Note: Retention of Ascorbic Acid,Thiamin and Pyridoxal After High Hydrostatic Pressure or Thermal Treatments

Abstract

The retention of ascorbic acid, thiamin and pyridoxal was examined after high hydrostatic pressure (HHP) or thermal treatment in sodium-1-pentane sulfonate (SPS) buffer solutions at pH 2.2, 4.5 and 6.0. The 1 mg/1 mL solutions of ascorbic acid, thiamin or pyridoxal were prepared separately in SPS buffer solutions at selected pH and treated at a pressure of 689 MPa at 21°C for 30 min or thermally treated at 121°C for 30 min. Mean retention for each of the three vitamins in the SPS buffer solutions at the three pH after HHP and thermal treatments were compared. The retention of ascorbic acid, thiamin or pyridoxal in SPS buffer solutions after HHP treatment were 90.6 ± 9.7, 95.9 ± 4.3 and 93.2 ± 8.7, respectively. The retention of ascorbic acid in SPS buffer solutions at pH 4.5 or 6.0 was significantly greater after HHP treatment than after thermal treatment (p ≤ 0.05). The retention of thiamin in SPS buffer solutions was uniformly greater at all three pH after HHP treatment than after thermal treatment. The retention of pyridoxal in SPS buffer solutions at pH 2.2 or 4.5 was not statistically different (p > 0.05) after HHP or thermal treatments. Retention of pyridoxal in SPS buffer at pH 6.0 was significantly higher after HHP treatment than after thermal treatment (p ≤ 0.05).