In this paper we describe two cases of naturally occurring leishmaniasis in dogs in which Leishmania sp. amastigotes are found within the cytoplasm of fibroblasts. The infected cells were identified histologically, immunohistochemically, and ultrastructurally as fibroblasts. This is the first report of leishmaniasis in any species in which amastigotes were identified in vivo within fibroblasts.
ChangKP:
Leishmania infection of human skin fibroblasts in vitro: absence of phagolysosomal fusion after induced phagocitosis of promastigotes, and their intra cellular transformation.
Am J Trop Med Hyg27
(6):
1084–1096,
1978
2.
ChangKPFongDBrayRS:
Biology of leishmania and leishmaniasis.
In:
Leishmaniasis,
ed. ChangKPBrayRS,
pp. 2–30.
Elsevier Science Publisher,
1985
3.
MartinezA:
Leishmaniasis experimental canina.
Thesis,
pp. 23–28.
Universidad de Córdoba, Córdoba,
1991
4.
MoorePF:
Utilization of cytoplasmic lysozyme immunoreactivity as a histiocytic marker in canine histiocytic disorders.
Vet Pathol23:
757–762,
1986
5.
NeafieRCConnorDH:
Visceral leishmaniasis.
In:
Pathology of Tropical and Extraordinari Disease,
ed. BinfordCHConnorDH,
vol. 1,
pp. 265–272.
Armed Forces Institute of Pathology,
Washington, DC,
1976
RidleyMJWellsCW:
Macrophage-parasite interaction in the lesions of cutaneous leishmaniasis: an ultrastructural study.
Am J Parasitol123:
79–85,
1986
8.
VosJHVan den InghTSGAMMisdorptWRamaekersFCSVan MilFNDe NeijsM:
An immunohistochemical study of canine tissues with vimentin, desmin, glial fibrillary acidic protein, and neurofilament antisera.
J Vet Med (A)36:
561–575,
1989
9.
WickramasingheSNAbadallaSHKasiliEG:
Ultrastructure of bone marrow in patients with visceral leishmaniasis.
J Clin Pathol40:
267–275,
1987
10.
WilliamsRO:
Invasion of immune dendritic cells by Leishmania major and Leishmania mexicana mexicana.
J Parasitol74:
186–187,
1988
