Environmental mercury and mercury compound contamination has increased dramatically since the industrial revolution. This paper describes the toxic effects of mercury on a culture of hybridoma TA7 cells, which produce antibodies against the A-subunit of viskumin. Cells were cultivated on 96-well flat-bottomed plates with RPMI-1640 medium supplemented with 10% fetal calf serum at 37°C in 5% CO2/95% air. The cells were exposed to 0.1nM/l–10μM/l Hg2(NO3)2.2H2O (mercury nitrate) during the exponential growth phase. Toxicity was assessed by using the colorimetric MTT (tetrazolium) assay after exposure for 48 hours. Cell growth and cell survival were evaluated by using percentage indices of cellular content in exposed cells when compared to non-exposed control cells. The concentrations of the no-effect level, the lowest observed effect level and the the highest toxic effect level were registered. The toxic effects of the mercury compound on the hybridoma cells occurred between 0.1μM/l and 10μM/l.
OliveroJ., & SolanoB. (1998). Mercury in environmental samples from water body contaminated by gold mining in Colombia, South America. Science of the Total Environment217, 83–89.
2.
MionD., & PierinA.M. (1998). How accurate are sphygnomanometers?Journal of Human Hypertension12, 245–248.
3.
KuligK., RumackC.M., RumackB.H., & DuffyJ.P. (1983). Disk battery ingestion elevated urine mercury levels and enema removal of battery fragments. Journal of the American Medical Association249, 1202–1204.
4.
JoselowM.M., LouriaD.B., & BrowderA.A. (1972). Mercuralism: environmental and occupational aspects. Annals of Internal Medicine76, 119–130.
5.
JoensuuO.I. (1971). Fossil fuels as a source of mercury pollution. Science, New York172, 1027–1028.
6.
DickmanM.D., LeugC.K., & LeongM.K. (1998). Hong Kong's male subfertility links of mercury in human hair and fish. Science of the Total Environment214, 165–174.
7.
HughesJ., & IngramB.J. (1995). The concern over mercury and wastewater. Journal of the Michigan Dental Association77, 28–29.
8.
KostiniakP.J. (1998). Mercury as a potential hazard for the dental practitioner. Review. New York State Dental Journal64, 40–43.
9.
Medical Statistical Bureau of Latvia (1999). Annual Book of Medical Statistical Bureau of Latvia42, 46–56 (Latvian).
10.
SalonenJ.T. (1998). Excessive intake in iron and mercury in cardiovascular disease. Review. Bibliotheca Nutritio et Dieta54, 112–126.
11.
DeleuD., HanssensY., al-SalmyH.S., & HastieI. (1998). Peripheral polineuropathy due to chronic use of topical ammoniated mercury.36, 233–237.
12.
EkwallB. (1998). MEIC evaluation of acute systemic toxicity. Part VI. The prediction of human toxicity by rodent LD50 values and results from 61 in vitro methods. ATLA26, Suppl. 2, 617–658.
13.
ClemedsonC. (1996). MEIC valuation of acute systemic toxicity. Part I. Methodology of 68 in vitro toxicity assays used to test the first 30 reference chemicalsATLA, Suppl. 1, 251–272.
14.
HaitovR.M., SidorovichI.G., & IgnatyevaG.A. (1987). The contemporary aspects of hybridoma's technology. Medicine and Public Health Series: The reviews about important problems of medicine. Moscow, BHUUMU, Part 4, 65 (Russian).
15.
MaluchenkoN.B., MoisenovichM.M., JegorovaS.G., KolesnikovaJ.F., AgapovJ.I., SolopovaO.C., ZaicevI.Z., & TonevickijA.G. (1999). A-sub-units of viskumin: the opening in the trend of development of the intracellular transport of toxin. Journal of Molecular Biology5, 20–23 (Russian).
16.
MosmannT. (1983). Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. Journal of Immunological Methods65, 55–63.
17.
RemezI., VasilyevaS., & AndersonsP. (1995). Some modern methods in ecotoxicology.Eighth Scandinavian Workshop on In Vitro Toxicology 1995. Tampere, Finland. Abstract 131.
18.
TimbrellJ.A. (1998). Biomarkers in toxicology. Toxicology129, 1–12.
19.
PurchaseI.F.H., BothamP.A., BrunerL.H., FlintO., FraziertJ., & StokesW. (1998). Scientific and regulatory challenges for the reduction, refinement and replacement of animals in toxicity testing. Workshop overview. Toxicological Science43, 86–101.
20.
SetumC., & MathurA. (1993). Effect of host immune stimulation on B53 hybridoma growth and IG regulation. Journal of Immunology150, 8, Part II, Abstract 143.
21.
GomiH., TagayaY., MikayamaT., & IshuzakuK. (1993). Antigen-binding glycosylation inhibition factor (GIF) from a human T cell hybridoma specific for bee venom phospholipase A2 constructed from T cell of a honey bee venom-sensitive allergic individual, resulted in formation GIF having affinity for bee venom. Journal of Immunology150, 8, Part II, Abstract 47.
22.
MoriA., ThomasY., TagayaY., & JangE. (1993). Epitope specificity of bee venom phospolipase A2 specific T-hybridomas which produce antigen-binding glycosylation inhibiting factor. Journal of Immunology150, 8, Part II, Abstract 289.
23.
SuzukiR., FoxF.E., SuzukiS., & LiA. (1993). Purification and characterization of human T-T cell hybridoma-derived suppressor factor. Journal of Immunology150, 8, Part II, Abstract 131.
24.
DesaulniersK., LeingartnerK., ZachazevskyT., & FosterW.G. (1998). Optimization of an MCF7-E3 cell proliferation assay and effect of environmental pollutants and industrial chemicals. Toxicology in Vitro12, 409–421.
25.
IrvinA.J.E., EarlL.K., JonesP.A., & BakerV.A. (1998). Interpretation of the in vitro proliferation response of MCF-7 cells to potential oestrogens and non-oestrogenic substances. Toxicology in Vitro12, 373–382.
26.
OdumJ., TitensorS., & ShulerM.L. (1998). Limitation of the MCE-7 cell proliferation assay for derived xenobiotic oestrogen. Toxicology in Vitro12, 273–278.
27.
BushM.L., FrederickC.B., KimbellJ.S., & UltmanJ.S. (1998). CFD-PBPK hybrid model for simulating gas and vapour uptake in the rat's nose. Toxicology and Applied Pharmacology150, 133–145.
28.
KeisariY. (1992). A colorimetric microtiter assay for the quantitation of cytokine activity on adherent cells in tissue culture. Journal of Immunological Methods146, 155–161.
29.
ChibaK., KawakamiK., & TonyamiK. (1998). Simultaneous evaluaton of cell viability by neutral red, MTT and crystal violet staining assay of the same cell. Toxicology in Vitro12, 251–258.
