The pH of conventional peritoneal dialysis solution is normally in the range of 5.0 to 5.5, because acid has been added during the manufacturing process to prevent caramelization of dextrose during sterilization. We studied the effects of normalizing the pH of conventional peritoneal dialysis solution on superoxide production by normal human neutrophils. At a pH of 6.0, superoxide generation was 4.07±2.56 (SD) nanomoles per million cells. With normalization of pH to 7.4, superoxide production was 19.3± 7.3 (p < 0.001).
The results suggest that the unphysiologic acidity of conventional peritoneal dialysis solution has deleterious consequences on neutrophil superoxide formation.
KramerP., BroyerM., BrunnerF.P.Combined report on regular dialysis and transplantation in Europe, XIV, 1983. Proc Eur Dial Transplant Assoc1984; 21: 5–65.
2.
DuweA.K., VasS.I., WeatherheadJ.W.Effects of the composition of peritoneal dialysis fluid on chemiluminescence, phagocytosis, and bactericidal activity in vitro. Infect Immun1981; 33: 130–5.
3.
AlobaidiH.M., ColesG.A., DaviesM., LloydD.Host defense in continuous ambulatory peritoneal dialysis: the effect of the dialysate on phagocyte function. Nephrol Dial Transplant1986; 1: 16–21.
4.
AlobaidiH.M.M., DaviesM., TopleyN., ColesG.A., LloydD.Oxygen metabolism of peritoneal phagocytes. Abst 4th Int Symp Perit Dial, Venice, Italy-, June 29-July 2, 1987; Perit Dial Bull (suppl)1987; 7(2): S2.
5.
BoyümA.Isolation of lymphocytes, granulocytes, and macrophages. Scand J Immunol1976; 5(Suppl 5): 9–15.
6.
MasseyV.The microestimation of succinate and the extinction coefficient of cytochrome c. Biochim Biophys Acta1959; 34: 255–6.
7.
JohnstonR.B., KeeleB.B., MisraH.P.The role of superoxide anion generation in phagocytic bactericidal activity. Studies with normal and chronic granulomatous disease leukocytes. J Clin Invest1975; 55: 1357–72.
8.
DaugirdasJ.T., ChaudhryA.W., IngI.L.Alkalinization of peritoneal dialysis solutions with sodium hydroxide or sodium bicarbonate. Artif Organs1988; 12: 203–5.
9.
RubinJ., RogersW.A., TaylorH.M.Peritonitis during continuous ambulatory peritoneal dialysis. Ann Intern Med1980; 92: 7–13.
ZimmerliW., LewP.D., CohenH.J., WaldvogelF.A.Comparative superoxide-generating system of granulocytes from blood and peritoneal exudates. Infect Immun1984; 46: 625–30.
12.
ReedW.P., LightP.D., NewmanK.A.Biofilm on Tenckhoff catheters: a possible source for peritonitis. MaherJ.F., WinchesterJ.F., eds. Frontiers in peritoneal dialysis.New York, New York: Field, Rich and Associates, Inc., 1986; 176–80.
13.
VasS.I., DuweA., WeatherheadJ.Natural defence mechanisms of the peritoneum: the effect of peritoneal dialysis fluid on polymorphonuclear cells. In: AtkinsR.C., ThomsonN.M., FarrellPC; eds. Peritoneal dialysis.Edinburgh: Churchill Livingstone, 1981; pp 41-51.
14.
HendersonI.S., CouperI.A., LumsdenA.Potentially irritant glucose metabolites in unused CAPD fluid. In: MaherJ.F., WinchesterJ.F.Frontiers in peritoneal dialysis.New York, New York: Field, Rich and Associates, Inc., 1986; 261–4.
15.
IngT.S., DaugirdasJ.T., PurandareW.Facilitating aseptic mixing of sterile substances. Am J Hosp Pharm1985; 42: 1511.
