Urine sediments of dogs with experimentally induced ethylene glycol poisoning were examined by light microscopy and X-ray diffraction. Massive calcium oxalate crystalluria was observed in all poisoned dogs. By light microscopy, the frequency with which six-sided hippurate-like prisms and envelope forms of calcium oxalate dihydrate occurred was approximately equal. The hippurate-like crystals were shown to be calcium oxalate monohydrate by X-ray diffractometry.
References
1.
BoveK.E.:
Ethylene glycol toxicity.
Am J Clin Pathol45:
46–50,
1966.
2.
BradleyG.M.BensonE.S.:
Examination of the urine. In:
Tod-Sanford Clinical Diagnosis by Laboratory Methods,
ed. DavidsohnHenry,
pp. 15–83,
ed. 15.
W.B. Saunders, Philadelphia,
1974.
3.
BurnsJ.R.BirdwellF.:
Changes in calcium oxalate crystal morphology as a function of concentration.
Invest Urol18:
174–177,
1980.
4.
DanaJ.D.DanaE.S.:
The System of Mineralogy,
pp. 1099–1103,
7th ed.John Wiley and Sons, New York,
1966.
5.
EmmettM.MarinsR.G.:
Clinical use of the anion gap.
Medicine (Baltimore)56:
38–54,
1977.
KramerJ.W.BistlineD.SheridanP.EmersonC:
Identification of hippuric acid crystals in the urine of ethylene glycol-intoxicated dogs and cats.
J Am Vet Med Assoc184:
584,
1984.
13.
LepageI.TawashiR.:
Growth and characterization of calcium oxalate dihydrate crystals (Weddellite).
J Pharm Sci71:
1059–1062,
1982.
14.
McChesneyE.W.GoldbergE.W.ParekhC.K.:
Reappraisal of the toxicology of ethylene glycol. II. Metabolism studies in laboratory animals.
Food Cosmet Toxicol9:
21–38,
1971.
15.
OtnesB.MontgomeryO.:
Method and reliability of crystallographic stone analysis.
Invest Urol17:
314–319,
1980.
16.
ParryM.F.WallachR.:
Ethylene glycol poisoning.
Am J Med57:
143–150,
1974.
17.
Powder Diffraction Files No. 15–762, 17–540 and 20–231.
Joint Committee on Powder Diffraction Standards, Philadelphia,
1979.
18.
RileyJ.H.StahrH.M.O'BrienS.RileyM.G.I.:
Urine and tissue oxalate and hippurate levels in ethylene glycol intoxication in the dog.
Vet Hum Toxicol24:
331–334,
1982.
19.
RogersA.L.:
Analysis of renal calculi by X-ray diffraction and electron microprobe: A comparison of two methods.
Invest Urol19:
25–28,
1981.
20.
RushtonH.G.SpectorM.RodgersA.L.HughsonM.MaguraC.E.:
Developmental aspects of calcium oxalate tubular deposits and calculi induced in rat kidneys.
Invest Urol19:
52–57,
1981.
21.
ScullyR.E.GalbabineJ.J.McNeelyB.V.:
Case records of the Mass Gen Hosp, case 38–1979.
N Engl J Med30:
650–657,
1979.
22.
TerlinksyA.S.GrochowskiJ.GeolyK.L.StanchB.S.HefterL.:
Identification of atypical calcium oxalate crystalluria following ethylene glycol ingestion.
Am J Clin Pathol76:
223–226,
1981.
23.
ThrallM.A.GrauerG.F.MeroK.N.:
Clinicopathologic findings in dogs and cats with ethylene glycol intoxication.
J Am Vet Med Assoc184:
37–41,
1984.
24.
TieglerD.J.ArnottH.J.:
X-ray diffraction and fine structural studies of crystals in the malpighian tubules of silkworms.
Nature235:
166–167,
1971.
25.
TozukaK.KonjikT.SudoT.:
Study of passed stones by means of X-rays, infrared and thermal analysis.
J Urol130:
1119–1122,
1983.