Preparation of Polyelectrolyte-Containing Nanofibers by Electrospinning in the Presence of a Non-Ionogenic Water-Soluble Polymer

Abstract

The first successful preparation of nanofibers of a polyampholyte (N-carboxyethylchitosan) by electrospinning was achieved by adding a non-ionogenic water-soluble polymer to the spinning solution. Using this approach, other polyelectrolytes, poly(2-acryloylamido-2-methylpropanesulphonic acid) (PAMPS), and copolymers of 2-acryloylamido-2-methylpropane-sulphonic acid (AMPS) and acrylic acid [P(AMPS-co-AA)] were also electrospun into nanofibers. The non-ionogenic water-soluble polymers were polyacrylamide (PAAm) and poly(vinyl alcohol) (PVA). The average diameters of the electrospun nanofibers were in the range 50-260nm. The average diameter of the nanofibers significantly decreased with increasing polyelectrolyte content. The electrospun nanofibers were crosslinked by heat treatment at 100, 120 or 150°C for the N-carboxyethylchitosan/PAAm pair and at 90°C in the case of P(AMPS-co-AA)/PVA. The presence of an ionizable low-molecular-weight compound (7-iodo-8-hydroxyquinoline-5-sulphonic acid, SQ) led to a more than two-fold decrease in the diameter of the nanofibers and to the appearance of defects. The SQ-containing nanofibers showed antimicrobial activity against pathogenic microorganisms.

Keywords

electrospinning water-resistant nanofibers polyelectrolytes non-ionogenic polymers heat-induced crosslinking N-carboxyethylchitosan PAMPS AMPS copolymers polyacrylamide poly(vinyl alcohol)antimicrobial activity

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References

Deitzel, J. , Kleinmeyer, J. , Harris, D. and Beck Tan, N. (2001). The Effect of Processing Variables on the Morphology of Electrospun Nanofibers and Textiles , Polymer, 42: 261-272 .

Huang, Z. , Zhang, Y. , Kotaki, M. and Ramakrishna, S. (2003). A Review on Polymer Nanofibers by Electrospinning and their Applications in Nanocomposites , Composites Sci. Techn., 63: 2223-2253 .

Deitzel, J. , Kleinmeyer, J , Hirvonen, J. and Beck Tan, N. (2001). Controlled Deposition of Electrospun poly(ethylene oxide) Fibers , Polymer, 42: 8163-8170 .

Morozov, V. , Morozova, T. and Kallenbach, N. (1998). Atomic Force Microscopy of Structures Produced by Electrospraying Polymer Solutions , Int. J. Mass Spectrom., 178: 143-159 .

Ding, B. , Kim, H. , Lee, S. , Shao, C. , Lee, D. , Park, S. , Kwag, G. and Choi, K. (2002). Preparation and Characterization of a Nanoscale Poly(vinyl alcohol) Fiber Aggregate Produced by an Electrospinning Method , J. Polym. Sci.: Part B: Polym. Phys., 40: 1261-1268 .

Theron, S. , Zussmana, E. , Yarin, A. (2004). Experimental Investigation of the Governing Parameters in the Electrospinning of Polymer Solutions , Polymer, 45: 2017-2030 .

Park, W. , Jeong, L. , Yoo, D. and Hudson, S. (2004). Effect of Chitosan on Morphology and Conformation of Silk Fibroin Nanofibers , Polymer, 45: 7151-7157 .

Nikolova, A. , Manolova, N. and Rashkov, I. (1998). Rheological Characterization of Aqueous Solutions of Mixtures of Chitosan and Polyoxyethylene , Polym. Bull., 41: 115-121 .

Spasova, M. , Manolova, N. , Paneva, D. and Rashkov, I. (2004). Preparation of Chitosan Containing Nanofibers by Electrospinning Chitosan/Poly(ethylene oxide) Mixed Solutions , e-Polymers, 056 1-12 .

10.

Duan, B. , Dong, C. , Yuan, X. and Yao, K. (2004). Electrospinning of Chitosan Solutions in Acetic Acid with Poly(ethylene oxide) , J. Biomater. Sci. Polymer Edn., 15: 797-811 .

11.

Ohkawa, K. , Cha, D. , Kim, H. , Nishida, A. and Yamamoto, H. (2004). Electrospinning of chitosan . Macromol. Rapid Commun., 25: 1600-1605 .

12.

Sashiwa, H. , Yamamori, N. , Ichinose, Y. , Sunamoto, J. and Aiba, S. (2003). Chemical Modification of Chitosan, 17a: Michael Reaction of Chitosan with Acrylic Acid in Water , Macromol. Biosci., 3: 231-233 .

13.

Stoilova, O. , Koseva, N. , Manolova, N. and Rashkov, I. (1999). Polyelectrolyte Complex Between Chitosan and Poly(2-acryloylamido-2-methylpropanesulfonic acid) , Polym. Bull., 43: 67-73 .

14.

Paneva, D. , Stoilova, O. , Manolova, N. and Rashkov, I. (2003). Novel Poly-electrolyte Complexes Between Chitosan and Poly(2-acryloylamido-2-methylpropanesulfonic acid-co-acrylic acid) , e-Polymers, 033 1-13 .

15.

Watter, M.T. and Wang D.W. (1985). Polyacrylamide, in: Encyclopedia of Polymer Science and Engineering, John Wiley and Sons Inc. , p. 202, New York, USA, Vol. 1.

16.

Fisher, L. , Sochor, A. and Tan, J. (1977). Chain Characteristics of Poly(2- acryloylamido-2-methylpropanesulfonic acid) Polymers. 1. Light-scattering and Intrinsic Viscosity Studies , Macromolecules,10: 949-954 .

17.

Fong, H. , Chun, I. and Reneker, D. (1999). Beaded Nanofibers Formed During Electrospinning , Polymer, 40: 4585-4592 .

18.

Mincheva, R., Manolova, N., Paneva, D. and Rashkov, I., to be published.

19.

Rangaraj, A. , Vangani, V. and Rakshit, A. (1997). Synthesis and Characterization of Some Water Soluble Polymers , J. Appl. Polym. Sci., 66: 45-56 .

20.

Ferreira, L. , Vidal, M. and Gil, H. (2001). Design of a Drug-Delivery System Based on Polyacrylamide Hydrogels. Evaluation of Structural Properties , Chem. Educator, 6: 100-103 .

21.

Aggour, Y. and Atia, A. (2001). Thermal Properties of Copolymers of 2- Acryloylamido-2-methylpropanesulphonic Acid With Some Vinyl Monomers , Macromol. Symp., 175: 377-386 .

22.

Kumeta, K. , Nagashima, I. , Matsui, S. and Mizoguchi, K. (2003). Crosslinking Reaction of Poly(vinyl alcohol) with Poly(acrylic acid) (PAA) by Heat Treatment: Effect of Neutralization of PAA , J. Appl. Polym. Sci., 90: 2420-2427 .