The analysis of live cells and tissue is gaining increasing importance in research and development. A number of demands are thus being made on the cultivation and analysis of cell response as well. For certain research approaches, in vitro three-dimensional (3D) cultivation best mimics natural conditions. Nevertheless, the imaging of such 3D environments is still a problem. To understand cell function in 3D, a number of in vivo imaging methods have been developed. This article introduces this field and proposes some interesting and promising imaging techniques for this approach.
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