The chemical identification of botanical products can be accomplished by obtaining a “profile” of the mixture components. Changes in this profile are powerful indicators of variations due to such factors as process differences, raw material source, or storage stability. Such a profile may be obtained using an analytical protocol based on liquid chromatography/mass spectrometry (LC/MS) techniques. This paper describes an analytical strategy using LC/MS which can be used to rapidly characterize the components in complex materials and to support their research, development, and manufacturing. This strategy utilizes standard method approaches, a rapid “template” approach for structure identification, and the application of databases of compounds to facilitate identification and consistency throughout the product lifetime. This analytical strategy is illustrated with the LC/MS characterization of mixtures of taxanes from Taxus Brevifolia extracts and development of a taxane database. This LC/MS-based strategy provides effective information for the accelerating medicinal field.
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