High prevalence of vitamin D deficiency in pregnant women and its relationship with adverse pregnancy outcomes in Guizhou,China

Abstract

Objective

This study was performed to investigate the prevalence of vitamin D deficiency in pregnant women in Guizhou, China and its correlation with adverse infant and maternal outcomes during the perinatal period.

Methods

In total, 220 pregnant women who received perinatal care and delivered in the Affiliated Hospital of Guizhou Medical University from November 2014 to April 2015 were enrolled. Each woman’s serum vitamin D concentration was tested during early pregnancy, and its correlation with adverse infant and maternal outcomes in the perinatal period was analyzed.

Results

The mean serum vitamin D concentration was 29.5 ± 5.8 nmol/L. More than 90% of pregnant women had vitamin D insufficiency. Additionally, 38.4% of women with vitamin D deficiency and 22.2% with vitamin D inadequacy developed adverse perinatal outcomes. The vitamin D level was negatively correlated with adverse pregnancy outcomes.

Conclusion

Vitamin D deficiency is highly prevalent among pregnant women in Guizhou, China. The incidence of adverse perinatal outcomes was far higher in association with vitamin D deficiency than sufficiency. A negative correlation was found between the vitamin D level and the incidence of adverse perinatal outcomes in pregnant women. Therefore, targeted screening and proper supplementation are needed during early pregnancy.

Keywords

Vitamin D pregnancy nutrition China deficiency maternal outcomes

Introduction

Vitamin D is a fat-soluble vitamin and steroid derivative. By itself, it has no physiological function; however, when converted into its active forms, vitamin D becomes an effective substance with many physiological activities. The active forms of vitamin D include 25-hydroxyl vitamin D₃, 1,25-hydroxyl vitamin D₃, and 24,25-hydroxyl vitamin D₃. Among these, 1,25-hydroxyl vitamin D₃ is the main form, with an active rate 500 to 1000 times higher than that of 25-hydroxyl vitamin D₃.¹ However, because of its longer half-life, 25-hydroxyl vitamin D₃ is considered the best biomarker of the vitamin D status. As an essential nutrient for the human body, vitamin D has the following main physiological functions: regulation of calcium and phosphorus metabolism, promotion of bone growth, and regulation of cell growth and differentiation. Studies have revealed that vitamin D deficiency is a worldwide problem.^2–6 Furthermore, vitamin D deficiency and inadequacy are very common during pregnancy,^7,8 and their incidence can reach up to 96.0% to 99.4%.^9,10 Studies have also shown that vitamin D deficiency in pregnant women is associated with various adverse pregnancy outcomes in the mother (preeclampsia, gestational diabetes, cesarean section, and infectious disease), fetus (gestational duration), and offspring (type 1 diabetes, eczema, and inflammatory and atopic disorders).^11–14 Previous studies have suggested that vitamin D deficiency among pregnant women is common in China.^15,16 However, the vitamin D status in the general population varies significantly among different areas of China because of diverse diets, dietary supplements, and sunlight exposure.⁶ Therefore, the present study was designed to investigate the prevalence of vitamin D deficiency in pregnant women in Guizhou, China and explore the relationship between the vitamin D nutritional status and adverse perinatal outcomes in pregnant women.

Methods

Sampling

Pregnant women who received perinatal care and delivered in the Obstetric Department of the Affiliated Hospital of Guizhou Medical University from 1 November 2014 to 30 April 2015 were enrolled in this retrospective study. The patients’ baseline characteristics (age, gestational age, perinatal outcomes, and other parameters) were obtained from their medical records. The adverse perinatal outcomes assessed in this study were preeclampsia, gestational diabetes, and similar conditions. All pregnant women were divided into two groups according to their pregnancy outcomes: the healthy group (Group A), which comprised patients who had good pregnancy outcomes and no pregnancy complications, and the adverse perinatal outcome group (Group B), which comprised patients who were healthy before their pregnancy and developed adverse outcomes during the perinatal period. Patients with the following diseases were excluded: primary hypertension, primary diabetes, chronic liver disease, hyperthyroidism, and connective tissue diseases. All participants provided written informed consent to participate in the study. This study was approved by the medical ethics committee of GuiZhou Provincial Hospital.

Analysis of samples

All participants received health education and guidance regarding standard clinical nutrition during perinatal care. With the patients’ consent, 5 mL of fasting blood samples were collected from the antecubital vein in the early morning at 11 to 16 weeks of gestation and analyzed by a laboratory technician in the clinical laboratory of our hospital. The serum 25-hydroxyvitamin D [25-(OH)D] concentration was measured by enzyme-linked immunosorbent assay (Roche, Basel, Switzerland) according to the manufacturer’s guidelines. According to the standard set by the National Institutes of Health, a serum 25-(OH)D concentration of <30 nmol/L (<12 ng/mL) was defined as vitamin D deficiency, that from 30 to 50 nmol/L (12 to 20 ng/mL) was defined as vitamin D inadequacy, and that of >50 nmol/L (>20 ng/mL) was defined as vitamin D sufficiency.

Statistical methods

Measurement data are expressed as mean ± standard deviation. Data were analyzed using the statistical software SPSS 21.0 (IBM Corp., Armonk, NY, USA). The Wilcoxon signed-rank test and the Spearman rank correlations method were used to analyze the relationship between vitamin D and perinatal outcomes. The Wilcoxon signed-rank sum test is a nonparametric statistical hypothesis test and was used to compare the population mean ranks between Groups A and B. The Spearman rank correlations method was used to measure the strength and direction of the association between vitamin D levels in pregnant women in early pregnancy and perinatal infant and maternal outcomes. A P value of <0.05 was considered statistically significant.

Results

Baseline characteristics

In total, 220 pregnant women were enrolled in this study. Among these 220 women, 149 (67.7%) were assigned to Group A and 71 (32.3%) were assigned to Group B. The patients’ ages ranged from 18 to 43 years (mean, 29.3 ± 4.5 years). Four (1.8%) women were 18 to 21 years old, 188 (85.5%) were in 22 to 34 years old, and 28 (12.7%) were >35 years old. The serum 25-(OH)D concentration was measured from 11 to 13 gestational weeks. The differences in the mean age and gestational weeks between Groups A and B were not statistically significant (Table 1).

Table 1.

Comparison of baseline characteristics of Groups A and B.

	Group		P
	A	B	P
Age in years
18–21	3	1	0.75
22–34	125	63
≥35	21	7
Age in years
	28.35 ± 5.40	29.68 ± 4.30	0.0704
Gestational weeks
11	51	25	0.89
12	53	23
13	45	23

Data for Groups A and B are presented as number of patients or mean ± standard deviation.

Prevalence of vitamin D deficiency in pregnant women

The mean serum 25-(OH)D concentration among all 220 pregnant women was 29.5 ± 5.8 nmol/L. Vitamin D deficiency was found in 159 (72.3%) women, vitamin D inadequacy was found in 45 (20.5%) women, and vitamin D sufficiency was found in 16 (7.3%) women. Thus, overall, 92.8% of pregnant women in this study had vitamin D insufficiency.

Relationship between vitamin D level and perinatal outcomes

In Group A, vitamin D deficiency was found in 103 (69.1%) women, vitamin D inadequacy was found in 30 (20.1%) women, and vitamin D sufficiency was found in 16 (10.8%) women. In Group B, vitamin D deficiency was found in 61 (85.9%) women and vitamin D inadequacy was found in 10 (14.1%) women; no women in Group B had vitamin D sufficiency. The Wilcoxon signed-rank test showed that the differences between these two groups were statistically significant (P < 0.05). Overall, 38.4% of pregnant women with vitamin deficiency and 22.2% of pregnant women with vitamin inadequacy developed adverse perinatal outcomes.

Spearman’s correlation analysis was performed to further explore the relationship between vitamin D levels in pregnant women in early pregnancy and perinatal infant and maternal outcomes. The results revealed that the vitamin D level was negatively correlated with adverse pregnancy outcomes (Spearman correlation = −0.433, P < 0.05).

Discussion

Vitamin D is a fat-soluble vitamin. Its deficiency increases the risk of metabolic syndromes, including abnormal glucose metabolism, obesity, hypertension, and cardiovascular and cerebrovascular disease.² For example, vitamin D deficiency increases the incidence of preeclampsia and the rate of cesarean section, and it is closely associated with gestational age, neonatal hypoglycemia and hyperinsulinemia,^17,18 and decreased birth height, weight, and head circumference of the offspring.¹⁹

Studies have revealed that the average 25-(OH)D concentration in pregnant women in early pregnancy in the Middle East is <25 nmol/L.^20,21 In China, a study conducted in the Beijing area revealed that the mean 25-(OH)D concentration in healthy women was 27.28 ± 6.64 nmol/L; women with 25-(OH)D deficiency and inadequacy accounted for 99.4%, and women with adequate levels of 25-(OH)D accounted for only 0.6%.⁶ Wang et al.²² reported that the mean serum 25-(OH)D level in pregnant women was 38.54 ± 17.18 nmol/L in the urban district of Xi'an city, Shanxi Province, and nearly 90% of pregnant women had 25-(OH)D deficiency. In addition, Xie et al.²³ reported that in Nanjing city, Jiangsu Province, the mean 25-(OH)D concentration in pregnant women was 26.4 ± 10.7 nmol/L in summer and 22.7 ± 4.8 nmol/L in winter. These studies showed that >96% of pregnant women had low levels of vitamin D. Because the vitamin D status in pregnant women varies prominently among different areas because of different diets, dietary supplements, and sunlight exposure,⁶ the present study was performed to determine the vitamin D status in pregnant women in Guizhou, China. We found that the mean level of vitamin D in pregnant women was 29.5 ± 5.8 nmol/L, and >90% of pregnant women had vitamin D insufficiency. Furthermore, the incidence of adverse perinatal outcomes was significantly higher in pregnant women with vitamin D deficiency than in women with vitamin D sufficiency. In addition, the vitamin D level in pregnant women was negatively correlated with the incidence of adverse perinatal outcomes. Therefore, for adequate prenatal care, guidance regarding nutrition and appropriate interventions are necessary during pregnancy. However, no unified standard guidelines on supplementation of vitamin D during pregnancy are currently available. The American College of Obstetricians and Gynecologists recommends that for women with risk factors (vegetarians, populations with inadequate sun exposure due to living in the north or wearing protective clothing, and ethnicity, especially women with dark skin), daily supplementation with 1000 to 2000 IU of vitamin D is safe.²⁴ However, no adequate studies have been performed to evaluate the safety of large doses of additional supplements. For women without an increased risk of vitamin D deficiency, enough vitamin D can be obtained by taking prenatal vitamins. In 2010, the US Institute of Medicine established that daily supplementation with 600 IU of vitamin D is sufficient for pregnant and lactating women. Most prenatal vitamins contain at least 400 IU of vitamin D per tablet. The 2013 version of the “dietary reference intake for Chinese residents” recommends that the reference nutrient intake value of vitamin D in pregnant women in early pregnancy is 10 µg/day (1 IU = 0.025 µg; that is, 400 IU/day). Whether this reference value can meet the requirement of pregnant women needs to be further verified in the clinical setting.

Although this study provides a glimpse of the vitamin D status of pregnant women in Guizhou, China, the sample size was rather small. Moreover, this study was conducted in one hospital, which may have led to bias. Therefore, to more accurately guide the daily intake of vitamin D in pregnant women and further decrease adverse pregnancy outcomes from a nutritional perspective, multicenter prospective cohort studies are needed to guide perinatal nutrition and health care.

Footnotes

Declaration of conflicting interest

The authors declare that there is no conflict of interest.

Funding

The authors disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: Science Fund: Qiankehe LH zi [2014] 7125 and Qiankehe LH zi [2016] 7137.

References

Reichel

Koeffler

Norman

AW.

The role of the vitamin D endocrine system in health and disease.

N Engl J Med 1989; 320: 980–991.

Neyestani

Hajifaraji

Omidvar

et al . High prevalence of vitamin D deficiency in school-age children in Tehran, 2008: a red alert. Public Health Nutr 2012; 15: 324–330.

Ning

Song

Miao

et al . High prevalence of vitamin D deficiency in urban health checkup population. Clin Nutr 2016; 35: 859–863.

Bener

Al-Ali

Hoffmann

GF.

High prevalence of vitamin D deficiency in young children in a highly sunny humid country: a global health problem. Minerva Pediatr 2009; 61: 15–22.

Boonman-de Winter

Albersen

Mohrmann

et al . [High prevalence of vitamin D deficiency in the south-west Netherlands]. Ned Tijdschr Geneeskd 2015; 159: A8167.

Holick

MF.

Vitamin D deficiency.

N Engl J Med 2007; 357: 266–281.

Sachan

Gupta

Das

et al.

High prevalence of vitamin D deficiency among pregnant women and their newborns in northern India.

Am J Clin Nutr 2005; 81: 1060–1064.

Dawodu

Wagner

CL.

Mother-child vitamin D deficiency: an international perspective.

Arch Dis Child 2007; 92: 737–740.

Holmes

Barnes

Alexander

et al . Vitamin D deficiency and insufficiency in pregnant women: a longitudinal study. Br J Nutr 2009; 102: 876–881.

10.

Yang

Zhang

Fan

et al.

The relationship between serum 25-hydroxyvitamin D levels and insulin resistance of healthy childbearing age women in Beijng city. Chinese Journal of Clinical Obstetrics and Gynecology 2012; 13: 263–266.

11.

Dror

DK.

Vitamin D status during pregnancy: maternal, fetal, and postnatal outcomes.

Curr Opin Obstet Gynecol 2011; 23: 422–426.

12.

Dror

Allen

LH.

Vitamin D inadequacy in pregnancy: biology, outcomes, and interventions.

Nutr Rev 2010; 68: 465–477.

13.

Bodnar

Simhan

Powers

et al.

High prevalence of vitamin D insufficiency in black and white pregnant women residing in the northern United States and their neonates.

J Nutr 2007; 137: 447–452.

14.

Bodnar

Krohn

Simhan

HN.

Maternal vitamin D deficiency is associated with bacterial vaginosis in the first trimester of pregnancy.

J Nutr 2009; 139: 1157–1161.

15.

Chen

Hao

et al . Influent factors of gestational vitamin D deficiency and its relation to an increased risk of preterm delivery in Chinese population. Sci Rep 2018; 8: 3608.

16.

Wang

Yang

Mao

et al.

High prevalence of vitamin D and calcium deficiency among pregnant women and their newborns in Chengdu, China.

World J Pediatr 2010; 6: 265–267.

17.

HAPO Study Cooperative Research Group, Metzger

Lowe

et al . Hyperglycemia and adverse pregnancy outcomes. N Engl J Med 2008; 358:1991–2002.

18.

Sun

Yang

Sun

WJ.

Risk factors for pre-eclampsia in pregnant Chinese women with abnormal glucose metabolism.

Int J Gynaecol Obstet 2008; 101: 74–76.

19.

Song

Liu

et al . Vitamin D status in Chinese pregnant women and their newborns in Beijing and their relationships to birth size. Public Health Nutr 2013; 16: 687–692.

20.

Lips

Worldwide status of vitamin D nutrition.

J Steroid Biochem Mol Biol 2010; 121: 297–300.

21.

Bassil

Rahme

Hoteit

et al . Hypovitaminosis D in the Middle East and North Africa: Prevalence, risk factors and impact on outcomes. Dermatoendocrinol 2013; 5: 274–298.

22.

Wang

Liu

YF.

Effect of vitamin D deficiency among urban pregnant women on their neonates in Xi'an. Chinese Journal of Woman and Child Health Research 2012; 23: 20–22.

23.

Xie

Jiang

et al . Differences in the levels of serum 25 - hydroxyl vitamin D of pregnant women in winter and summer in nanjing area. Chinese Journal of Perinatal Medicine 2013; 16: 100–101.

24.

ACOG Committee on Obstetric Practice. ACOG Committee Opinion No. 495: Vitamin D: Screening and supplementation during pregnancy. Obstet Gynecol 2011; 118: 197–198.