Altered Effect of Arachidonic Acid on Inner Ear Blood Flow in Rats with Streptozotocin-Induced Diabetes

Abstract

The present study was undertaken to clarify the altered effect of arachidonic acid on inner ear blood flow in rats with streptozotocin-induced diabetes by use of the laser-Doppler flowmeter. Drugs were administered intraarterially via the subclavian artery in a dose range that did not affect the systemic blood pressure. Both arachidonic acid and papaverine hydrochloride increased inner ear blood flow dose-dependently. Diabetic rats at 12 weeks, but not at 8 weeks, after the induction of diabetes showed a significant decrease in arachidonic acid response. However, there were no differences in papaverine response between diabetic and control rats. Pretreatment with ONO-3708, a selective thromboxane A2 antagonist, reversed the attenuated response to arachidonic acid found in diabetic rats. An increased response to thromboxane A2, which decreased inner ear blood flow, was also found in 12-week diabetic rats. In electrocochleograms, the latency in 12-week diabetic rats was significantly delayed compared with that in control rats, and this prolonged latency improved with insulin treatment. These results suggest that the responsiveness of inner ear blood flow to prostaglandins may be altered in individuals with diabetes mellitus.

Keywords

diabetic hearing disorders inner ear microcirculation prostaglandins

Get full access to this article

View all access options for this article.

References

Jorgensen

. Studies on inner-ear function and cranial nerves in diabetics. Acta Otolaryngol (Stockh) 1961; 53: 350–64.

Friedman

. Hearing and diabetic neuropathy. Arch Intern Med 1975; 135: 573–6.

Axelsson

. Hearing in diabetics. Acta Otolaryngol [Suppl] (Stockh) 1987(suppl 356).

Taylor

. Some audiological aspects of diabetes mellitus. J Laryngol Otol 1978; 92: 99–113.

Jordao

. Consideration sur un cas du diabete. Um Med Paris 1857; II: 466–540.

Zelenka

. Disorder in blood supply of the inner ear as early symptom of diabetic angiopathy. J Laryngol Otol 1961; 79: 314–9.

Roren

. Microangiopathy in diabetics with hearing disorder. Eye Ear Nose Throat Mon 1971; 50: 479–82.

Makishima

. Pathological changes of the inner ear and central auditory pathway in diabetic. Ann Otol Rhinol Laryngol 1971; 80: 218–28.

Costa

. Inner ear pathology in experimental diabetes. Laryngoscope 1967; 77: 68–75.

10.

Wackym

. Diabetes mellitus and hearing loss: Clinical and histopathologic relationships. Am J Otolaryngol 1986; 3: 176–82.

11.

Jorgensen

. The inner ear in diabetes mellitus. Arch Otolaryngol 1961; 74: 373–81.

12.

Moncada

Vane

. Arachidonic acid metabolites and the interactions between platelets and blood vessel walls. N Engl J Med 1979; 300: 1142–7.

13.

Escoubet

. Prostaglandin synthesis by cochlea of the guinea pig. Influence of aspirin, gentamicin, and acoustic stimulation. Prostaglandins 1985; 29: 589–99.

14.

Ba Huy

P Tran

. Prostaglandins and leukotrienes. Acta Otolaryngol (Stockh) 1987; 103: 558–66.

15.

Umemura

Takiguchi

Nakashima

Nozue

. Effect of arachidonic acid on the inner ear blood flow measured with a laser Doppler flowmeter. Ann Otol Rhinol Laryngol 1990; 99: 491–5.

16.

Colwell

. Platelet function in diabetes mellitus. Br J Haematol 1980; 44: 521–6.

17.

Viinikka

. Platelet function and thrombus in diabetes. Acta Endocrinol (Copenh) 1985; 110(suppl 272): 31–4.

18.

Jonathan

. Alteration in the balance of prostaglandin and thromboxane synthesis in diabetic rats. J Lab Clin Med 1980; 95: 950–8.

19.

Miwa

Okuda

Maeda

Okuda

. Mutarotase effect on colorimetric determination of blood glucose with -D-glucose oxidase. Clin Chim Acta 1972; 37: 538–40.

20.

Fujioka

Nagao

Kuriyama

. Actions of the novel thromboxane A2 antagonists, ONO-1270 and ONO-3708, on smooth muscle cells of the guinea-pig basilar artery. Naunyn Schmiedeberg's Arch Pharmacol 1986; 334: 468–74.

21.

Roth

Reibel

Lefer

. Vascular responsiveness and eicosanoid production in diabetic rats. Diabetologia 1983; 24: 372–6.