5-Bromodeoxyuridine (5-BrdU), a pyrimidine analogue of thymidine, is incorporated into the DNA which is actively synthesised during the S-phase of the cell cycle. In the present study, the labelling indices of cultured human keratinocytes, marked by monoclonal antibodies to 5-BrdU and by tritiated thymidine (3H-TdR), were compared.
The proportion of cells found to be labelled by the two methods was similar. The immunological method has advantages over 3H-TdR autoradiography, because it does not require radioactive material, and is both faster and less expensive.
PrunierasM., and DelescluseC. (1984). Epidermal cell culture systems in skin pharmacology.British Journal of Dermatology111 Suppl. 27, 43–57.
2.
GratznerH.G. (1982). Monoclonal antibody to 5-bromo- and 5-iododeoxyuridine: a new reagent for detection of DNA replication.Science218, 474–475.
3.
GonchoroffN.J., GreippP.R., KyleR.A., and KatzmannJA. (1985). A monoclonal antibody reactive with 5-bromo-2-deoxyuridine that does not require DNA denaturation.Cytometry6, 506–512.
4.
RheinwaldJ.G., and GreenH. (1975). Serial cultivation of strains of human epidermal keratinocytes: the formation of keratinizing colonies from single cells.Cell6, 331–344.
5.
GreenH., KehindeO., and ThomasJ. (1979). Growth of cultured human epidermal cells into multiple epithelia suitable for grafting.Proceedings of the National Academy of Science of the United States of America76, 5665–5668.
6.
SacchiS., DonelliA., CocconcelliP., EmiliaG., MesserottiA., PiccininiL., SelleriL., TorelliG., RinaldiG., and TorelliU. (1985). Monoclonal antibody to 5-bromodeoxyuridine: a sensitive and rapid method for estimating the amount of S-phase cells. In Biotechnology in Diagnostics (ed. KoprowskiH., FerroneS., AlbertiniA.), pp. 65–70. New York: Elsevier Science Publishers.
7.
OkuT., TakigawaM., and YamadaM. (1987). Cell proliferation kinetics of cultured human keratinocytes and fibroblasts measured using a monoclonal antibody.British Journal of Dermatology116, 673–679.
8.
DolbeareF., GratznerH., PallaviciniM.G., and GrayJ.W. (1983). Flow-cytometric measurement of total DNA content and incorporated bromodeoxyuridine.Proceedings of the National Academy of Science of the United States of America80, 5573–5577.
