A poly(DL-lactic-co-glycolic acid) (PLGA) sandwich fibrinogen/ adipose stem cell (ADSC) construct was fabricated to generate smooth muscle tissue. The mechanical properties and ADSC compatibility of PLGA, poly(ethylene glycol-1,6-hexamethyl diisocyanate-caprolactone) i.e. polyurethane (PU), gelatin, alginate, and fibrin composites were evaluated for vascular smooth muscle tissue generation. Synthetic PLGA and PU combined with natural gelatin, alginate, and fibrin for strength and cell compatibility were found to be effective. A trilayer construct was designed and built with a microporous inner PLGA layer to provide nutrient, oxygen, and metabolite transfer while the outer PLGA layer with no pores prevented leakage during in vitro culture and in vivo implantation. The fibrin layer suitably accommodated ADSC growth, migration, proliferation, and differentiation inside the construct. This design has the potential for wide use in tissue engineering and complex organ construction.
Wang, X.H., Yan, Y.N. and Zhang, R.J. ( 2007). Rapid Prototyping as a Tool for Manufacturing Bioartificial Livers, Trends Biotechnol., 25: 505-513.
3.
Xu, W., Wang, X.H., Yan, Y.N. and Zhang, R.J. ( 2008). Rapid Prototyping of Polyurethane for the Creation of Branched Vascular Systems, J. Bioact. Compat. Polym., 23: 154-170. (Featured by Nature China on May 3, 2008).
4.
Wang, X.H., Yan, Y.N. and Zhang, R.J. ( 2009). Recent Trends and Challenges in Complex Organ Manufacturing , Tissue Eng: Part B, in press.
5.
Rouwkema, J., Rivron, N.C. and van Blitterswijk, C.A. ( 2008). Vascularization in Tissue Engineering, Trends Biotechnol., 26: 434-441.
6.
Caspi, O., Lesman, A., Basevitch, Y., Gepstein, A., Arbel, G., Habib, M. et al. (2007). Tissue Engineering of Vascularized Cardiac Muscle from Human Embryonic Stem Cells, Circ. Res., 100: 263-272.
7.
Yin, D.Z. , Wang, X.H., Yan, Y.N. and Zhang, R.J. (2007). Preliminary Studies on Peripheral Nerve Regeneration along a New Polyurethane Conduit, J. Bioact. Compat. Polym., 22: 143-159.
8.
Yan, Y.N. , Wang, X.H., Yin, D.Z. and Zhang, R.J. (2007). A New Polyurethane/Heparin Vascular Graft for Small-Caliber Vein Repair, J. Bioact. Compat. Polym., 22: 323-341.
9.
Kim, B.S. , Putnam, A.J., Kulik, T.J. and Mooney, D.J. (1998). Optimizing Seeding, and Culture Methods to Engineer Smooth Muscle Tissue on Biodegradable Polymer Matrices, Biotechol. Bioeng., 57: 46-54.
10.
Ziegler, T. and Nerem, R.M. (1994). Tissue Engineering a Blood Vessel: Regulation of Vascular Biology by Mechanical Stresses, J. Cell Biochem., 56: 204-209.
11.
Grassl, E.D. , Oegema, T.R. and Tranquillo , R.T. (2002). Fibrin as an Alternative Biopolymer to Type-I Collagen for the Fabrication of a Media Equivalent, J. Biomed. Mater. Res., 60: 607-612.
12.
Aper, T., Teebken, O.E., Steinhoff, G. and Haverich, A. ( 2004). Use of a Fibrin Preparation in the Engineering of a Vascular Graft Model, Eur. J. Vasc Endovasc. Surg., 28: 296-302.
13.
Yao, R. , Zhang, R.J., Yan, Y.N. and Wang, X.H. (2009). In vitro Angiogenesis of 3D Tissue Engineered Adipose Tissue, J. Bioact. Compat. Polym., 24: 5-29.
14.
Xu, M.E., Yan, Y.N., Liu, H.X., Yao, R. and Wang, X.H. ( 2008). Establishing a Multicellular Model by Three-Dimensional Cell-Assembly Technique for Metabolic Syndrome, Nature Precedings, Posted 8 Jan 2008.
15.
Xu, M.E. , Yan, Y.N., Liu, H.X., Yao, R. and Wang, X.H. (2009). Control Adipose-Derived Stromal Cells Differentiation into Adipose and Endothelial Cells in a 3-D Structure Established by Cell-Assembly Technique, J. Bioact. Compat. Polym., 24(S1): 31-47.
16.
Li, S.J. , Xiong, Z., Wang, X.H., Yan, Y.N., Liu, H.X. and Zhang, R.J. (2009). Direct Fabrication of a Hybrid Cell/Hydrogel Construct via a Double-nozzle Assembling Technology , J. Bioact. Compat. Polym., 24: 249-265.
17.
Li, S.J., Yan, Y.N., Xiong, Z., Weng, C.Y., Zhang, R.J. and Wang, X.H. ( 2009). Gradient Hydrogel Construct based on an Improved Cell Assembling System, J. Bioact. Compat. Polym., 24(S1): 84-99.
18.
Wang, X.H. and Sui, S.C.Optimizing the Step by Step Forming Processes for Fabricating a PLGA Sandwiched Cell/Hydrogel Construct, J. Bioact. Compat. Polym., submitted.
19.
Wang, X.H. and Sui, S.C.Characterization of a PLGA Sandwiched Cell/ Fibrin Construct and Induction of the Adipose Stem Cells (ADSCs) into Smooth Muscle Cells, J. Bioact. Compat. Polym., submitted.
20.
Wang, X.H. and Sui, S.C.Pulsatile Culture of a PLGA Sandwiched Cell/ Hydrogel Construct Fabricated by a Step by Step Mold/Extraction Method, Artif. Organs., submitted.
21.
Xu, W., Wang, X.H., Yan, Y.N. and Zhang, R.J. ( 2008). A Olyurethane-Gelatin Hybrid Construct for the Manufacturing of Implantable Bioartificial Livers, J. Bioact. Compat. Polym. , 23: 409-422.
22.
Cui, T.K. , Yan, Y.N., Zhang, R.J., Liu, L., Xu, W. and Wang, X.H. (2009). Rapid Prototyping of a Double Layer Polyurethane-Collagen Conduit for Peripheral Nerve Regeneration , Tissue Eng. Part C, 15: 1-9.
23.
Cui, T.K. , Wang, X.H., Yan, Y.N. and Zhang, R.J. (2009). Rapid Prototyping a New Polyurethane-Collagen Conduit and its Schwann Cell Compatibility, J. Bioact. Compat. Polym., 24(S1): 5-17.
24.
Wang, X.H., Cui, T.K., Yan, Y.N., Zhang, R.J. ( 2009). Peroneal Nerve Regeneration using a Unique Bilayer Polyurethane-Collagen Guide Conduit, J. Bioact. Compat. Polym., 24: 109-127.
25.
Walden, R., L’Italien, G.J., Megerman, J. and Abbott, W.M. ( 1980). Matched Elastic Properties and Successful Arterial Grafting , Arch. Surg., 115: 1166-1169.
26.
Stekelenburg, M., Rutten, M.C.M., Snoeckx, L.H.E.H. and Baaijens , F.P.T. (2009). Dynamic Straining Combined with Fibrin Gel Cell Seeding Improves Strength of Tissue-Engineered Small-Diameter Vascular Grafts, Tissue Eng. Part A, 15: 1081-1089.
27.
Kim, B.S. and Mooney, D.J. (1998). Engineering Smooth Muscle Tissue with a Predefined Structure, J. Biomed. Mater. Res., 41: 322-332.
