Abstract
A simple method using direct and indirect polyclonal antibody-based immunoperoxidase techniques were used to detect E. insidiosa antigen in various organ specimens (kidney, heart, skin, joint of knee and bursa of Fabricius) in experimentally infected turkeys. The specimens were fixed in formalin and ethanol and then embedded in paraffin. By using the above methods E. insidiosa bacilli were detected intracellularly in macrophages and endothelial cells of the vessels. They were also found extracellularly in the lumen of the vessels, either free or in aggregations of bacteria trapped in fibrin thrombi. It is interesting to note the presence of the E. insidiosa bacillus antigen in the cytoplasm of the endothelial cells of the vessels, which indicates intense phagocytic activity.
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