This work focuses on designing bilayered constructs by combining electrospun poly-DL-Lactide (PDLLA) fibers and Bioglass®-derived scaffolds for development of osteochondral tissue replacement materials. Electrospinning was carried out using a solution of 5 wt/v% PDLLA in dimethyl carbonate. The PDLLA layer thickness increased from 2 to 150 µm with varying electrospinning time. Bioactivity studies in simulated body fluid showed that HA mineralization decreased as the thickness of the PDLLA layer increased. A preliminary in vitro study using chondrocyte cells (ATDC5) showed that cells attached, proliferated and migrated into the fibrous network, confirming the potential applicability of the bilayered scaffolds in osteochondral defect regeneration.
O'SheaTMMiaoX. Bilayered scaffolds for osteochondral tissue engineering. Tissue Eng Part B Rev2008; 14: 447–464.
7.
ShapiroFKoideSGlimcherMJ. Cell origin and differentiation in the repair of full-thickness defects of articular-cartilage. J Bone Joint Surg Amer Vol1993; 75A: 532–553.
8.
BuckwalterJA. Articular cartilage: injuries and potential for healing. J ortho Sports Phys Ther1998; 28: 192–202.
9.
DounchisJSCouttsRDAmielD. Cartilage repair with autogenic perichondrium cell/polylactic acid grafts: a two-year study in rabbits. J Ortho Res2000; 18: 512–515.
10.
ShortkroffSBaroneLHsuHPWrennCChiTMinasT. Healing of chondral and osteochondral defects in a canine model: the role of cultured chondrocytes in regeneration of articular cartilage. Biomaterials1996; 17: 147–154.
11.
GlowackiJ. In vitro engineering of cartilage. J Rehab Res Dev2000; 37: 171–177.
12.
GrandeDEBreitbartASMasonJPaulinoCLaserJSchwartzRE. Cartilage tissue engineering: current limitations and solutions. Clin Ortho Rel Res1999; S176–S185.
13.
TemenoffJSMikosAG. Review: tissue engineering for regeneration of articular cartilage. Biomaterials2000; 21: 431–440.
14.
WoodfieldTBFBezemerJMPieperJSVanBlitterswijkCARiesleJ. Scaffolds for tissue engineering of cartilage. Crit Rev Eukar Gene Exp2002; 12: 209–236.
15.
SchaeferDMartinIShastriPPaderaRFLangerRFreedLE. In vitro generation of osteochondral composites. Biomaterials2000; 21: 2599–2606.
16.
DormerNHBerklandCJDetamoreMS. Emerging techniques in stratified designs and continuous gradients for tissue engineering of interfaces. Ann Biomed Eng2010; 38: 2121–2141.
17.
MoffatKWangIRodeoSLuH. Orthopedic interface tissue engineering for the biological fixation of soft tissue grafts. Clin Sports Med2009; 28: 157–176.
18.
WangXGroganSPRieserFWinkelmannVMaquetVBergeML. Tissue engineering of biphasic cartilage constructs using various biodegradable scaffolds: an in vitro study. Biomaterials2004; 25: 3681–3688.
19.
GuoXMWangCYDuanDMDescampsMZhaoQDongLZ. Repair of osteochondral defects with autologous chondrocytes seeded onto bioceramic scaffold in sheep. Tissue Eng2004; 10: 1830–1840.
20.
KandelRAGrynpasMPiliarRLeeJWangJWaldmanS. Repair of osteochondral defects with biphasic cartilage-calcium polyphosphate constructs in a sheep model. Biomaterials2006; 27: 4120–4131.
21.
AllanKSPiliarRMWangJGrynpasMDKandelRA. Formation of biphasic constructs containing cartilage with a calcified zone interface. Tissue Eng2007; 13: 167–177.
22.
SchaeferDMartinIJundtGSeidelHHebererMGrodzinskyA. Tissue-engineered composites for the repair of large osteochondral defects. Arthritis Rheumat2002; 46: 2524–2534.
23.
ShaoXXGohJCHHutmacherDWLeeEHGeZG. Repair of large articular osteochondral defects using hybrid scaffolds and bone marrow-derived mesenchymal stem cells in a rabbit model. Tissue Eng2006; 12: 1539–1551.
24.
GhoshSVianaJCReissRLManoJF. Bi-layered constructs based on poly(L-lactic acid) and starch for tissue engineering of osteochondral defects. Mater Sci Eng C, Biomim Mater Sens Syst2008; 28: 80–86.
25.
ChenGSatoTTanakaJTateishiT. Preparation of a biphasic scaffold for osteochondral tissue engineering. Mater Sci Eng C, Biomim Mater Sens Syst2006; 26: 118–123.
26.
LiaoCJLinYJJChiangHChiangSFWangYHJiangCC. Injecting partially digested cartilage fragments into a biphasic scaffold to generate osteochondral composites in a nude mice model. J Biomed Mater Res Part A2007; 81A: 567–577.
27.
YunosDMAhmadZBoccacciniAR. Fabrication and characterisation of electrospun poly-D,L-lactide (PDLLA) fibrous coating on 45S5 Bioglass® substrate for bone tissue engineering applications. J Chem Technol Biotechnol2010; 85: 768–774.
28.
ChenQZThompsonIDBoccacciniAR. 45S5 Bioglass®-derived glass–ceramic scaffolds for bone tissue engineering. Biomaterials2006; 27: 2414–2425.
29.
ChenQZBoccacciniAR. Poly(D,L-lactic acid) Coated 45S5 Bioglass®-based scaffolds: processing and characterisation. J Biomed Mater Res2006; 77A: 445–457.
30.
TakadamaHKokuboT. How useful is SBF in predicting in vivo bone bioactivity?Biomaterials2006; 27: 2907–2915.
31.
TareRSHowardDPoundJCRoachHIOreffoROC. Tissue engineering strategies for cartilage generation – micromass and three dimensional cultures using human chondrocytes and a continuous cell line. Biochem Biophys Res Commun2005; 333: 609–621.
32.
ShieldsKJBeckmanMJBowlinGLWayneJS. Mechanical properties and cellular proliferation of electrospun collagen type II. Tissue Eng2004; 10: 1510–1517.
33.
KwonOHLeeISKoY-GMengWJungK-HKangI-K. Electrospinning of microbial polyester for cell culture. Biomed Mater2007; 2: S52–S58.
34.
ChenFLeeCNTeohSH. Nanofibrous modification on ultra-thin poly(epsilon-caprolactone) membrane via electrospinning. Mater Sci Eng C2007; 27: 325–332.
35.
DeitzelJMKleinmeyerJHarisDTanNCB. The effect of processing variables on the morphology of electrospun nanofibres and textiles. Polymer2001; 42: 261–272.
36.
WangYWangGChenLLiHYinTWangB. Electrospun nanofibre meshes with tailored architectures and patterns as potential tissue-engineering scaffolds. Biofabrication2009; 1: 015001 (9pp)–015001 (9pp).
37.
GibsonPSchreuder-GibsonH. Patterned electrospray fibre structures. Int Nonwovens J2004; 13: 34–41.
38.
ZhangDChangJ. Electrospinning of three-dimensional nanofibrous tubes with controllable architectures. Nano Lett2008; 8: 3283–3287.
39.
MalafayaPBReisRL. Bilayered chitosan-based scaffolds for osteochondral tissue engineering: influence of hydroxyapatite on in vitro cytotoxicity and dynamic bioactivity studies in a specific double-chamber bioreactor. Acta Biomater2009; 5: 644–660.
40.
CurtisAWilkinsonC. Topographical control of cells. Biomaterials1997; 18: 1573–1583.
41.
WooKMChenVJMaPX. Nano-fibrous scaffolding architecture selectively enhances protein adsorption contributing to cell attachment. J Biomed Mater Res2003; 67A: 531–537.
42.
GradSZhouLGogolewskiSAliniM. Chondrocytes seeded onto poly (L/DL-lactide) 80%/20% porous scaffolds: a biochemical evaluation. J Biomed Mater Res2003; 66A: 571–579.
43.
AllemannFMizunoSEidKYatesKEZaleskeDGlowackiJ. Effects of hyaluronan on engineered articular cartilage extracellular matrix gene expression in 3-dimensional collagen scaffolds. J Biomed Mater Res2001; 55: 13–19.
