wood-plastic composites with reduced moisture: effects of chemical modification on durability in the laboratory and field. Ninth International Conference on Wood & Biofiber Plastic Composites, 21–23 May 2007, Monona Terrace Community & Convention Center, Madison, WI, USA: Forest Products Society, 259–266.

40.

Kord

Jari

Najafi

41.

Roohani

Kord

Motie

, et al. Biodegradation behaviors of cellulose nanocrystals -PVA nanocomposites. IJWP 2014; 5(2): 1–14.

42.

Rhim

Hong

. Tensile, water vapor barrier and antimicrobial properties of PLA/nanoclay composite films. LWT Food Sci Technol 2009; 42(2): 612–617.

43.

Jia

, et al. Cellulose–silver nanocomposites: microwave-assisted synthesis, characterization, their thermal stability, and antimicrobial property. Carbohyd Polym 2011; 86(2): 441–447.

Physico-mechanical properties of polylactic acid bio-nanocomposites filled by hybrid nanoparticles

Abstract

Keywords

Introduction

Materials and methods

Materials

Preparation of the films

Chemical modification of cellulose nanocrystalline surface

Fourier transform infrared spectroscopy

Tensile properties

Thermal gravimetric analysis

Water vapor permeability

Destruction of films in deionized water

Biodegradability

Antibacterial properties

Results and discussion

Fourier transform infrared spectroscopy of cellulose nanocrystal

Thermogravimetric analysis of cellulose nanocrystals

Mechanical properties of polylactic acid-based nanocomposites

Water vapor permeability of polylactic acid-based films

Degradation of polylactic acid films in deionized water

Weight loss of films at 25 and 40°C

Water absorption of the films at 25 and 40°C

Biodegradability of nanocomposites

Antibacterial properties of nanocomposites

Conclusion

Footnotes

Declaration of conflicting interests

Funding

ORCID iD

Note

References