Organic-inorganic composites, prepared from bone-bonding bioactive ceramics and organic polymers, are useful for novel bone substitutes having mechanical properties analogous to natural bone. We synthesized composites from cellulose and carbonate hydroxyapatite (CHAp) in situ through mechanochemical reaction. They contained B-type CHAp analogous to bone apatite. They showed a bending strengths of 10-13 MPa and Young’s modulus of 1.5-2.2 GPa. We predicted their microstructure by comparing the measured density with the theoretical one. Cellulose was assumed to be distributed in the pore of CHAp at low cellulose content, and in grain boundaries of CHAp at high cellulose content. The composites formed calcium phosphate on their surfaces in simulated body fluid, meaning that they have a potential to be bioactive.
Hench, L. L. , Splinter, R. J. , Allen, W. C. and Greenlee, T. K. (1972). Bonding Mechanism at the Interface of Ceramics Prosthetic Materials , J. Biomed. Mater. Res. Symp., 2: 117-141 .
2.
Jarcho, M. , Kay, J. F. , Gumaer, K. I. , Doremus, R. H. and Drobeck, H. P. (1977). Tissue, Cellular and Subcellular Events at a Bone-ceramic Hydroxyapatite Interface , J. Bioeng., 1(2): 79-92 .
3.
Kokubo, T. , Shigematsu, M. , Nagashima, Y. , Tashiro, M. , Nakamura, T. and Yamamuro, T. (1982). Apatite- and Wollastonite-containing Glass-ceramics for Prosthetic Application , Bull. Inst. Chem. Res. Kyoto Univ., 60(3-4): 260-268 .
4.
Kikuchi, M. , Itoh, S. , Ichinose, S. , Shinomiya, K. and Tanaka, J. (2001). Self-organization Mechanism in a Bone-like Hydroxyapatite/Collagen Nanocomposite Synthesized in vitro and its Biological Reaction in vivo , Biomaterials, 22(2): 1705-1711 .
5.
Chang, M. C. , Ko, C. C. and Douglas, W. H. (2003). Preparation of Hydroxyapatite-gelatin Nanocomposite , Biomaterials, 24(17): 2853-2862 .
6.
Yamaguchi, I. , Tokuchi, K. , Fukuzaki, H. , Koyama, Y. , Takakuda, K. , Monma, H. and Tanaka, J. (2001). Preparation and Microstructure Analysis of Chitosan/hydroxyapatite Nanocomposites , J. Biomed. Mater. Res., 55(1): 20-27 .
7.
Okazaki, M. , Ohmae, H. and Hino, T. (1989). Insolubilization of Apatite-collagen Composites by UV Irradiation , Biomaterials, 10(8): 564-568 .
8.
Okazaki, M. , Ohmae, H. , Takahashi, J. , Kimura, H. and Sakuda, M. (1990). Insolubilized Properties of UV-irradiated CO3 apatite-collagen Composites , Biomaterials, 11(8): 568-572 .
9.
Linhart, W. , Peters, F. , Lehmann, W. , Schwarz, K. , Schilling, A. F. , Amling, M. , Rueger, J. M. and Epple, M. (2001). Biologically and Chemically Optimized Composites of Carbonated Apatite and Polyglycolide as Bone Substitution Materials , J. Biomed. Mater. Res., 54(2): 162-171 .
10.
Niiranen, H. , Pyhalto, T. , Rokkanen, P. , Kellomaki, M. and Tormala, P. (2004). In vitro and in vivo Behavior of Self-reinforced Bioabsorbable Polymer and Self-reinforced Bioabsorbable Polymer/bioactive Glass Composites , J. Biomed. Mater. Res., 69A(4): 699-708 .
11.
Gibson, I. and Bonfield, W. (2002). Novel Synthesis and Characterization of an AB-type Carbonate-substituted Hydroxyapatite , J. Biomed. Mater. Res., 59(4): 697-708 .
12.
Hasegawa, M. , Doi, Y. and Uchida, A. (2003). Cell-mediated Bioresorption of Sintered Carbonate Apatite in Rabbits , J. Bone Joint Surg. Br., 85-B(1): 142-147 .
13.
Landi, E. , Celotti, G. , Logroscino, G. and Tampieri, A. (2003). Carbonated Hydroxyapatite as Bone Substitute , J. Eur. Ceram. Soc., 23(15): 2931-2937 .
14.
Landi, E. , Tampieri, A. , Celotti, G. , Vichi, L. and Sandri, M. (2004). Influence of Synthesis and Sintering Parameters on the Characteristics of Carbonate Apatite , Biomaterials, 25(10): 1763-1770 .
15.
Tadic, D. and Epple, M. (2003). Mechanically Stable Implants of Synthetic Bone Mineral by Cold Isostatic Pressing , Biomaterials, 24(25): 4565-4571 .
16.
Nordstrom, E. G. and Karlsson, K. H. (1990). Carbonate-doped Hydroxyapatite , J. Mater. Sci.: Mater. Med., 1(3): 182-184 .
17.
Suchanek, W. L. , Shuk, P. , Byrappa, K. , Riman, R. E. , TenHuisen, K. S. and Janas, V. F. (2002). Mechanochemical-hydrothermal Synthesis of Carbonated Apatite Powders at Room Temperature , Biomaterials, 23(3): 699-710 .
18.
Zhang, L. , Ruan, D. and Gao, S. (2002). Dissolution and Regeneration of Cellulose in NaOH/Thiourea Aqueous Solution , J. Polym. Sci. Part B: Polym. Phys., 40(14): 1521-1529 .
19.
Ruan, D. , Zhang, L. , Mao, Y. , Zeng, M. and Li, X. (2004). Microporous Membranes Prepared from Cellulose in NaOH/thiourea Aqueous Solution , J. Membr. Sci., 241(2): 265-274 .
20.
Zheng, H. , Zhou, J. , Du, Y. and Zhang, L. (2002). Cellulose/Chitin Films Blended in NaOH/Urea Aqueous Solution , J. Appl. Polym. Sci., 86(7): 1679-1683 .
21.
Sveensson, A. , Nicklasson, E. , Harrah, T. , Panilaitis, B. , Kaplan, D. L. , Brittberg, M. and Gatenholm, P. (2005). Bacterial Cellulose as a Potential Scaffold for Tissue Engineering of Cartilage , Biomaterials, 26(4): 419-431 .
22.
Miyamoto, T. , Takahashi, S. , Ito, H. and Inagaki, H. (1989). Tissue Biocompatibility of Cellulose and Its Derivatives , J. Biomed. Mater. Res., 23(1): 125-133 .
23.
Kokubo, T. , Kushitani, H. , Sakka, S. , Kitsugi, T. and Yamamuro, T. (1990). Solutions Able to Reproduce in vivo Surface-structure Changes in Bioactive Glass-ceramic A-W , J. Biomed. Mater. Res., 24(6): 721-734 .
24.
Koutsopoulos, S. (2002). Synthesis and Characterization of Hydroxyapatite Crystal: A Review Study on the Analytical Methods , J. Biomed. Mater. Res., 62(4): 600-612 .
25.
Rehman, I. and Bonfield, W. (1997). Characterization of Hydroxyapatite and Carbonated Apatite by Photo Acoustic FTIR Spectroscopy , J. Mater. Sci.: Mater. Med., 8(1): 1-4 .
26.
Nemoto, R. , Nakamura, S. , Isobe, T. and Senna, M. (2001). Direct Synthesis of Hydroxyapatite-Silk Fibroin Nano-Composite Sol via a Mechanochemical Route , J. Sol-Gel. Sci. Tech., 21(1-2): 7-12 .
27.
Yoshida, A. , Miyazaki, T. , Ishida, E. and Ashizuka, M. (2004). Preparation of Bioactive Chitosan-hydroxyapatite Nanocomposites for Bone Repair through Mechanochemical Reaction , Mater. Trans., 45(4): 994-998 .
28.
Nagakura, S. , Iguchi, Y. , Ezawa, H. , Iwamura, S. , Sato, F. and Kubo, R. (1998). Rikagaku-jiten, 5th edn (in Japanese), p. 274-274, Iwanami Shoten Publishers, Tokyo .
