Cell proliferation in CPC scaffold with a central channel

Abstract

Calcium phosphate cement (CPC) scaffold design should improve nutrient and cell transfer to the scaffold centre. To achieve this goal, a channel network with proper channel diameters should be incorporated into the scaffold. In this study, CPC scaffolds with a single central channel were fabricated indirectly using a stereolithography rapid prototyping (RP) technology. The diameters of the central channels ranged from 402 μm to 1988 μm. These scaffolds were seeded with rabbit marrow stem cells (MSCs) labeling DiI and cultured for 5 days. Cell observation on the wall of the central channels was performed. The data of the experimental point revealed that cell coverage was from approximately 18% (1988 μm) to approximately 35% (592 μm). There was a significant increase from day 1 to day 5 in cell coverage in the same channel. The cell area coverage increased lineally with the central channel diameter when the channel diameter was less than approximately 789 μm. Afterwards (from 789 to 1988 μm), the relationship between cell area coverage and channel diameter was also linear relationship. But the increase was more slowly than before. Preliminary demonstration from the data that the minimum channel diameter required for cell migration into and adhesion on CPC scaffold was approximately 72 μm. These results are promising for the development of optimal scaffold with a three-dimensional channel network.