Few studies consider the effects of impaction, resulting in inaccurate estimation of age using third molar. We aimed to assess the significance of impaction in estimating age using Modified Demirjian’s method. 200 orthopantomograms of patients between 16–25 years were collected comprising 171 tests and 212 controls. Chronologic age (CA) and estimated age (EA) were calculated. Independent sample t-test, chi-square test, paired t-test and Kolmogrov-Smirnov test of normality were used. The mean difference between the CA and EA in both the tests and control samples were significant (mean difference test group = 2.6 ± 2.07, control group = 2.0 ± 1.8; p = 0.01). It provided a difference of 0.6 which is equal to 7.2 months. The results of our study indicate that impacted mandibular third molars are susceptible to undergo slower mineralization, leading to erroneous lower age estimation. These results in an Indian population are noteworthy. However further studies are needed.
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