5-Bromodeoxyuridine (5-BrdU), a pyrimidine analogue of thymidine, is incorporated into the DNA which is actively synthesised during the S-phase of the cell cycle. In the present study, the labelling indices of cultured human keratinocytes, marked by monoclonal antibodies to 5-BrdU and by tritiated thymidine (3H-TdR), were compared.
The proportion of cells found to be labelled by the two methods was similar. The immunological method has advantages over 3H-TdR autoradiography, because it does not require radioactive material, and is both faster and less expensive.
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