Suspension of living hepatocytes were microencapsulated inside 300 micron mean diameter alginate artificial cells. The galactosamine fulminant hepatic failure rat model was used. 48 hours after the injection of galactosamine, grade II coma hepatic failure rats were divided into pairs. One of the pair was randomly chosen for the control group, and the other for the treated group. Each rat in the control group received one peritoneal injection of microcapsules containing no hepatocytes. Each rat in the treated group received one peritoneal injection of microcapsules containing hepatocytes. The survival of the treated group is significantly higher than the control group.
ChangT.M.S., McIntoshF.C., MasonS.G.Semipermeable aqueous microcapsules: I. Preparation and properties.Can J Physiol Pharmacol1966; 44: 115–28.
3.
ChangT.M.S.Artificial Organs.Springfield, II., USA: Charles C Thomas Publr, 1972; 212.
4.
ChangT.M.S., PoznanskyM.J.Semipermeable microcapsules containing catalase for enzyme replacement in acatalsaemic mice.Nature1968; 218: 242–5.
5.
ChangT.M.S.The in vivo effects of semipermeable microcapsules containing L-asparaginase on 6C3HED lymphosarcoma.Nature1971; 229: 117–8.
6.
SiuChongE., ChangT.M.S.In vivo effects of intraperitoneally injected L-asparaginase solution and L-asparaginase immobilized within semipermeable nylon microcapsules with emphasis on blood L-asparaginase, ‘body’ L-asparaginase, and plasma L-asparagine levels.Enzyme1974; 18: 218–39.
7.
PoznanskyM.J., ChangT.M.S.Comparison of the enzyme kinetics and immunological properties of catalase immobilized by microencapsulation and catalase in free solution for enzyme replacement.Biochim Biophys Acta1974; 334: 103–15.
8.
ChangT.M.S.Biomedical Applications of Immobilized Enzymes and Proteins.New York, USA: Plenum Publishing Corp, I:1977; I: 415.
9.
ChiritoE., ReiterB., ListerC., ChangT.M.S.Artificial liver; the effect of ACAC microencapsulated charcoal hemoperfusion on fulminant hepatic failure.Artif Organs1977; 1(1): 76–83.
10.
ChangT.M.S., HoB.L.Hemoperfusion and Artificial Organs.Beijing, China: China Academic Publr, 464, 1985.
11.
ChangT.M.S.Artificial cells in medicine and biotechnology.Appl Biochem Biotechnol1984; 10: 5–24.
12.
LimF.Microencapsulation of living cells and tissues — 1983 review and update.Appl Biochem Biotechnol1984; 10: 81–6.
13.
SunA.M., O'SheaG.M., GoosenM.F.A.Injectable microencapsulated islet cells as a bioartificial pancreas.Appl Biochem Biotechnol1984; 10: 87–100.
14.
LambertiF.V., EvangelistaR.A., BlysniukJ., WheatleyM.A., SeftonM.V.Microencapsulation of mammalian cells in polyacrylates.Appl Biochem Biotechnol1984; 10: 101–4.
