Rat liver microsomes and cytosol have been co-immobilized by microencapsulation into artificial cells. The immobilized enzyme retained good enzyme activity. Enzyme kinetics, storage stability, pH profiles and temperature profiles have been analyzed.
ChangT.M.S., ed., Microencapsulation and Artificial Cells.New York: Humana Press, 1984.
5.
ChangT.M.S., YuY.T., GrunwaldJ.: Artificial cells immobilized multienzyme systems and cofactors.Enzyme Engineering6: 451–461, 1982.
6.
BrunnerG.: Advances in the development of immobilized enzymes for future extracorporeal liver support. In: BrunnerG., SchmidtF.W., eds. Artificial Liver Support.Berlin: Springer-Verlag230–235, 1981.
7.
DentiE., FrestonJ.W., MarchisioM., KolffW.J.: Towards a bioartificial drug metabolizing system: gel immobilized liver cell microsomes.Trans. Amer. Soc. Artif. Int. Organs22: 693–700, 1976.
8.
ChangT.M.S., Artificial Cells, Charles C. Thomas Publisher, Springfield, III, 1972.
9.
YuY.T., ChangT.M.S.: The effects of polymerresolvent compositions on the formation of collodion membrane artificial cells.Biomat, Med. Dev., Artif. Organs8: 273–281, 1980.
10.
ChangT.M.S.: Artificial cells with regenerating multienzyme systems.Methods in Enzymology112: 195–203, 1985.
11.
ApplebyC.A., MortonR.K.: Lactic dehydrogenase and cytochrome b2 from yeast.Purification and Crystallization. Biochem. J.71: 492, 1959.
12.
MastersB.S.S., WilliamsC.H.Jr., KaminH.: The preparation and properties of microsonal TPNH-cytochrome C reductase from pig liver.Methods in Enzymology, Vol. 10, 565–573, 1982.
