Galactosamine-induced fulminant hepatic failure rats were used for in vivo studies of the removal of tyrosine and total free phenol by means of hemoperfusion over immobilized tyrosinase within artificial cells. The elevated plasma tyrosine level was decreased to about half the value after one hour of hemoperfusion. The studies of tyrosine clearance suggest that it is efficient and remains constant throughout the one-hour hemoperfusion. In those galactosamine-induced fulminant hepatic failure rats with increased total free phenol levels hemoperfusion over tyrosinase artificial cells significantly lowered the level.
BrennerW., EdwardsL., JeppssonB., JamesJ.H., FischerJ.E.: Amino acid alterations in rats with galactosamine induced hepatitis.Gastroenterology, 77, A6, 1979.
3.
ChangT.M.S.: Artificial Cells.Charles C. Thomas.Springfield, 1972.
4.
ChangT.M.S.: Biomedical Applications of Immobilized Enzymes and Proteins.Plenum Press, New York, 1977.
5.
ChangT.M.S., ListerC., ChiritoE., O'KeefeP., Resur-reccionE.: Effects of hemoperfusion rate and time of initiation of ACAC charcoal hemoperfusion on the survival of fulminant hepatic failure rats.Trans. Am. Soc. Artif. Intern. Organs, 24, 243, 1978.
6.
ChiritoE., ReiterB., ListerC., ChangT.M.S.: Artificial liver: the effect of ACAC microencapsulated charcoal hemoperfusion on fulminant hepatic failure.Artif. Organs, 1(1), 76, 1977.
7.
ChiritoE., ListerC., ChangT.M.S.: Biochemical, hematological and histological changes in fulminant hepatic failure rat model for artificial liver assessment.Artif. Organs, 3(1), 42, 1979.
FischerJ.E.: On the occurrence of false neurochemical transmitters. In: Artificial Liver Support, (Ed. WilliamsR., Murray-LyonI.M.), Pitman Medical, London, 31, 1975.
10.
HollowayC.J., BrunnerG., SchmidtE., SchmidtF.W.: Rapid photometric assay of total free phenols in serum for prognosis in liver failure and for monitoring effectiveness of hemoperfusion. In: Hemoperfusion, Kidney and Liver Supports and Detoxification. (Eds. SidemanS., ChangT.M.S.), Hemisphere Publishing Corp., Washington, D.C., p. 139, 1979.
11.
PhillipsR.E.: Tyrosine in serum. In: Manual of Fluorometric Clinical Procedures, G.K. Turner Associates, Palo Alto, Calif., 48, 1967.
12.
RecordC.P., BuxtonB., ChaseR.A., CurzonG., Murray-LyonI.M., WilliamsR.: Plasma and brain amino acids in fulminant hepatic failure and their relationship to hepatic encephalopathy.Eur. J. Clin. Invest., 6, 387, 1976.
13.
ShuC.D., ChangT.M.S.: Tyrosinase immobilized within artificial cells for detoxification in liver failure: I.Preparation and in vitro studies. Int. J. Artif. Organs, 3, 287, 1980.
14.
WaalkersT.P., UndenfriendS.: A fluorometric method for the estimation of tyrosine in plasma and tissues.J. Lab. and Clin. Med., 50, 733, 1957.
