Adsorption of Bovine Serum Albumin onto Surface-Modified Polyhydroxyethyl Methacrylate Beads

Abstract

The adsorption of bovine serum albumin (BSA) onto poly(2-hydroxyethyl methacrylate) (PHEMA) beads modified by using the pair of hexamethylene diisocyonate–suberic acidbis-N-hydroxy succinimide has been studied as a function of protein concentration and adsorption time. The adsorption studies were carried out in phosphate buffer solution (PBS) at pH = 7.4. The isotherm data have been analysed using the Langmuir model and the adsorption parameters Q⁰ and b were calculated. It is determined that the adsorbed amount of BSA increases by the increase of the adsorption time and BSA concentration until a certain value. PHEMA beads were characterized by using FTIR spectra and SEM analysis. The adsorption of BSA onto PHEMA beads were clearly observed from SEM micrographs. The swelling tests of the beads were performed at 37°C in PBS.

Keywords

bovine serum albumin (BSA)poly(2-hydroxyethyl methacrylate) (PHEMA) beads surface modification hexamethylene diisocyonate–suberic acidbis-N-hydroxy succinimide protein adsorption Langmuir isotherm

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