In this half-day session of the Society of Toxicologic Pathology Annual meeting, we focused on hot topics, future directions, and challenges faced in toxicologic neuropathology. The panel of speakers addressed issues with direct delivery of agents to the brain, the dilemma of cervical dorsal root ganglia collection, and new approaches using digital pathology techniques for assessment of the nervous system.
AbelJTOuillettePWilliamsCL, et al. Display characteristics and their impact on digital pathology: a current review of pathologists’ future “microscope.”J Pathol Inform. 2020;11(1):23.
2.
BehanovaAAbdollahzadehABelevichIJokitaloESierraATohkaJ.GACSON software for automated segmentation and morphology analyses of myelinated axons in 3D electron microscopy. Comput Methods Programs Biomed. 2022;220:106802.
3.
BerbísMÁ. Digital and computational pathology: what a time to be alive!Mayo Clin Proc Digit Health. 2023;2(1):18-20.
4.
BolonBBuzaEGalbreathEWicksJCargninFHordeauxJ.Neuropathological findings in nonclinical species following administration of adeno-associated virus (AAV)-based gene therapy vectors. Toxicol Pathol. 2024;52(8):489-505.
5.
BolonBGarmanRJensenKKrinkeGStuartB; Ad Hoc Working Group of the STP Scientific and Regulatory Policy Committee. A “best practices” approach to neuropathologic assessment in developmental neurotoxicity testing—for today [Erratum in: Toxicol Pathol.2006;34(5):697. PMID: 16698729]. Toxicol Pathol. 2006;34(3):296-313.
6.
Carrillo-BarberàPRondelliAMMorante-RedolatJMVernayBWilliamsABankheadP.AimSeg: a machine-learning-aided tool for axon, inner tongue and myelin segmentation. PLoS Comput Biol. 2023;19(11):e1010845.
7.
ChenZShinDChenS, et al. Histological quantitation of brain injury using whole slide imaging: a pilot validation study in mice. PLoS ONE. 2014;9(3):e92133.
8.
CourtneyJMMorrisGPClearyEMHowellsDWSutherlandBA. An automated approach to improve the quantification of pericytes and microglia in whole mouse brain sections. eNeuro. 2021;8(6):ENEURO.0177-21.2021.
9.
EschPMDonzéGEschbachB, et al. Good Laboratory Practice (GLP)– guidelines for the validation of computerised systems. Qual Assur J. 2007;11(3-4):208-220.
GutierrezSHuynhTIwanagaJDumontASBuiCJTubbsRS.A review of the history, anatomy, and development of the C1 spinal nerve. World Neurosurg. 2020;135:352-356.
12.
HaberbergerRVBarryCDominguezNMatusicaD.Human dorsal root ganglia. Front Cell Neurosci. 2019;13:271.
13.
HannaMGReuterVESamboyJ, et al. Implementation of digital pathology offers clinical and operational increase in efficiency and cost savings. Arch Pathol Lab Med. 2019;143(12):1545-1555.
14.
IkedaMOkaY.The relationship between nerve conduction velocity and fiber morphology during peripheral nerve regeneration. Brain Behav. 2012;2(4):382-390.
15.
JacobsenMLewisABailyJFraserARudmannDRyanS.Utilizing whole slide images for the primary evaluation and peer review of a GLP-compliant rodent toxicology study. Toxicol Pathol. 2021;49(6):1164-1173.
16.
MeseckEKGuibingaGWangSMcElroyCHudryEMansfieldK.Intrathecal sc-AAV9-CB-GFP: systemic distribution predominates following single-dose administration in cynomolgus macaques. Toxicol Pathol. 2022;50(4):415-431.
17.
KaiserTAllenHMKwonO, et al. MyelTracer: a semi-automated software for myelin g-ratio quantification. eNeuro. 2021;8:ENEURO.0558-20.2021. Accessed August 19, 2025. https://www.eneuro.org/content/8/4/ENEURO.0558-20.2021.
18.
NahalABatacCMSlawRJBauerTW.Setting up an ePathology service at Cleveland clinic Abu Dhabi: joint collaboration with Cleveland clinic, United States. Arch Pathol Lab Med. 2018;142(10):1216-1222.
19.
Pohlmeyer-EschGHalseyCBoisclairJ, et al. Digital pathology and artificial intelligence applied to nonclinical toxicology pathology-the current state, challenges, and future directions. Toxicol Pathol. 2025;53(6):516-535.
RetameroJAAneiros-FernandezJDel MoralRG.Complete digital pathology for routine histopathology diagnosis in a multicenter hospital network. Arch Pathol Lab Med. 2020;144(2):221-228.
22.
SchaferKARaoDB.Toxicologic pathology forum: opinion on digital primary read and peer review-are we there yet?Toxicol Pathol. 2025;53(2):210-214.
23.
StetzikLMercadoGSmithL, et al. A novel automated morphological analysis of Iba1+ microglia using a deep learning assisted model. Front Cell Neurosci. 2022;16:944875.
24.
StoffelMHOevermannAVandeveldeM.Fundamental Neuropathology for Pathologists and Toxicologists: Principles and Techniques. Hoboken, NJ: Wiley; 2011.
25.
ThomasCECombsCM.Spinal cord segments. B. Gross structure in the adult monkey. Am J Anat. 1965;116(1):205-216.
26.
ThomsonBRMartinLFSchmidlePLSchlierbachHSchänzerARichterH.Automated pipeline for nerve fiber selection and g-ratio calculation in optical microscopy: exploring staining protocol variations. Front Neuroanat. 2023;17:1260186.
27.
VandenbergheMEHérardASSouedetN, et al. High-throughput 3D whole-brain quantitative histopathology in rodents. Sci Rep. 2016;6(1):20958.
28.
WatsonCKayaliogluG. The organization of the spinal cord. In: WatsonCPaxinosGKayaliogluG, eds. The Spinal Cord, A Christopher and Dana Reeve Foundation Text and Atlas. Amsterdam: Elsevier; 2009:1-7.