Paper spray ionization (PSI) has emerged as a key ambient ionization technique in microanalysis over the past decade, valued for its ease of use and minimal sample preparation. This study reviews PSI's applications in forensic science, covering areas such as biomolecules, drugs of abuse, inks, explosives, toxicology, and trace evidence. To achieve these objectives, electronic searches were performed across several databases—Scopus, Web of Science, Science Direct, and PubMed—covering publications from 2010 through December 2023, with a focus on English-language articles. This search strategy yielded 40 relevant studies, which were reviewed to assess PSI-MS's current capabilities and advancements. The review underscores Paper Spray Ionization Mass Spectrometry's (PSI-MS) versatility, particularly its compatibility with various sampling methods like dried blood spots, which enhances its utility for rapid point-of-care (POC) analysis. This capability facilitates the efficient analysis of biochemical and forensic samples while bypassing lengthy separation procedures, marking a significant advancement in forensic analysis techniques.
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