Monoclonal antibody (MAb) B72.3 reacts with a tumor-associated glycoprotein, designated TAG-72. TAG-72 has been identified in many human carcinomas but generally is not found in normal human tissues. Because of its proven utility in the diagnosis of human carcinomas, MAb B72.3 was applied to several different types of canine carcinomas. Five types of formalin-fixed, paraffin-embedded canine carcinomas were evaluated for immunoreactivity with MAb B72.3 by use of an avidin biotin immunoperoxidase complex method. Samples were considered positive when ≥5% of all malignant cells contained a distinct intracellular stain. Immunoreactivity for MAb B72.3 was observed in 6/9 (67%) pulmonary adenocarcinomas, 7/13 (54%) transitional cell carcinomas, 7/11 (64%) mammary adenocarcinomas, 7/11 (64%) nasal adenocarcinomas, and 1/2 (50%) prostatic adenocarcinomas. The average cellular staining for positive carcinomas was 25%. Normal canine tissues from similar anatomic sites had little or no individual cell immunoreactivity. These preliminary results indicate that some canine carcinomas may express a tumor-associated antigen that is similar to TAG-72 and that MAb B72.3 immunoreactivity may be of diagnostic significance in classifying animal tumors.
BattistaPMuraroRMammarellaSCuriaMCColasanteARosiniSLestiGSaccoRFrenchDFratiLCostantiniRM:
Complementary reactivities of anti-car-cinoembryonic antigen and antitumor-associated glycoprotein 72 monoclonal antibodies in lung carcinomas.
Cancer Res50:
6987–6994,
1990
2.
ColcherDEstebanJMCarrasquilloJASugarbakerPReynoldsJCBryantGLarsonSMSchlomJ:
Quantitative analyses of selective radiolabeled monoclonal antibody localization in metastatic lesions of colorectal cancer patients.
Cancer Res47:
1185–1189,
1987
3.
ColcherDHoran HandPNutiMSchlomJ:
A spectrum of monoclonal antibodies reactive with human mammary tumor cells.
Proc Natl Acad Sci USA78:
3199–3203,
1981
4.
EstebanJMColcherDSugarbakerPCarrasquilloJABryantGThorAReynoldsJCLarsonSMSchlomJ:
Quantitative and qualitative aspects of radiolocalization in colon cancer patients of intravenously administered MAb B72.3.
Int J Cancer39:
50–59,
1987
5.
GoldDVMattesMJ:
Monoclonal antibody B72.3 reacts with a core region structure of O-linked carbohydrates.
Tumor Biol9:
137–144,
1988
6.
Horan HandPNutiMColcherDSchlomJ:
Definition of antigenic heterogeneity and modulation among human mammary carcinoma cell populations using monoclonal antibodies to tumor-associated antigens.
Cancer Res43:
728–735,
1983
7.
HsuSMRaineLFangerH:
Use of avidin-biotin-per-oxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures.
J Histochem Cytochem29:
577–580,
1981
8.
JohnstonVGSchlomJPatersonAJBennettJMagnaniJLColcherD:
Analysis of human tumor-associated glycoprotein (TAG-72) identified by monoclonal antibody B72.3.
Cancer Res46:
850–857,
1986
9.
JohnstonWWSzpakCALottichSCThorASchlomJ:
Use of a monoclonal antibody (B72.3) as a novel immunohistochemical adjunct for the diagnosis of carcinoma in fine needle aspiration biopsies specimens.
Hum Pathol17:
501–513,
1986
10.
JohnstonWWSzpakCAThorASchlomJ:
Phenotypic characterization of lung cancers in fine needle aspiration biopsies using monoclonal antibody B72.3.
Cancer Res46:
6462–6470,
1986
11.
KjeldsenTClausenHHirohashiSOgawaTIijimaHHakomoriS:
Preparation and characterization of monoclonal antibodies directed to the tumor-associated O-linked sialosyl-2,6 α-N-acetylgalactosaminyl (Scalosyl-Tn) epitope.
Cancer Res48:
2214–2220,
1988
12.
KlugTLSattlerMAColcherDSchlomJ:
Monoclonal antibody immunoradiometric assay for an antigenic determinant (CA 72) on a novel pancarcinoma antigen (TAG-72).
Int J Cancer38:
661–669,
1986
13.
KohlerMMilsteinC:
Continuous cultures of fused cells secreting antibody of pre-defined specificity.
Nature (Lond)256:
494–497,
1975
14.
LyubskySMadariagaJLozowskiMMiskrikiYSchussAChaoSLundyJ:
A tumor-associated antigen in carcinoma of the pancreas defined by monoclonal antibody B72.3.
Am J Clin Pathol89:
160–167,
1988
15.
MartinSEMoshiriSThorAVilasiVChuEWSchlomJ:
Identification of adenocarcinoma in cytospin preparations of effusions using monoclonal antibody B72.3.
Am J Clin Pathol86:
10–18,
1986
16.
MattesMJMajorPPGoldenbergDMDionASHutterRVPKleinKM:
Patterns of antigen distribution in human carcinomas.
Cancer Res50
(Suppl):
880–884,
1990
17.
MazurMTShultzJJ:
Prostatic adenocarcinoma: evaluation of immunoreactivity to monoclonal antibody B72.3.
Am J Clin Pathol93:
466–470,
1990
18.
MolinoloASimpsonJFThorASchlomJ:
Enhanced tumor binding using immunohistochemical analyses by second generation anti-tumor-associated glycoprotein 72 monoclonal antibodies versus monoclonal antibody B72.3 in human tissue.
Cancer Res50:
1291–1298,
1990
19.
NutiMYoshioATeramatoYAMariani-CostantiniRHoran HandPColcherDSchlomJ:
A monoclonal antibody (B72.3) defines patterns of distribution of a novel tumor-associated antigen in human mammary carcinoma cell populations.
Int J Cancer29:
539–545,
1982
20.
OhuchiNThorANoseMFujitaJKyogokuMSchlomJ:
Tumor-associated glycoprotein (TAG-72) detected in adenocarcinomas and benign lesions of the stomach.
Int J Cancer38:
643–650,
1986
21.
ParhamDMCoghillGRobertsonAJ:
Critical evaluation of monoclonal antibody staining of breast carcinoma.
J Clin Pathol42:
810–813,
1989
22.
SoomroSShoushaS:
Monoclonal antibody B72.3 in benign breast lesions.
J Clin Pathol43:
1026–1028,
1990
23.
StramignoniDBowenRAtkinsonBFSchlomJ:
Differential reactivity of monoclonal antibodies with human colon adenocarcinomas and adenomas.
Int J Cancer31:
543–552,
1983
24.
TavassoliFAJonesMWMajesteRMBratthauerGLO'LearyTJ:
Immunohistochemical staining with monoclonal antibody B72.3 in benign and malignant breast disease.
Am J Surg Pathol14:
128–133,
1990
25.
ThorAGorsteinFOhuchiNSzpakCAJohnstonWWSchlomJ:
Tumor-associated glycoprotein (TAG-72) in ovarian carcinomas defined by monoclonal antibody B72.3.
J Natl Cancer Inst76:
995–1003,
1986
26.
ThorAOhuchiNSzpakCAJohnstonWWSchlomJ:
Distribution of oncofetal antigen tumor-associated glycoprotein-72 defined by monoclonal antibody B72.3.
Cancer Res46:
3118–3124,
1986
27.
ThorAViglioneMJMuraroROhuchiNSchlomJGorsteinF:
Monoclonal antibody B72.3 reactivity with human endometrium: a study of normal and malignant tissues.
Int J Gynecol Pathol6:
235–247,
1987
28.
XuMRealFXWeltSSchusslerMHOettgenHFOldLJ:
Expression of TAG-72 in normal colon, transitional mucosa, and colon cancer.
Int J Cancer44:
985–989,
1989
